Culture-independent methods for studying environmental microorganisms: Methods, application, and perspective

Applied Microbiology and Biotechnology

Volumn 93, Issue 3, 2012, Pages 993-1003

  Su, Can ^a   Lei, Liping ^b   Duan, Yanqing ^c   Zhang, Ke Qin ^a   Yang, Jinkui ^a  

^a YUNNAN UNIVERSITY   (China)

^b Tobacco Agricultural Science Institute of Yunnan Province   (China)

^c Technology Centre of Hongyun Honghe Tobacco (Group) Co Ltd   (China)

Author keywords

Culture independent methods; Microbial community; Molecular analysis; Small subunit ribosomal RNA gene

Indexed keywords

CULTURE-INDEPENDENT METHODS; DIRECT AMPLIFICATION; DISTRIBUTION PATTERNS; ENVIRONMENTAL MICROBIOLOGY; FLUORESCENCE IN SITU HYBRIDIZATION; GENOMICS; GRADIENT GEL ELECTROPHORESIS; IN-SITU; METAGENOMICS; MICROBIAL COMMUNITIES; MICROBIAL DIVERSITY; MICROBIAL ECOLOGY; MOLECULAR ANALYSIS; MOLECULAR METHODS; MOLECULAR TECHNIQUES; NANO SCALE; QUANTITATIVE POLYMERASE CHAIN REACTION; RAMAN MICROSPECTROSCOPY; RESTRICTION FRAGMENT LENGTH POLYMORPHISMS; RIBOSOMAL RNA; SEQUENCING METHOD; SMALL SUBUNITS; TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISMS;

CELL CULTURE; ECOLOGY; ELECTROPHORESIS; FLUORESCENCE MICROSCOPY; GENES; MICROORGANISMS; RNA; SECONDARY ION MASS SPECTROMETRY;

POLYMERASE CHAIN REACTION;

WATER;

ELECTROKINESIS; GENOMICS; MICROBIAL COMMUNITY; MICROBIAL ECOLOGY; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION; POLYMORPHISM; SPECIES DIVERSITY;

AIR; ASCOMYCETES; BACTEROIDETES; BETAPROTEOBACTERIA; CAVE; CULTURE INDEPENDENT METHOD; CULTURE TECHNIQUE; DELTAPROTEOBACTERIA; DENATURING GRADIENT GEL ELECTROPHORESIS; DNA EXTRACTION; FLUORESCENCE IN SITU HYBRIDIZATION; FOOD ORGANISM; GENE AMPLIFICATION; GENOMICS; HUMAN; HYPOCREALES; INTERMETHOD COMPARISON; ISOLATION PROCEDURE; LACTOBACILLUS; METAGENOMICS; METAPROTEOMICS; METATRANSCRIPTOMICS; MICROARRAY ANALYSIS; MICROBIAL COMMUNITY; MICROBIAL DIVERSITY; MOLECULAR CLONING; MYCOPLASMA OVIPNEUMONIAE; NONHUMAN; PLANT; POLYMERASE CHAIN REACTION; PROTEOMICS; RAMAN MICROSPECTROMETRY; RAMAN SPECTROMETRY; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; REVIEW; SEQUENCE ANALYSIS; SINGLE CELL GENOMICS; SINGLE STRAND CONFORMATION POLYMORPHISM; SOIL; TRANSCRIPTOMICS;

ANIMALS; BACTERIA; ECOSYSTEM; ELECTROPHORESIS; ENVIRONMENTAL MICROBIOLOGY; GENETIC TECHNIQUES; HUMANS; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; RNA, RIBOSOMAL;

EID: 84856373733     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-011-3800-7     Document Type: Review

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