Simultaneous assessment of Asp isomerization and Asn deamidation in recombinant antibodies by LC-MS following incubation at elevated temperatures

PLoS ONE

Volumn 7, Issue 1, 2012, Pages

  Diepold, Katharina ^a   Bomans, Katrin ^a   Wiedmann, Michael ^a   Zimmermann, Boris ^a   Petzold, Andreas ^a   Schlothauer, Tilman ^a   Mueller, Robert ^b   Moritz, Bernd ^b   Stracke, Jan Olaf ^a   Mølhøj, Michael ^a   Reusch, Dietmar ^a   Bulau, Patrick ^a  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARAGINE; ASPARTIC ACID; BIOLOGICAL MARKER; CHLORIDE; HISTIDINE; IMMUNOGLOBULIN G1 ANTIBODY; RECOMBINANT ANTIBODY; AMIDE; ANTIBODY; IMMUNOGLOBULIN G; RECOMBINANT PROTEIN;

ALKYLATION; ANTIBODY DETECTION; ARTICLE; BINDING SITE; CHEMICAL REACTION; CONTROLLED STUDY; DEAMINATION; FERMENTATION; HIGH TEMPERATURE PROCEDURES; INCUBATION TEMPERATURE; ISOMERIZATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDE MAPPING; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN DOMAIN; PROTEIN PURIFICATION; REDUCTION; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; CHO CELL; COMPLEMENTARITY DETERMINING REGION; CRICETULUS; GENETICS; HAMSTER; ISOMERISM; METABOLISM; METHODOLOGY; PH; SEQUENCE HOMOLOGY; SURFACE PLASMON RESONANCE; TEMPERATURE;

AMIDES; AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES; ASPARAGINE; ASPARTIC ACID; CHO CELLS; CHROMATOGRAPHY, LIQUID; COMPLEMENTARITY DETERMINING REGIONS; CRICETINAE; CRICETULUS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; ISOMERISM; MASS SPECTROMETRY; PROTEIN BINDING; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SURFACE PLASMON RESONANCE; TEMPERATURE;

EID: 84855860373     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0030295     Document Type: Article

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