Accumulation of succinimide in a recombinant monoclonal antibody in mildly acidic buffers under elevated temperatures

Pharmaceutical Research

Volumn 24, Issue 6, 2007, Pages 1145-1156

  Chu, Grace C ^a   Chelius, Dirk ^a   Xiao, Gang ^a   Khor, Hui K ^a   Coulibaly, Sururat ^a   Bondarenko, Pavel V ^a  

^a AMGEN INC   (United States)

Author keywords

Antibody; Hydrolysis; Ion exchange; Mass spectrometry; Succinimide

Indexed keywords

ASPARTIC ACID; BUFFER; IGG2 ANTIBODY; MONOCLONAL ANTIBODY; RECOMBINANT ANTIBODY; SUCCINIMIDE; UNCLASSIFIED DRUG;

ACIDITY; ALKALINITY; ARTICLE; CATION EXCHANGE; HIGH TEMPERATURE; HYDROLYSIS; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN MODIFICATION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; TEMPERATURE DEPENDENCE; THERMODYNAMICS;

ACIDS; AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; BUFFERS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; HEAT; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SUCCINIMIDES;

EID: 34249059679     PISSN: 07248741     EISSN: 1573904X     Source Type: Journal    
DOI: 10.1007/s11095-007-9241-4     Document Type: Article

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