In vitro phenotypic, genomic and proteomic characterization of a cytokine-resistant murine β-TC3 cell line

PLoS ONE

Volumn 7, Issue 2, 2012, Pages

  Coppola, Antonina ^a   Tomasello, Laura ^a   Pizzolanti, Giuseppe ^a   Pucci Minafra, Ida ^a   Albanese, Nadia ^a   Di Cara, Gianluca ^a   Cancemi, Patrizia ^a   Pitrone, Maria ^a   Bommarito, Alessandra ^a   Carissimi, Elvira ^a   Zito, Giovanni ^a   Criscimanna, Angela ^a   Galluzzo, Aldo ^a   Giordano, Carla ^a,b  

^a UNIVERSITY OF PALERMO   (Italy)

^b CNR   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MARKER; GAMMA INTERFERON; GAMMA INTERFERON RECEPTOR; GLUCOSE TRANSPORTER 2; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INSULIN; INTERLEUKIN 1BETA; JANUS KINASE; POTASSIUM CHLORIDE; STAT PROTEIN; SUMO PROTEIN; SUMO4 PROTEIN; SUPPRESSOR OF CYTOKINE SIGNALING 3; TOLBUTAMIDE; TRANSCRIPTION FACTOR NKX6.1; TRANSCRIPTION FACTOR PDX 1; UNCLASSIFIED DRUG; CYTOKINE;

ANIMAL CELL; ARTICLE; B LYMPHOCYTE; CELL DEATH; CELL LINE; CELL SECRETION; CELL STRESS; CONTROLLED STUDY; DOWN REGULATION; ENZYME INHIBITION; FEEDBACK SYSTEM; GENE EXPRESSION REGULATION; GENE SILENCING; GENOMICS; IMMUNITY; INSULINOMA; LYMPHOCYTE PROLIFERATION; MOUSE; NONHUMAN; PHENOTYPE; PROTEIN DEFECT; PROTEIN FOLDING; PROTEOMICS; SECRETORY CELL; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; UPREGULATION; ANIMAL; APOPTOSIS; CELL CYCLE; CELL PROLIFERATION; CELL SURVIVAL; CULTURE TECHNIQUE; CYTOLOGY; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; METABOLISM; METHODOLOGY; PANCREAS ISLET BETA CELL;

MURINAE;

ANIMALS; APOPTOSIS; CELL CULTURE TECHNIQUES; CELL CYCLE; CELL DEATH; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; CYTOKINES; DOWN-REGULATION; GENE SILENCING; GENOMICS; IMMUNOHISTOCHEMISTRY; INSULIN-SECRETING CELLS; INSULINOMA; MICE; NF-KAPPA B; PHENOTYPE; PROTEOMICS;

EID: 84857710334     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0032109     Document Type: Article

References (45)

1. Kaminitz A, Stein J, Yaniv I, Askenasy N, (2007) The vicious cycle of apoptotic β-cell death in type1diabetes. Immunol Cell Biol 85: 582-589.
2. Watson D, Loweth AC, (2009) Oxidative and nitrosative stress in β-cell apoptosis: their contribution to β-cell loss in type 1 diabetes mellitus. Br J Biomed Sci 66: 208-215.
3. Eizirik DL, Colli ML, Ortis F, (2009) The role of inflammation in insulitis and β-cell loss in type 1 diabetes. Nat Rev Endocrinol 5: 219-226 Review.
4. EizriK DL, Mandrup Poulsen T, (2001) A choice of death- the signal-transduction of immune- mediated beta-cell apoptosis. Diabetologia 44: 2115-2133.
5. Stassi G, Todaro M, Richiusa P, Giordano M, Mattina A, et al. (1995) Expression of apoptosis-inducing CD95 (Fas/Apo-1) on human β-cells sorted by flow-cytometry and cultured in vitro. Transplant Proc 27: 3271-3275.
6. Melloul D, (2008) Role of NF-kB in β-cell death. Biochem Soc Trans 36: 334-339.
7. Suk K, Kim S, Kim YH, Kim KA, Chang I, et al. (2001) IFN-γ/TNF-α synergism as the final effector in autoimmune diabetes: a key role for STAT-1/IFN regulatory factor-1 pathway in pancreatic β-cell death. J Immunol 166: 4481-4489.
8. Cardozo AK, Heimberg H, Heremans Y, Leeman R, Kutlu B, et al. (2001) A comprehensive analysis of cytokine-induced and nuclear factor-κB-dependent genes in primary rat pancreatic β-cells. J Biol Chem 276: 48879-48886.
9. Ortis F, Cardozo AK, Crispim Det Störling J, Mandrup-Poulsen T, et al. (2006) Cytokine-induced proapoptotic gene expression in insulin-producing cells is related to rapid, sustained, and nonoscillatory nuclear factor-κB activation. Mol Endocrinol 20: 1867-1879.
10. Nicoletti F, Di Marco R, Barcellini W, Magro G, Schorlemmer HU, et al. (1994) Protection from experimental autoimmune diabetes in the non-obese diabetic mouse with soluble interleukin-1 receptor. Eur J Immunol 24: 1843-1847.
11. Cailleau C, Diu-Hercend A, Ruuth E, Westwood R, Carnaud C, (1997) Treatment with neutralizing antibodies specific for IL-1 beta prevents cyclophosphamide-induced diabetes in nonobese diabetic mice. Diabetes 46: 937-940.
12. Nicoletti F, Zaccone P, Dimarco R, Lunetta M, Magro G, et al. (1997) Prevention of spontaneous autoimmune diabetes in diabetes-prone BB rats by prophylactic treatment with antirat interferon-γ antibody. Endocrinology 138: 281-288.
13. Nielsen K, Sparre T, Larsen MR, Nielsen M, Fey SJ, et al. (2004) Protein expression changes in a cell system of beta-cell maturation reflect an acquired sensitivity to IL-1β. Diabetologia 47: 62-74.
14. Welsh N, Eizirik DL, Bendtzen K, Sandler S, (1991) Interleukin-1β-induced nitric oxide production in isolated rat pancreatic islets requires gene transcription and may lead to inhibition of the Krebs cycle enzyme aconitase. Endocrinology 129: 3167-3173.
15. Southern C, Schulster D, Green IC, (1990) Inhibition of insulin secretion from rat islets of Langerhans by interleukin-6. An effect distinct from that of interleukin-1. Biochem J 15;272: 243-245.
16. Sjöholm A, (1991) Cytokines inhibit proliferation and insulin secretion by clonal rat insulinoma cells (RINm5F) non-synergistically and in a pertussis toxin-insensitive manner. Immunol Lett 30: 81-86.
17. Efrat S, Lindet S, Kofodt H, Spector D, Delannoy M, et al. (1988) Beta-cell lines derived from transgenic mice expressing a hybrid insulin gene oncogene. Proc Natl Acad Sci U S A 85: 9037-9041.
18. Pucci-Minafra I, Fontana S, Cancemi P, Basiricò L, Caricato S, et al. (2002) A contribution to breast cancer cell proteomics: detection of new sequences. Proteomics 2: 919-927.
19. Dinarello CA, (1998) Interleukin-1, interleukin-1 receptors and interleukin-1 receptor antagonist. Int Rev Immunol 16: 457-499.
20. Karlsen AE, Rønn SG, Lindberg K, Johannesen J, Galsgaard ED, et al. (2001) Suppressor of cytokine signaling 3 (SOCS-3) protects β-cells against interleukin-1β- and interferon-γ-mediated toxicity. Proc Natl Acad Sci U S A 98: 12191-12196.
21. Ahmed M, Bergsten P, (2005) Glucose-induced changes of multiple mouse islet proteins analysed by two-dimensional gel electrophoresis and mass spectrometry. Diabetologia 48: 477-485.
22. Fernandez C, Fransson U, Hallgard E, Spégel P, Holm C, et al. (2008) Metabolomic and proteomic analysis of a clonal insulin-producing β-cell line (INS-1 832/13). J Proteome Res 7: 400-411.
23. Larsen PM, Fey SJ, Larsen MR, Nawrocki A, Andersen HU, et al. (2001) Proteome analysis of interleukin-1β-induced changes in protein expression in rat islets of Langerhans. Diabetes 50: 1056-1063.
24. Sparre T, Christensen UB, Mose Larsen P, Fey SJ, Wrzesinski K, et al. (2002) IL-1β-induced protein changes in diabetes prone BB rat islets of Langerhans identified by proteome analysis. Diabetologia 45: 1550-1561.
25. D'Hertog W, Overbergh L, Lage K, Ferreira GB, Maris M, et al. (2007) Proteomics analysis of cytokine-induced dysfunction and death in insulin-producing INS-1E cells. Mol Cell Proteomics 6: 2180-2199.
26. Johnson JD, Bernal-Mizrachi E, Alejandro EU, Han Z, Kalynyak TB, et al. (2006) Insulin protects islets from apoptosis via Pdx1 and specific changes in the human islet proteome. Proc Natl Acad Sci U S A 103: 19575-19580.
27. Minami K, Yano H, Miki T, Nagashima K, Wang CZ, et al. (2000) Insulin secretion and differential gene expression in glucose-responsive and -unresponsive MIN6 sublines. Am J Physiol Endocrinol Metab 279: E773-781.
28. Vionnet N, Stoffel M, Takeda J, Yasuda K, Bell GI, et al. (1992) Nonsense mutation in the glucokinase gene causes early-onset non-insulin-dependent diabetes mellitus. Nature 356: 721-722.
29. Mandrup-Poulsen T, (2001) beta-cell apoptosis: stimuli and signaling. Diabetes 50 (Suppl 1): S58-63.
30. Darville MI, Eizirik DL, (1998) Regulation by cytokines of the inducible nitric oxide synthase promoter in insulin-producing cells. Diabetologia 41: 1101-1108.
31. Pirot P, Eizirik DL, Cardozo AK, (2006) Interferon- γ potentiates endoplasmic reticulum stress-induced death by reducing pancreatic beta cell defence mechanisms. Diabetologia 49: 1229-1236.
32. Wu J, Kaufman RJ, (2006) From acute ER stress to physiological roles of the Unfolded Protein Response. Cell Death Differ 13: 374-384.
33. Whittle N, Li L, Chen WQ, Yang JW, Sartori SB, et al. (2011) Changes in brain protein expression are linked to magnesium restriction-induced depression-like behaviour. Amino Acids 40: 1231-1248.
34. Chen XL, Dodd G, Thomas S, Zhang X, Wasserman MA, et al. (2006) Activation of Nrf2/ARE pathway protects endothelial cells from oxidant injury and inhibits inflammatory gene expression. Am J Physiol Heart Circ Physiol 290: H1862-H1870.
35. Kutlu B, Cardozo AK, Darville MI, Kruhøffer M, Magnusson N, et al. (2003) Discovery of gene networks regulating cytokine-induced dysfunction and apoptosis in insulin-producing INS-1 cells. Diabetes 52: 2701-2719.
36. Cardozo AK, Berthou L, Kruhøffer M, Orntoft T, Nicolls MR, et al. (2003) Gene microarray study corroborates proteomic findings in rodent islet cells. J Proteome Res 2: 553-555.
37. Babic I, Cherry E, Fujita DJ, (2006) SUMO modification of Sam68 enhances its ability to repress cyclin D1 expression and inhibits its ability to induce apoptosis. Oncogene 25: 4955-4964.
38. Wang CY, She JX, (2008) SUMO4 and its role in type 1 diabetes pathogenesis. Diabetes Metab Res Rev 24: 93-102.
39. Kracklauer MP, Schmidt C, (2003) At the crossroads of SUMO and NF-κB. Mol Cancer 2: 39.
40. Wang CY, Yang P, Li M, Gong F, (2009) Characterization of a negative feedback network between SUMO4 expression and NFκB transcriptional activity. Biochem Biophys Res Commun 381: 477-481.
41. Jacobsen ML, Rønn SG, Bruun C, Larsen CM, Eizirik DL, et al. (2009) IL-1β-induced chemokine and Fas expression are inhibited by suppressor of cytokine signalling-3 in insulin-producing cells. Diabetologia 52: 281-288.
42. Stassi G, De Maria R, Trucco G, Rudert W, Testi R, et al. (1997) Nitric oxide primes pancreatic β cells for Fas-mediated destruction in insulin-dependent diabetes mellitus. J Exp Med 186: 1193-1200.
43. Chen G, Hohmeier HE, Newgard CB, (2001) Expression of the transcription factor STAT-1α in insulinoma cells protects against cytotoxic effects of multiple cytokines. J Biol Chem 276: 766-772.
44. Guo D, Li M, Zhang Y, Yang P, Eckenrode S, et al. (2004) A functional variant of SUMO4, a new IκBα modifier, is associated with type 1 diabetes. Nature Genetics 36: 837-841.
45. Zou YF, Feng XL, Tao JH, Zhu JM, Pan FM, et al. (2010) Association of SUMO4 M55V polymorphism with susceptibility to autoimmune and inflammatory diseases: a meta-analysis. Int J Immunogenet 37: 345-354.