Production of bioactive sheep β-defensin-1 in Pichia pastoris

Journal of Industrial Microbiology and Biotechnology

Volumn 39, Issue 1, 2012, Pages 11-17

  Zhao, Pengwei ^a   Cao, Guifang ^a  

^a Veterinary College of Inner Mongolia Agricultural University   (China)

Author keywords

Bioactive; Pichia pastoris; Sheep defensin 1

Indexed keywords

BETA DEFENSIN 1; CHEMOATTRACTANT; HISTIDINE;

ANTIMICROBIAL ACTIVITY; ARTICLE; CARBOXY TERMINAL SEQUENCE; ESCHERICHIA COLI; NONHUMAN; PICHIA PASTORIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEUS VULGARIS; PSEUDOMONAS AERUGINOSA; SHIGELLA FLEXNERI; STAPHYLOCOCCUS AUREUS;

AMINO ACID SEQUENCE; ANIMALS; ANTI-INFECTIVE AGENTS; BETA-DEFENSINS; FUNGI; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; MOLECULAR SEQUENCE DATA; PICHIA; SHEEP;

ESCHERICHIA COLI; FUNGI; NEGIBACTERIA; OVIS; OVIS ARIES; PICHIA PASTORIS; POSIBACTERIA; PROTEUS VULGARIS; PSEUDOMONAS AERUGINOSA; SHIGELLA; STAPHYLOCOCCUS AUREUS;

EID: 84857555225     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-011-0992-x     Document Type: Article

References (23)

1. Bohling A, Hagge SO, Roes S, Podschun R, Sahly H, Harder J, Schroder JM, Grotzinger J, Seydel U, Gutsmann T (2006) LipidspeciWc membrane activity of human beta-defensin-3. Biochemistry 45(17):5663-5670 (Pubitemid 43673196)
2. Brogden KA, Ackermann M, McCray PB, Tack BF (2003) Antimicrobial peptides in animals and their role in host defences. Int J Antimicrob Ag 22(5):465-478 (Pubitemid 37356946)
3. Cabral KMS, Almeida MS, Valente AP, Almeida FCL, Kurtenbach E (2003) Production of the active antifungal Pisum sativum defensin 1 (Psd1) in Pichia pastoris: overcoming the ineYciency of the STE13 protease. Protein Expres Purif 31(1):115-122 (Pubitemid 37207649)
4. Chen HQ, Xu ZN, Peng L, Fang XM, Yin XF, Xu NZ, Cen PL (2006) Recent advances in the research and development of human defensins. Peptides 27(4):931-940
5. CunliVe RN, Mahida YR (2004) Expression and regulation of antimicrobial peptides in the gastrointestinal tract. J Leukocyte Biol 75(1):49-58 (Pubitemid 38166779)
6. Ganz T (2003) Defensins: antimicrobial peptides of innate immunity. Nat Rev Immunol 3(9):710-720 (Pubitemid 41070812)
7. Ganz T (2004) Defensins: antimicrobial peptides of vertebrates. C R Biol 327(6):539-549 (Pubitemid 38979504)
8. Ganz T (2005) Defensins and other antimicrobial peptides: a historical perspective and an update. Comb Chem High Throughput Screen 8(3):209-217 (Pubitemid 40488601)
9. Ganz T, Lehrer RI (1998) Antimicrobial peptides of vertebrates. Curr Opin Immunol 10(1):41-44 (Pubitemid 28112077)
10. Ghosh D, Porter E, Shen B, Lee SK, Wilk D, Drazba J, Yadav SP, Crabb JW, Ganz T, Bevins CL (2002) Paneth cell trypsin is the processing enzyme for human defensin-5. Nat Immunol 3(6):583-590 (Pubitemid 34662312)
11. Gurramkonda C, Adnan A, Gabel T, Lunsdorf H, Ross A, Nemani SK, Swaminathan S, Khanna N, Rinas U (2009) Simple high-cell density fed-batch technique for high-level recombinant protein production with Pichia pastoris: application to intracellular production of hepatitis B surface antigen. Microb Cell Fact 8
12. Hu SY, Li LW, Qiao JJ, Guo YJ, Cheng LS, Liu J (2006) Codon optimization, expression, and characterization of an internalizing anti-ErbB2 single-chain antibody in Pichia pastoris. Protein Expres Purif 47(1):249-257
13. Huttner KM, Brezinski-Caliguri DJ, Mahoney MM, Diamond G (1998) Antimicrobial peptide expression is developmentally regulated in the ovine gastrointestinal tract. J Nutr 128(2):297s-299s (Pubitemid 28090365)
14. Kang HK, Park JY, Ahn JS, Kim SH, Kim D (2009) Cloning of a gene encoding dextranase from Lipomyces starkeyi and its expression in Pichia pastoris. J Microbiol Biotechn 19(2):172-177
15. Lehrer RI, Ganz T (2002) Defensins of vertebrate animals. Curr Opin Immunol 14(1):96-102 (Pubitemid 34085112)
16. Luenser K, Fickel J, Ludwig A (2005) Evolution of caprine and ovine beta-defensin genes. Immunogenetics 57(7):487-498 (Pubitemid 41397188)
17. Maxwell AI, Morrison GM, Dorin JR (2003) Rapid sequence divergence in mammalian beta-defensins by adaptive evolution. Mol Immunol 40(7):413-421 (Pubitemid 37244034)
18. Mi-Jin K, Kim SH, Lee JH, Seo JH, Lee JH, Kim JH, Kim YH, Nam SW (2008) High-level secretory expression of human procarboxypeptidase B by fed-batch cultivation of Pichia pastoris and its partial characterization. J Microbiol Biotechn 18(12):1938-1944
19. Ouellette AJ (2006) Paneth cell alpha-defensin synthesis and function. Curr Top Microbiol 306:1-25 (Pubitemid 47400316)
20. Shi XZ, Karkut T, Chamankhah M, Alting-Mees M, Hemmingsen SM, Hegedus D (2003) Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris. Protein Expres Purif 28(2):321-330 (Pubitemid 36562423)
21. Taylor K, Clarke DJ, McCullough B, Chin W, Seo E, Yang D, Oppenheim J, Uhrin D, Govan JRW, Campopiano DJ, MacMillan D, Barran P, Dorin JR (2008) Analysis and separation of residues important for the chemoattractant and antimicrobial activities of beta-defensin 3. J Biol Chem 283(11):6631-6639
22. Walker JM (2002) The protein protocols handbook, 2nd edn. Humana Press, Totowa, NJ
23. Zhang WH, Bevins MA, Plantz BA, Smith LA, Meagher MM (2000) Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol Bioeng 70(1):1-8