메뉴 건너뛰기




Volumn 83, Issue 1, 2012, Pages 75-83

Recombinant expression, in vitro refolding, and biophysical characterization of the N-terminal domain of T1R3 taste receptor

Author keywords

Bacterial expression; Protein refolding; Recombinant GPCR; Sweetener; Taste receptor

Indexed keywords

DRUG DERIVATIVE; G PROTEIN COUPLED RECEPTOR; RECOMBINANT PROTEIN; SUCROSE; TASTE RECEPTORS, TYPE 1; TRICHLOROSUCROSE;

EID: 84859322059     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2012.03.006     Document Type: Article
Times cited : (37)

References (39)
  • 10
    • 69549111756 scopus 로고    scopus 로고
    • Molecular pharmacology of promiscuous seven transmembrane receptors sensing organic nutrients
    • P. Wellendorph, L.D. Johansen, and H. Bräuner-Osborne Molecular pharmacology of promiscuous seven transmembrane receptors sensing organic nutrients Mol. Pharmacol. 76 2009 453 465
    • (2009) Mol. Pharmacol. , vol.76 , pp. 453-465
    • Wellendorph, P.1    Johansen, L.D.2    Bräuner-Osborne, H.3
  • 14
    • 27644568166 scopus 로고    scopus 로고
    • Distinct contributions of T1R2 and T1R3 taste receptor subunits to the detection of sweet stimuli
    • DOI 10.1016/j.cub.2005.09.037, PII S0960982205011073
    • Y. Nie, S. Vigues, J.R. Hobbs, G.L. Conn, and S.D. Munger Distinct contributions of T1R2 and T1R3 taste receptor subunits to the detection of sweet stimuli Curr. Biol. 15 2005 1948 1952 (Pubitemid 41566143)
    • (2005) Current Biology , vol.15 , Issue.21 , pp. 1948-1952
    • Nie, Y.1    Vigues, S.2    Hobbs, J.R.3    Conn, G.L.4    Munger, S.D.5
  • 15
    • 33751505567 scopus 로고    scopus 로고
    • The heterodimeric sweet taste receptor has multiple potential ligand binding sites
    • DOI 10.2174/138161206779010350
    • M. Cui, P. Jiang, E. Maillet, M. Max, R.F. Margolskee, and R. Osman The heterodimeric sweet taste receptor has multiple potential ligand binding sites Curr. Pharm. Des. 12 2006 4591 4600 (Pubitemid 44835147)
    • (2006) Current Pharmaceutical Design , vol.12 , Issue.35 , pp. 4591-4600
    • Cui, M.1    Jiang, P.2    Maillet, E.3    Max, M.4    Margolskee, R.E.5    Osman, R.6
  • 16
    • 26644458124 scopus 로고    scopus 로고
    • Identification of the cyclamate interaction site within the transmembrane domain of the human sweet taste receptor subunit T1R3
    • DOI 10.1074/jbc.M505255200
    • P. Jiang, M. Cui, B. Zhao, L.A. Snyder, L.M. Benard, R. Osman, M. Max, and R.F. Margolskee Identification of the cyclamate interaction site within the transmembrane domain of the human sweet taste receptor subunit T1R3 J. Biol. Chem. 280 2005 34296 34305 (Pubitemid 41443155)
    • (2005) Journal of Biological Chemistry , vol.280 , Issue.40 , pp. 34296-34305
    • Jiang, P.1    Cui, M.2    Zhao, B.3    Snyder, L.A.4    Benard, L.M.J.5    Osman, R.6    Max, M.7    Margolskee, R.F.8
  • 18
    • 36749099088 scopus 로고    scopus 로고
    • The binding site for neohesperidin dihydrochalcone at the human sweet taste receptor
    • M. Winnig, B. Bufe, N.A. Kratochwil, J.P. Slack, and W. Meyerhof The binding site for neohesperidin dihydrochalcone at the human sweet taste receptor BMC Struct. Biol. 7 2007 66
    • (2007) BMC Struct. Biol. , vol.7 , pp. 66
    • Winnig, M.1    Bufe, B.2    Kratochwil, N.A.3    Slack, J.P.4    Meyerhof, W.5
  • 20
    • 33747865176 scopus 로고    scopus 로고
    • Expression and purification of functional ligand-binding domains of T1R3 taste receptors
    • DOI 10.1093/chemse/bjj053
    • Y. Nie, J.R. Hobbs, S. Vigues, W.J. Olson, G.L. Conn, and S.D. Munger Expression and purification of functional ligand-binding domains of T1R3 taste receptors Chem. Senses 31 2006 505 513 (Pubitemid 44282980)
    • (2006) Chemical Senses , vol.31 , Issue.6 , pp. 505-513
    • Nie, Y.1    Hobbs, J.R.2    Vigues, S.3    Olson, W.J.4    Conn, G.L.5    Munger, S.D.6
  • 21
    • 80053141646 scopus 로고    scopus 로고
    • GFP-based evaluation system of recombinant expression through the secretory pathway in insect cells and its application to the extracellular domains of class C GPCRs
    • Y. Ashikawa, M. Ihara, N. Matsuura, Y. Fukunaga, Y. Kusakabe, and A. Yamashita GFP-based evaluation system of recombinant expression through the secretory pathway in insect cells and its application to the extracellular domains of class C GPCRs Protein Sci. 20 2011 1720 1734
    • (2011) Protein Sci. , vol.20 , pp. 1720-1734
    • Ashikawa, Y.1    Ihara, M.2    Matsuura, N.3    Fukunaga, Y.4    Kusakabe, Y.5    Yamashita, A.6
  • 23
    • 0032789940 scopus 로고    scopus 로고
    • A new protein folding screen: Application to the ligand binding domains of a glutamate and kainate receptor and to lysozyme and carbonic anhydrase
    • N. Armstrong, A. de Lencastre, and E. Gouaux A new protein folding screen: application to the ligand binding domains of a glutamate and kainate receptor and to lysozyme and carbonic anhydrase Protein Sci. 8 1999 1475 1483 (Pubitemid 29328228)
    • (1999) Protein Science , vol.8 , Issue.7 , pp. 1475-1483
    • Armstrong, N.1    De Lencastre, A.2    Gouaux, E.3
  • 24
    • 0024448151 scopus 로고
    • Calculation of protein extinction coefficients from amino acid sequence data
    • S.C. Gill, and P.H. von Hippel Calculation of protein extinction coefficients from amino acid sequence data Anal. Biochem. 182 1989 319 326 (Pubitemid 19277112)
    • (1989) Analytical Biochemistry , vol.182 , Issue.2 , pp. 319-326
    • Gill, S.C.1    Von Hippel, P.H.2
  • 25
    • 45849096536 scopus 로고    scopus 로고
    • Protein secondary structure analyzes from circular dichroism spectroscopy: Methods and reference databases
    • L. Whitmore, and B.A. Wallace Protein secondary structure analyzes from circular dichroism spectroscopy: methods and reference databases Biopolymers 89 2008 392 400
    • (2008) Biopolymers , vol.89 , pp. 392-400
    • Whitmore, L.1    Wallace, B.A.2
  • 26
    • 0345293146 scopus 로고    scopus 로고
    • On the Value of c: Can Low Affinity Systems Be Studied by Isothermal Titration Calorimetry?
    • DOI 10.1021/ja036166s
    • W.B. Turnbull, and A.H. Daranas On the value of c: can low affinity systems be studied by isothermal titration calorimetry? J. Am. Chem. Soc. 125 2003 14859 14866 (Pubitemid 37475576)
    • (2003) Journal of the American Chemical Society , vol.125 , Issue.48 , pp. 14859-14866
    • Turnbull, W.B.1    Daranas, A.H.2
  • 27
    • 22244468386 scopus 로고    scopus 로고
    • Investigation of protein refolding using a fractional factorial screen: A study of reagent effects and interactions
    • DOI 10.1110/ps.051433205
    • M.S. Willis, J.K. Hogan, P. Prabhakar, X. Liu, K. Tsai, Y. Wei, and T. Fox Investigation of protein refolding using a fractional factorial screen: a study of reagent effects and interactions Protein Sci. 14 2005 1818 1826 (Pubitemid 40994152)
    • (2005) Protein Science , vol.14 , Issue.7 , pp. 1818-1826
    • Willis, M.S.1    Hogan, J.K.2    Prabhakar, P.3    Liu, X.4    Tsai, K.5    Wei, Y.6    Fox, T.7
  • 28
    • 0033574418 scopus 로고    scopus 로고
    • Expression, purification and biochemical characterization of the amino-terminal extracellular domain of the human calcium receptor
    • P.K. Goldsmith, G.F. Fan, K. Ray, J. Shiloach, P. McPhie, K.V. Rogers, and A.M. Spiegel Expression, purification and biochemical characterization of the amino-terminal extracellular domain of the human calcium receptor J. Biol. Chem. 274 1999 11303 11309
    • (1999) J. Biol. Chem. , vol.274 , pp. 11303-11309
    • Goldsmith, P.K.1    Fan, G.F.2    Ray, K.3    Shiloach, J.4    McPhie, P.5    Rogers, K.V.6    Spiegel, A.M.7
  • 29
    • 0030955264 scopus 로고    scopus 로고
    • SAmBA: An interactive software for optimizing the design of biological macromolecules crystallization experiments
    • DOI 10.1002/(SICI)1097-0134(199710)29:2<252::AID-PROT12>3.0.CO;2-N
    • S. Audic, F. Lopez, J.M. Claverie, O. Poirot, and C. Abergel SAmBA: an interactive software for optimizing the design of biological macromolecules crystallization experiments Proteins 29 1997 252 257 (Pubitemid 27430939)
    • (1997) Proteins: Structure, Function and Genetics , vol.29 , Issue.2 , pp. 252-257
    • Audic, S.1    Lopez, F.2    Claverie, J.-M.3    Poirot, O.4    Abergel, C.5
  • 30
    • 0031471216 scopus 로고    scopus 로고
    • Overexpression of a glutamate receptor (GluR2) ligand binding domain in Escherichia coli: Application of a novel protein folding screen
    • DOI 10.1073/pnas.94.25.13431
    • G.Q. Chen, and E. Gouaux Overexpression of a glutamate receptor (GluR2) ligand binding domain in Escherichia coli: application of a novel protein folding screen Proc. Natl Acad. Sci. USA 94 1997 13431 13436 (Pubitemid 28009599)
    • (1997) Proceedings of the National Academy of Sciences of the United States of America , vol.94 , Issue.25 , pp. 13431-13436
    • Chen, G.-Q.1    Gouaux, E.2
  • 32
    • 34547925579 scopus 로고    scopus 로고
    • Expression in Escherichia coli, refolding and crystallization of Aspergillus niger feruloyl esterase A using a serial factorial approach
    • DOI 10.1016/j.pep.2007.04.001, PII S1046592807000940
    • I. Benoit, B. Coutard, R. Oubelaid, M. Asther, and C. Bignon Expression in Escherichia coli, refolding and crystallization of Aspergillus niger feruloyl esterase A using a serial factorial approach Protein Expression and Purif. 55 2007 166 174 (Pubitemid 47259684)
    • (2007) Protein Expression and Purification , vol.55 , Issue.1 , pp. 166-174
    • Benoit, I.1    Coutard, B.2    Oubelaid, R.3    Asther, M.4    Bignon, C.5
  • 35
    • 34047237831 scopus 로고    scopus 로고
    • d determined using competition titration calorimetry
    • DOI 10.1021/bi700013y
    • d determined using competition titration calorimetry Biochemistry 46 2007 4017 4027 (Pubitemid 46536065)
    • (2007) Biochemistry , vol.46 , Issue.13 , pp. 4017-4027
    • Tse, J.K.Y.1    Giannetti, A.M.2    Bradshaw, J.M.3
  • 37
    • 0037179658 scopus 로고    scopus 로고
    • General pseudoreceptor model for sweet compounds: A semiquantitative prediction of binding affinity for sweet-tasting molecules
    • A. Bassoli, M.G. Drew, L. Merlini, and G. Morini General pseudoreceptor model for sweet compounds: a semiquantitative prediction of binding affinity for sweet-tasting molecules J. Med. Chem. 45 2002 4402 4409
    • (2002) J. Med. Chem. , vol.45 , pp. 4402-4409
    • Bassoli, A.1    Drew, M.G.2    Merlini, L.3    Morini, G.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.