Homogenous, real-time duplex loop-mediated isothermal amplification using a single fluorophore-labeled primer and an intercalator dye: Its application to the simultaneous detection of Shiga toxin genes 1 and 2 in Shiga toxigenic Escherichia coli isolates

Molecular and Cellular Probes

Volumn 24, Issue 4, 2010, Pages 190-195

  Kouguchi, Yoshihiro ^a   Fujiwara, Takako ^a   Teramoto, Miki ^a   Kuramoto, Mika ^a  

^a Fukuyama City Public Health Center   (Japan)

Author keywords

Ethidium bromide; LAMP; Multiplex detection; Simultaneous detection

Indexed keywords

6 CARBOXYFLUORESCEIN; ETHIDIUM BROMIDE; VEROTOXIN 1; VEROTOXIN 2;

ARTICLE; BACTERIAL STRAIN; BACTERIUM ISOLATE; FLUORESCENCE RESONANCE ENERGY TRANSFER; LOOP MEDIATED ISOTHERMAL AMPLIFICATION; NONHUMAN; PRIORITY JOURNAL; REAL TIME DUPLEX LOOP MEDIATED ISOTHERMAL AMPLIFICATION; SHIGA TOXIN PRODUCING ESCHERICHIA COLI;

DNA PRIMERS; ETHIDIUM; FLUORESCEINS; FLUORESCENT DYES; INTERCALATING AGENTS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; NUCLEIC ACID CONFORMATION; SENSITIVITY AND SPECIFICITY; SHIGA TOXIN 1; SHIGA TOXIN 2; SHIGA-TOXIGENIC ESCHERICHIA COLI; STAINING AND LABELING; TEMPERATURE; TIME FACTORS;

ESCHERICHIA COLI;

EID: 77954386893     PISSN: 08908508     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mcp.2010.03.001     Document Type: Article

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