A rapid, simple, and effective method of constructing a randomly mutagenized plasmid library free from ligation

Bioscience, Biotechnology and Biochemistry

Volumn 72, Issue 4, 2008, Pages 1134-1137

  Koyanagi, Takashi ^a   Yoshida, Erina ^a   Minami, Hiromichi ^a   Katayama, Takane ^a   Kumagai, Hidehiko ^a  

^a ISHIKAWA PREFECTURAL UNIVERSITY   (Japan)

Author keywords

type DNA polymerase; Manganese; Random mutagenesis

Indexed keywords

DNA; ENZYME ACTIVITY; ERROR ANALYSIS; GENES; MANGANESE;

COMPLEMENTATION ANALYSIS; RANDOM MUTAGENESIS; REACTION MIXTURE;

MUTAGENESIS;

MANGANESE;

ARTICLE; CHEMISTRY; GENE LIBRARY; GENETICS; METHODOLOGY; MUTATION; NUCLEOTIDE SEQUENCE; PLASMID; SITE DIRECTED MUTAGENESIS; STATISTICS; TIME;

BASE SEQUENCE; GENE LIBRARY; MANGANESE; MUTAGENESIS, SITE-DIRECTED; MUTATION; PLASMIDS; STOCHASTIC PROCESSES; TIME FACTORS;

EID: 43549125426     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.70814     Document Type: Article

References (15)

1. Cirino, P. C., Mayer, K. M., and Umeno, D., Generating mutant libraries using error-prone PCR. Methods Mol. Biol., 231, 3-9 (2003).
2. Sohn, H., Kim, Y. S., Jin, U. H., Suh, S. J., Lee, S. C., Lee, D. S., Ko, J. H., and Kim, C. H., Alteration of the substrate specificity of Thermus caldophilus ADP-glucose pyrophosphorylase by random mutagenesis through error-prone polymerase chain reaction. Glycoconj. J., 23, 619-625 (2006).
3. Khan, M. I., Ito, K., Kim, H., Ashida, H., Ishikawa, T., Shibata, H., and Sawa, Y., Molecular properties and enhancement of thermostability by random mutagenesis of glutamate dehydrogenase from Bacillus subtilis. Biosci. Biotechnol. Biochem., 69, 1861-1870 (2005).
4. Katayama, T., Suzuki, H., Koyanagi, T., and Kumagai, H., Cloning and random mutagenesis of the Erwinia herbicola tyrR gene for high-level expression of tyrosine phenol-lyase. Appl. Environ. Microbiol., 66, 4764-4771 (2000).
5. Spee, J. H., de Vos, W. M., and Kuipers, O. P., Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP. Nucleic Acids Res., 21, 777-778 (1993).
6. Beckman, R. A., Mildvan, A. S., and Loeb, L. A., On the fidelity of DNA replication: manganese mutagenesis in vitro. Biochemistry, 24, 5810-5817 (1985).
7. van Loo, B., Spelberg, J. H., Kingma, J., Sonke, T., Wubbolts, M. G., and Janssen, D. B., Directed evolution of epoxide hydrolase from A. radiobacter toward higher enantioselectivity by error-prone PCR and DNA shuffling. Chem. Biol., 11, 981-990 (2004).
8. Miyazaki, K., and Takenouchi, M., Creating random mutagenesis libraries using megaprimer PCR of whole plasmid. Biotechniques, 33, 1033-1038 (2002).
9. Fujii, R., Kitaoka, M., and Hayashi, K., One-step random mutagenesis by error-prone rolling circle amplification. Nucleic Acids Res., 32, e145 (2004).
10. Fujii, R., Kitaoka, M., and Hayashi, K., Error-prone rolling circle amplification: the simplest random mutagenesis protocol. Nat. Protoc., 1, 2493-2497 (2006).
11. Masamune, Y., and Richardson, C. C., Strand displacement during deoxyribonucleic acid synthesis at single strand breaks. J. Biol. Chem., 246, 2692-2701 (1971).
12. Longley, M. J., Bennett, S. E., and Mosbaugh, D. W., Characterization of the 50 to 30 exonuclease associated with Thermus aquaticus DNA polymerase. Nucleic Acids Res., 18, 7317-7322 (1990).
13. Sambrook, J., and Russell, D. W., "Molecular Cloning, a Laboratory Manual" 3rd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor (2001).
14. Sadler, J. R., Sasmor, H., and Betz, J. L., A perfectly symmetric lac operator binds the lac repressor very tightly. Proc. Natl. Acad. Sci. USA, 80, 6785-6789 (1983).
15. Schmitz, A., Cyclic AMP receptor protein interacts with lactose operator DNA. Nucleic Acids Res., 9, 277-292 (1981).