Isomerization of an aspartic acid residue in the complementarity-determining regions of a recombinant antibody to human IgE: Identification and effect on binding affinity

Biochemistry

Volumn 35, Issue 6, 1996, Pages 1897-1903

  Cacia, Jerry ^a   Keck, Rodney ^a   Presta, Leonard G ^a   Frenz, John ^a  

^a GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARTIC ACID; IMMUNOGLOBULIN E; IMMUNOGLOBULIN E ANTIBODY; MONOCLONAL ANTIBODY; OMALIZUMAB; RECOMBINANT MONOCLONAL ANTIBODY E 25; UNCLASSIFIED DRUG;

ANTIBODY COMBINING SITE; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; CHROMATOGRAPHY; CRYSTAL STRUCTURE; ISOMERISM; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANTIBODIES, ANTI-IDIOTYPIC; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; ASPARTIC ACID; HUMANS; IMMUNOGLOBULIN E; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MOLECULAR STRUCTURE; POINT MUTATION; RECOMBINANT PROTEINS; STEREOISOMERISM; VARIATION (GENETICS);

EID: 0030022071     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi951526c     Document Type: Article

References (24)

1. Abe, N., & Inouye, K. (1993) J. Biochem. Biophys. Methods 27, 215-227.
2. Allen, G. (1981) in Sequencing of Proteins and Peptides (Work, T. S., & Burdon, R. H., Eds.) pp 183-185, Elsevier, North Holland.
3. Aswad, D. W. (1984) J. Biol. Chem. 259, 10714-10721.
4. Bernstein, F. C., Koetzle, T. F., Williams, G. J. B., Meyer, E. F., Jr., Brice, M. D., Rodgers, J. R., Kennard, O., Shimanouchi, T., & Tasumi, T. (1977) J. Mol. Biol. 112, 535-542.
5. Bhat, T. N., Bentley, G. A., Boulot, G., Green, M. I., Tello, D., Dall'Acqua, W., Souchon, H., Schwarz, F. P., Mariuzza, R. A., & Poljak, R. J. (1994) Proc. Natl. Acad. Sci. U.S.A 91, 1089-1093.
6. Brunger, A. T., Leahy, D. J., Hynes, T. R., & Fox, R. O. (1991) J. Mol. Biol. 221, 239-256.
7. Chang, T. W., Davis, F. M., Sun, N. C., Sun, C. R. Y., MacGlashan, D. W., Jr., & Hamilton, R. G. (1990) Bio/Technology 8, 122-126.
8. Geiger, T., & Clarke, S. (1987) J. Biol. Chem. 262, 785-794.
9. Ghiara, J. B., Stura, E. A., Stanfield, R. L., Profy, A. T., & Wilson, I. A. (1994) Science 264, 82-85.
10. Grebenau, R. C., Goldenberg, D. M., Chang, C. H., Koch, G. A., Gold, D. V., Kunz, A., & Hansen, H. A. (1992) Mol. Immunol. 29, 751-758.
11. Harris, R. J., Murnane, A. A., Utter, S. L., Wagner, K. L., Cox, E. T., Polastri, G. D., Helder, J. C., & Sliwkowski, M. B. (1993) Bio/Technology 11, 1293-1297.
12. Jones, P. T., Dear, P. H., Foote, J., Neuberger, M. S., & Winter, G. (1986) Nature (London) 321, 522-525.
13. Jungbauer, A., Tauer, C., Wenisch, E., Karola, U., Brunner, J., Purtscher, M., Steindl, F., & Buchacher, A. (1990) J. Chromatogr. 512, 157-163.
14. Kabat, E. A., Wu, T. T., Perry, H. M., Gottesman, K. S., & Foeller, C. (1991) in Sequences of Proteins of Immunological Interest, Fifth Ed., U.S. Department of Health and Human Services, NIH Publication 91-3242.
15. Kwong, M. Y., & Harris, R. J. (1994) Protein Sci. 3, 147-149.
16. McDonough, J. P., Jue, R. A., Furman, T. C., Bartholomew, R. W., Vlahos, C. J., & Hochschwender, S. M. (1989) J. Cell Biochem. 13A (suppl.), 99.
17. Morimoto, K., & Inouye, K. (1991) J. Biochem. Biophys. Methods 24, 107-117.
18. Neblock, D. S., Chang, C. S., Mascelli, M. A., Fleek, M., Stumpo, L., Cullen, M. M., & Daddona, P. E. (1992) Biaconjugate Chem. 3, 126-131.
19. Oliyai, C., & Borchardt, R. T. (1993) Pharm. Res. 10, 95-102.
20. Ota, I. M., & Clarke, S. (1989) J. Biol. Chem. 264, 54-60.
21. Padlan, E. A., Silverton, E. W., Sheriff, S., Cohen, G. H., Smith-Gill, S. J., & Davies, D. R. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 5938-5942.
22. Presta, L. G., Lahr, S. J., Shields, R. L., Porter, J. P., Gorman, C. M., Fendly, B. M., & Jardieu, P. M. (1993) J. Immunol. 151, 2623-2632.
23. Rini, J. M., Stanfield, R. L., Stura, E. A., Salinas, P. A., Profy, A. T., & Wilson, I. A. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 6325-6329.
24. Saul, F. A., & Poljak, R. J. (1992) Proteins 14, 363-371.