Cloning of a gene encoding dextranase from Lipomyces starkeyi and its expression in Pichia pastoris

Journal of Microbiology and Biotechnology

Volumn 19, Issue 2, 2009, Pages 172-177

  Kang, Hee Kyoung ^a   Park, Ji Young ^b   Ahn, Joon Seob ^c   Kim, Seung Heuk ^d   Kim, Doman ^a  

^a Chonnam National University   (South Korea)

^b Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^c Gang Jin City Agricultural Technology Extension Center   (South Korea)

^d Lifenza Co Ltd   (South Korea)

Author keywords

Dextran; Dextranase; Expression; Lipomyces starkeyi; Pichia pastoris

Indexed keywords

AMINO ACID; DEXTRANASE; METHANOL; MONOCLONAL ANTIBODY; SYNTHETIC PEPTIDE;

ARTICLE; BASE PAIRING; CONTROLLED STUDY; CULTURE MEDIUM; FUNGUS CULTURE; GENE EXPRESSION; GENE SEQUENCE; GENETIC TRANSFORMATION; LIPOMYCES STARKEYI; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; SACCHAROMYCES CEREVISIAE; WESTERN BLOTTING; YEAST;

CLONING, MOLECULAR; DEXTRANASE; FERMENTATION; FUNGAL PROTEINS; GENE EXPRESSION REGULATION, FUNGAL; LIPOMYCES; PICHIA; RECOMBINANT PROTEINS;

LIPOMYCES STARKEYI; ORYCTOLAGUS CUNICULUS; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 64549136727     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0802.100     Document Type: Article

References (27)

1. Barrett, J. F., T. A. Barrett, and R. Curtiss, III. 1987. Purification and partial characterization of the multicomponent dextranase complex of Streptococcus sobrinus and the cloning of the dextranase gene. Infect. Immun. 55: 729-802.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
3. Cereghino, G. P. L., J. L. Cereghino, C. Ilgen, and J. M. Cregg. 2002. Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris. Curr. Opin. Biotechnol. 13: 323-332.
4. Cereghino, J. L. and J. M. Cregg. 2000. Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol. Rev. 24: 45-66.
5. Chaplin, M. F. 1986. Monosaccharides, pp. 1-36. In M. F. Chaplin and J. F. Kennedy (eds.), Carbohydrate Analysis. A Practical Approach. IRL Press, Oxford.
6. Fox, J. D. and J. F. Robyt. 1991. Miniaturization of three carbohydrate analysis using a microsample plate reader. Anal. Biochem. 195: 93-96.
7. Garcia, B., E. Margolles, H. Roca, D. Mateu, M. Raices, M. E. Gonzales, L. Herrera, and J. Delgado. 1996. Cloning and sequencing of a dextranase-encoding cDNA from Penicillum miniolutum. FEMS Microbiol. Lett. 143: 175-183.
8. Jung, Y. U., J. S. Yoo, Y. S. Lee, I. H. Park, S. H. Kim, S. C. Lee, M. Yasuda, S. Y. Chung, and Y. L. Choi. 2007. Purification and characterization of thermostable β-1,3-1,4 glucanase from Bacillus sp. A8-8. Biotechnol. Bioprocess Eng. 12: 265-270.
9. Kang, H. K., S. H. Kim, J. Y. Park, X. J. Jin, D. K. Oh, S. S. Kang, and D. Kim. 2005. Cloning and characterization of a dextranase gene from Lipomyces starkeyi and its expression in Saccharomyces cerevisiae. Yeast 22: 1239-1248.
10. Kim, D. and D. F. Day. 1994. A new process for the production of clinical dextran by mixed-culture fermentation of Lipomyces starkeyi and Leuconostoc mesenteroides. Enzyme Microbiol. Technol. 16: 844-848.
11. Kim, D. and D. F. Day. 1995. Isolation of a dextranase constitutive mutant Lipomyces starkeyi and its use for the production of clinical size dextran. Lett. Appl. Microbiol. 20: 268-270.
12. Kim, D., S. J. Ryu, S. J. Heo, D. W. Kim, and H. S. Kim. 1999. Characterization of a novel carbohydrase from Lipomyces starkeyi KSM 22 for dental application. J. Microbiol. Biotechnol. 9: 260-264.
13. Koenig, D. W. and D. F. Day. 1981. Induction of Lipomyces starkeyi dextranase. Appl. Environ. Microbiol. 55: 2079-2081.
14. Koenig, D. W. and D. F. Day. 1989. The purification and characterization of a dextranase from Lipomyces starkeyi. Eur. J. Biochem. 15: 161-167.
15. Kubo, S., H. Kubota, Y. Ohnishi, T. Morita, T. Matsuta, and A. Matsushiro. 1993. Expression and secretion of an Arthrobacter dextranase in the oral bacterium Streptococcus gordonii. Infect. Immun. 61: 4375-4381.
16. Laemmli, U. K. 1970. Cleavage of structure proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
17. Larsson, A. M., R. Andersson, J. Stahlberg, L. Kenne, and T. A. Jones. 2003. Dextranase from Penicillium minioluteum: Reaction course, crystal structure, and product complex. Structure 11: 1111-1121.
18. Lee, J. H., S. O. Lee, G. O. Lee, E. S. Seo, S. S. Chang, S. K. Yoo, D. W. Kim, D. F. Day, and D. Kim. 2003. Transglycosylation reaction and raw starch hydrolysis by novel carbohydrolase from Lipomyces starkeyi. Biotechnol. Bioprocess Eng. 8: 106-111.
19. Macauley-Patrick, S., M. L. Fazenda, B. Mcneil, and L. M. Harvey. 2005. Heterologous protein production using the Pichia pastoris expression system. Yeast. 22: 249-270.
20. Rachel, D. and T. W. H. Milton. 2005. Expression of heterologous proteins in Pichia pastoris: A useful experimental tool in protein engineering and production. J. Mol. Recognit. 18: 119-138.
21. Roca, H., B. Garcia, E. Rodriguez, D. Mateu, L. Coroas, J. Cremata, R. Garcia, T. Pons, and J. Delgado. 1996. Cloning of Penicillium minioluteum gene encoding dextranase and its expression in Pichia pastoris. Yeast 12: 1187-1200.
22. Rose, M. D., F. Winstern, and P. Hieter. 1990. Methods in Yeast Genetics: A Laboratory Course. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
23. Ryu, S. J., D. Kim, H. J. Ryu, S. Chiba, A. Kimura, and D. F. Day. 2000. Purification and partial characterization of a novel glucanhydrolase from Lipomyces starkeyi KSM22 and its use for inhibition of insoluble glucan formation. Biosci. Biotechnol. Biochem. 64: 223-228.
24. R of high copy number transformants of Pichia pastoris for high-level foreign gene expression. BioTechnology 12: 181-184.
25. Yang, P. L., P. J. Shi, Y. R. Wang, Y. G. Bai, K. Meng, H. Y. Luo, T. H. Yuan, and B. Yao. 2007. Cloning and overexpression of a Paenibacillus β-glucanase in Pichia pastoris: Purification and characterization of the recombinant enzyme. J. Microbiol. Biotechnol. 17: 58-66.
26. Xu, B., Y. J. Yang, and Z. X. Huang. 2006. Cloning and overexpression of gene encoding the pullulanase from Bacillus maganoensis in Pichia pastoris. J. Microbiol. Biotechnol. 16: 1185-1191.
27. Zinchenko, O. N., A. G. Lobanok, Z. A. Rozhkaza, and V. I. Shishlo. 1989. Biosynthesis and properties of a dextranase from Lipomyces kononenkoae. Microbiology. 58: 453-457.