Identification of potential sites for tryptophan oxidation in recombinant antibodies using tert-butylhydroperoxide and quantitative LC-MS

PLoS ONE

Volumn 6, Issue 3, 2011, Pages

  Hensel, Miriam ^a   Steurer, Rebecca ^a   Fichtl, Juergen ^a   Elger, Carsten ^a   Wedekind, Frank ^a   Petzold, Andreas ^a   Schlothauer, Tilman ^a   Molhoj, Michael ^a   Reusch, Dietmar ^a   Bulau, Patrick ^a  

^a ROCHE DIAGNOSTICS GMBH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR 2; IMMUNOGLOBULIN G1 ANTIBODY; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; METHIONINE; RECOMBINANT ANTIBODY; SOLVENT; TERT BUTYL HYDROPEROXIDE; TRYPTOPHAN; ANTIBODY; RECOMBINANT PROTEIN;

AMINO ACID ANALYSIS; AMINO ACID METABOLISM; ARTICLE; CHEMICAL BINDING; CONTROLLED STUDY; HEAVY CHAIN; HIGH TEMPERATURE PROCEDURES; LIGHT CHAIN; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDE MAPPING; PROTEIN STABILITY; QUANTITATIVE ANALYSIS; STRUCTURE ANALYSIS; AMINO ACID SEQUENCE; CHEMISTRY; DRUG EFFECT; METABOLISM; MOLECULAR GENETICS; OXIDATION REDUCTION REACTION; STANDARD; SURFACE PLASMON RESONANCE; ULTRAVIOLET RADIATION;

AMINO ACID SEQUENCE; ANTIBODIES; CHROMATOGRAPHY, LIQUID; MASS SPECTROMETRY; METHIONINE; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; REFERENCE STANDARDS; SURFACE PLASMON RESONANCE; TERT-BUTYLHYDROPEROXIDE; TRYPTOPHAN; ULTRAVIOLET RAYS;

EID: 79952291899     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0017708     Document Type: Article

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