Production of recombinant porcine interferon alpha using PHB-intein-mediated protein purification strategy

Applied Biochemistry and Biotechnology

Volumn 163, Issue 8, 2011, Pages 981-993

  Zhou, Xuezhang ^a   Song, Zhenwei ^a,b   Liu, Xiaoming ^a   Jia, Fang ^a   Wang, Yujiong ^a  

^a NINGXIA UNIVERSITY   (China)

^b SUN YAT SEN UNIVERSITY   (China)

Author keywords

IFN alpha; Intein; Poly hydroxybutyrate; Porcine; Purification

Indexed keywords

AFFINITY TAG; ANTI-INFECTIOUS AGENTS; ANTIVIRAL ACTIVITIES; BINDING PROTEINS; CULTURE MEDIUM; DOMESTIC ANIMALS; ESCHERICHIA COLI CELLS; EXPERIMENTAL STUDIES; HIGH YIELD; IFN ALPHA; INFECTIOUS DISEASE; INTEIN; LARGE-SCALE PRODUCTION; POLYHYDROXYBUTYRATE; PORCINE; PROTEIN EXPRESSIONS; PROTEIN PURIFICATION; SODIUM LACTATE;

ANIMALS; ANTIBIOTICS; CELL CULTURE; DISEASE CONTROL; DISEASES; ESCHERICHIA COLI; GLYCOPROTEINS; INTERFERONS; LIQUID CHROMATOGRAPHY; SODIUM;

PURIFICATION;

HYDROXYBUTYRIC ACID; INTEIN; POLYHYDROXYBUTYRATE; RECOMBINANT ALPHA INTERFERON; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIUM CULTURE; BINDING AFFINITY; ESCHERICHIA COLI; EXPERIMENTAL PIG; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PROTEIN EXPRESSION; PROTEIN PURIFICATION;

ANIMALS; CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; INTEINS; INTERFERON-ALPHA; POLYMERS; PROTEIN ENGINEERING; PROTEIN SPLICING; RECOMBINANT FUSION PROTEINS; SWINE;

ANIMALIA; ESCHERICHIA COLI; SUIDAE; SUS;

EID: 79952656160     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-010-9101-2     Document Type: Article

References (30)

1. Banki, M. R., Gerngross, T. U., & Wood, D. W. (2005). Novel and economical purification of recombinant proteins: Intein-mediated protein purification using in vivo polyhydroxybutyrate (PHB) matrix association. Protein Science, 14, 1387-1395. (Pubitemid 41007905)
2. Barbe, F., Saelens, X., Braeckmans, D., Lefevre, F., & Reeth, K. V. (2010). Role of IFN-alpha during the acute stage of a swine influenza virus infection. Research in Veterinary Science, 88, 172-178.
3. Berg, K., Hansen, M. B., & Nielsen, S. E. (1990). A new sensitive bioassay for precise quantification of interferon activity as measured via the mitochondrial dehydrogenase function in cells (MTT-method). APMIS, 98, 156-162.
4. Buddaert, W., Van Reeth, K., & Pensaert, M. (1998). In vivo and in vitro interferon (IFN) studies with the porcine reproductive and respiratory syndrome virus (PRRSV). Advances in Experimental Medicine and Biology, 440, 461-467.
5. Candiano,G., Bruschi,M.,Musante,L., Santucci, L.,Ghiggeri, G. M.,Carnemolla, B., et al. (2004).Blue silver: Avery sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis, 25, 1327-1333.
6. Diaz-San Segundo, F., Moraes, M. P., de Los Santos, T., Dias, C. C., & Grubman, M. J. (2010). Interferon-induced protection against foot-and-mouth disease virus infection correlates with enhanced tissue-specific innate immune cell infiltration and interferon-stimulated gene expression. Journal of Virology, 84, 2063-2077.
7. Du, Y., Dai, J., Li, Y., Li, C., Qi, J., Duan, S., et al. (2008). Immune responses of recombinant adenovirus co-expressing VP1 of foot-and-mouth disease virus and porcine interferon alpha in mice and guinea pigs. Veterinary Immunology and Immunopathology, 124, 274-283.
8. Georgiou, G., & Jeong, K. J. (2005). Proteins from PHB granules. Protein Science, 14, 1385-1386. (Pubitemid 41007904)
9. Gillies, A. R., Mahmoud, R. B., & Wood, D. W. (2009). PHB-intein-mediated protein purification strategy. Methods in Molecular Biology, 498, 173-183.
10. Grantham, R., Gautier, C., Gouy, M., Jacobzone, M., & Mercier, R. (1981). Codon catalog usage is a genome strategy modulated for gene expressivity. Nucleic Acids Research, 9, r43-r74. (Pubitemid 11128643)
11. Grantham, R., Gautier, C., Gouy, M., Mercier, R., & Pave, A. (1980). Codon catalog usage and the genome hypothesis. Nucleic Acids Research, 8, r49-r62. (Pubitemid 10154683)
12. Hanada, N., Morishima, T., Nishikawa, K., Isomura, S.,& Nagai, Y. (1986). Interferon-mediated self-limiting growth of respiratory syncytial virus in mouse embryo cells. Journal of Medical Virology, 20, 363-370.
13. Horisberger, M. A. (1992). Virus-specific effects of recombinant porcine interferon-gamma and the induction of Mx proteins in pig cells. Journal of Interferon Research, 12, 439-444.
14. Jordan, L. T., & Derbyshire, J. B. (1995). Antiviral action of interferon-alpha against porcine transmissible gastroenteritis virus. Veterinary Microbiology, 45, 59-70.
15. La Bonnardiere, C., Lefevre, F., & Charley, B. (1994). Interferon response in pigs: Molecular and biological aspects. Veterinary Immunology and Immunopathology, 43, 29-36. (Pubitemid 24327525)
16. Lefevre, F., L'Haridon, R., Borras-Cuesta, F., & La Bonnardiere, C. (1990). Production, purification and biological properties of an Escherichia coli-derived recombinant porcine alpha interferon. The Journal of General Virology, 71(Pt 5), 1057-1063. (Pubitemid 20174344)
17. Lefevre, F., & La Bonnardiere, C. (1986). Molecular cloning and sequencing of a gene encoding biologically active porcine alpha-interferon. Journal of Interferon Research, 6, 349-360. (Pubitemid 16003255)
18. Lefevre, F., Mege, D., L'Haridon, R., Bernard, S., De Vaureix, C., & La Bonnardiere, C. (1990). Contribution of molecular biology to the study of the porcine interferon system. Veterinary Microbiology, 23, 245-257. (Pubitemid 20299909)
19. Mach, H., Middaugh, C. R., & Denslow, N. (2001) Determining the identity and purity of recombinant proteins by UV absorption spectroscopy. Curr Protoc Protein Sci, Chapter 7, Unit 7 2.
20. Meager, A., Gaines Das, R., Zoon, K., & Mire-Sluis, A. (2001). Establishment of new and replacement World Health Organization International Biological Standards for human interferon alpha and omega. Journal of Immunological Methods, 257, 17-33.
21. Nfon, C. K., Ferman, G. S., Toka, F. N., Gregg, D. A., & Golde, W. T. (2008). Interferon-alpha production by swine dendritic cells is inhibited during acute infection with foot-and-mouth disease virus. Viral Immunology, 21, 68-77. (Pubitemid 351450458)
22. Osterlund, P., Pirhonen, J., Ikonen, N., Ronkko, E., Strengell, M., Makela, S. M., et al. (2010). Pandemic H1N1 2009 influenza A virus induces weak cytokine responses in human macrophages and dendritic cells and is highly sensitive to the antiviral actions of interferons. Journal of Virology, 84, 1414-1422.
23. Peroza, E. A., & Freisinger, E. (2008). Tris is a non-innocent buffer during intein-mediated protein cleavage. Protein Expression and Purification, 57, 217-225. (Pubitemid 350296977)
24. Platanias, L. C. (2005). Mechanisms of type-I- and type-II-interferon- mediated signalling. Nature Reviews. Immunology, 5, 375-386.
25. Pol, J. M. A., Broekhuysen-Davies, J. M., Wagenaar, F., & La Bonnardiere, C. (1991). The influence of porcine recombinant interferon-alpha 1 on pseudorabies virus infection of porcine nasal mucosa in vitro. The Journal of General Virology, 72(Pt 4), 933-938.
26. Ramagli, L. S. (1999). Quantifying protein in 2-D page solubilization buffers. In A. Link (Ed.), Methods in molecular biology (Vol. 112, pp. 99-103). Totowa: Humana.
27. Stauffer, F., De Miranda, J., Schechter, M. C., Carneiro, F. A., Salgado, L. T., Machado, G. F., et al. (2007). Inactivation of vesicular stomatitis virus through inhibition of membrane fusion by chemical modification of the viral glycoprotein. Antiviral Research, 73, 31-39. (Pubitemid 46080477)
28. Terpe, K. (2003). Overview of tag protein fusions: From molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60, 523-533.
29. Woo, P. C., Tung, E. T., Chan, K. H., Lau, C. C., Lau, S. K., & Yuen, K. Y. (2010). Cytokine profiles induced by the novel swine-origin influenza A/H1N1 virus: Implications for treatment strategies. The Journal of Infectious Diseases, 201, 346-353.
30. Xiong, Y., Lin, M., Yuan, B., Yuan, T., & Zheng, C. (2009). Expression of exogenous IFN-alpha by bypassing the translation block protects cells against FMDV infection. Antiviral Research, 84, 60-66.