Effects of substrate feed rates on heterologous protein expression by Pichia pastoris in DO-stat fed-batch fermentation

Enzyme and Microbial Technology

Volumn 33, Issue 4, 2003, Pages 358-365

  Lee, Chun Yeon ^a   Nakano, Atsushi ^a   Shiomi, Naofumi ^b   Lee, Eun Kyu ^c   Katoh, Shigeo ^a  

^a KOBE UNIVERSITY   (Japan)

^b KOBE COLLEGE   (Japan)

^c Hanyang University   (South Korea)

Author keywords

Alpha amylase; DO stat; High cell density fermentation; Induction with methanol; Methylotrophic yeast

Indexed keywords

FERMENTATION; GLYCEROL; METHANOL;

CELL GROWTH;

PROTEINS;

ALCOHOL OXIDASE; AMYLASE; GLYCEROL; METHANOL;

CELL DENSITY; CHIMERA; CONFERENCE PAPER; CONTROLLED STUDY; ENZYME ACTIVITY; FED BATCH FERMENTATION; GROWTH RATE; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION;

PICHIA; PICHIA PASTORIS;

EID: 0042932499     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0141-0229(03)00146-7     Document Type: Conference Paper

References (24)

1. Cregg J.M., Vedvick T.S., Raschke W.C. Recent advances in the expression of foreign genes in Pichia pastoris. Biotechnology. 11:1993;905-910.
2. Ellis S.B., Brust P.F., Koutz P.J., Waters A.F., Harpold M.M., Gineras T.R. Isolation of alcohol oxidase and two other methanol regulatable genes from the yeast Pichia pastoris. Mol. Cell Biol. 5:1985;1111-1121.
3. Veenhuis M., Van Dijken J.P., Harder W. The significance of peroxisomes in the metabolism of one-carbon compounds in yeasts. Adv. Microb. Physiol. 24:1983;1-82.
4. Romanos M. Advances in the use of Pichia pastoris for high-level gene expression. Curr. Opin. Biotechnol. 6:1995;527-533.
5. Wegner G.H. Emerging applications of the methylotrophic yeasts. FEMS Microbiol. Rev. 87:1990;279-284.
6. Cereghino G.P.L., Cereghino J.L., Ilgen C., Cregg J.M. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr. Opin. Biotechnol. 13:2002;1-4.
7. Couderc R., Barratti J. Oxidation of methanol by the yeast Pichia pastoris: purification and properties of alcohol oxidase. Agric. Biol. Chem. 44:1980;2279-2289.
8. Murray W.D., Duff S.J.B., Lauthier P.H. Induction and stability of alcohol oxidase in the methylotrophic yeast Pichia pastoris. Appl. Microbiol. Biotechnol. 32:1989;95-100.
9. Stratton J, Chiruvolu V, Meagher M. High-cell-density fermentation. In: Higgins DR, Cregg JM, editors. Method in molecular biology, vol. 103. Totowa, NJ: Humana Press; 1998. p. 107-20.
10. Zhang W.H., Inan M., Meagher M.M. Fermentation strategies for recombinant protein expression in the methylotrophic yeast Pichia pastoris. Biotechnol. Bioprocess. Eng. 5:2000;275-287.
11. Chen Y.L., Cino J., Hart G., Feedman D., White C., Komives E.A. High protein expression in fermentation of recombinant Pichia pastoris by a fed-batch process. Process Biochem. 32:1997;107-111.
12. Chung J.D. Design of metabolic feed controllers: application to high-density fermentations of Pichia pastoris. Biotech. Bioeng. 68:2000;298-307.
13. Brierley R.A., Bussineau C., Kosson R., Melton A., Siegel R.S. Fermentation development of recombinant Pichia pastoris expressing the heterologous gene: bovine lysozyme. Ann. N.Y. Acad. Sci. 589:1990;350-362.
14. Zhang W.H., Bevins M.A., Plantz B.A., Smith L.A., Meagher M.M. Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotech. Bioeng. 70:2000;1-8.
15. Lee J.S., Lee S.Y., Park S.W., Middelberg A.P.J. Control of fed-batch fermentations. Biotechnol. Adv. 17:1999;29-48.
16. Steup M. Starch degradation. In: Preiss J, editor. The biochemistry of plants, vol. 14. San Diego, CA: Academic Press; 1988. p. 255-96.
17. Terashima M., Kawai M., Kumagai M.H., Rodriguez R.L., Katoh S. Characteristics of a chimeric enzyme engineered from two rice α-amylase isozymes. Appl. Microbiol. Biotechnol. 45:1996;607-611.
18. Lee C.Y., Lee S.J., Jung K.H., Katoh S., Lee E.K. High dissolved oxygen tension enhances heterologous protein expression by recombinant Pichia pastoris. Process Biochem. 38:2003;1147-1154.
19. Kumagai M.H., Shaw M., Terashima M., Vrkljan Z., Whitaker J.R., Rodriguez R.L. Expression and secretion of rice α-amylase by Saccharomyces cerevisiae. Gene. 94:1990;209-216.
20. Kurokawa T., Lee C.Y., Shiomi N., Nakano A., Katoh S. Secretion of α-amylase from Saccharomyces cerevisiae and Pichia pastoris and characterization of its C-terminus with an anti-peptide antibody. J. Chem. Eng. Jpn. 35:2002;1277-1281.
21. Bernfeld B. Amylases α and β. Methods enzymol. San Diego, CA: Academic Press; 1955. p. 149-58.
22. Katoh S., Terashima M., Miyaoku K. Purification of α-amylase by specific elution from anti-peptide antibodies. Appl. Microbiol. Biotechnol. 47:1997;521-524.
23. Inan M., Chiruvolu V., Eskridge K.M., Vlasuk G.P., Dickerson K., Brown S.et al. Optimization of temperature glycerol pH conditions for a fed-batch fermentation process for recombinant hookworm (Ancylostoma caninum) anticoagulant peptide (AcAP-5) production by Pichia pastoris. Enzyme Microb. Technol. 24:1999;438-445.
24. Inan M., Meagher M.M. Non-repressing carbon sources for alcohol oxidase (AOX1) promoter of Pichia pastoris. J. Biosci. Bioeng. 92:2001;585-589.