Simvastatin promotes osteogenic differentiation of mouse embryonic stem cells via canonical Wnt/β-catenin signaling

Molecules and Cells

Volumn 32, Issue 5, 2011, Pages 437-444

  Qiao, Ling Juan ^a   Kang, Kyung Lhi ^a   Heo, Jung Sun ^a  

^a Kyung Hee University   (South Korea)

Author keywords

Canonical Wnt catenin signaling; Embryonic stem cells; Osteogenic differentiation; Simvastatin

Indexed keywords

ALKALINE PHOSPHATASE; BETA CATENIN; COLLAGEN TYPE 1; DICKKOPF 1 PROTEIN; MESSENGER RNA; OSTEOCALCIN; OSTEOPONTIN; SIMVASTATIN; SMALL INTERFERING RNA; TRANSCRIPTION FACTOR OSTERIX; TRANSCRIPTION FACTOR RUNX2; WNT PROTEIN;

ANIMAL CELL; ARTICLE; BONE MINERALIZATION; CELL DIFFERENTIATION; CONCENTRATION RESPONSE; DRUG EFFECT; EMBRYONIC STEM CELL; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC TRANSFECTION; MOUSE; NONHUMAN; OSTEOBLAST; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; UPREGULATION; WNT SIGNALING PATHWAY;

ANIMALS; BETA CATENIN; CELL DIFFERENTIATION; CELL LINE; DOWN-REGULATION; EMBRYONIC STEM CELLS; HYDROXYMETHYLGLUTARYL-COA REDUCTASE INHIBITORS; IMMUNOHISTOCHEMISTRY; MICE; OSTEOGENESIS; RNA, SMALL INTERFERING; SIGNAL TRANSDUCTION; SIMVASTATIN; TRANSFECTION; WNT PROTEINS;

EID: 84855677996     PISSN: 10168478     EISSN: 02191032     Source Type: Journal    
DOI: 10.1007/s10059-011-0107-6     Document Type: Article

References (34)

1. Baek, K.H., Lee, W.Y., Oh, K.W., Tae, H.J., Lee, J.M., Lee, E.J., Han, J.H., Kang, M.I., Cha, B.Y., Lee, K.W., et al. (2005). The effect of simvastatin on the proliferation and differentiation of human bone marrow stromal cells. J. Korean Med. Sci. 20, 438-444.
2. Bielby, R.C., Boccaccini, A.R., Polak, J.M., and Buttery, L.D. (2004). In vitro differentiation and in vivo mineralization of osteogenic cells derived from human embryonic stem cells. Tissue Eng. 10, 1518-1525. (Pubitemid 39557855)
3. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
4. Bratt-Leal, A.M., Carpenedo, R.L., and McDevitt, T.C. (2009). Engineering the embryoid body microenvironment to direct embryonic stem cell differentiation. Biotechnol. Prog. 25, 43-51.
5. Buttery, L.D., Bourne, S., Xynos, J.D., Wood, H., Hughes, F.J., Hughes, S.P., Episkopou, V., and Polak, J.M. (2001). Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells. Tissue Eng. 7, 89-99. (Pubitemid 32175965)
6. Canalis, E., Economides, A.N., and Gazzerro, E. (2003) Bone morphogenetic proteins, their antagonists, and the skeleton. Endocr. Rev. 24, 218-235. (Pubitemid 36515285)
7. Chadwick, K., Wang, L., Li, L., Menendez, P., Murdoch, B., Rouleau, A., and Bhatia, M. (2003). Cytokines and BMP-4 promote hematopoietic differentiation of human embryonic stem cells. Blood 102, 906-915. (Pubitemid 36917782)
8. Chaudhry, G.R., Yao, D., Smith, A., and Hussain, A. (2004). Osteogenic Cells Derived From Embryonic Stem Cells Produced Bone Nodules in Three-Dimensional Scaffolds. J. Biomed. Biotechnol. 2004, 203-210. (Pubitemid 40128956)
9. Chen, P.Y., Sun, J.S., Tsuang, Y.H., Chen, M.H., Weng, P.W., and Lin, F.H. (2010). Simvastatin promotes osteoblast viability and differentiation via Ras/Smad/Erk/BMP-2 signaling pathway. Nutr. Res. 30, 191-199.
10. Grebenová, D., Kuzelová, K., Smetana, K., Pluskalová, M., Cajthamlová, H., Marinov, I., Fuchs, O., Soucek, J., Jarolím, P., and Hrkal, Z. (2003). Mitochondrial and endoplasmic reticulum stress-induced apoptotic pathways are activated by 5-aminolevulinic acid-based photodynamic therapy in HL60 leukemia cells. J. Photochem. Photobiol. B. 69, 71-85. (Pubitemid 36336463)
11. Guo, A.J., Choi, R.C., Cheung, A.W., Chen, V.P., Xu, S.L., Dong, T.T., Chen, J.J., and Tsim, K.W. (2011). Baicalin, a Flavone, Induces the Differentiation of Cultured Osteoblasts: An action via the Wnt/beta-catenin signaling pathway. J. Biol. Chem. 286, 27882-27893.
12. Harada, H., Tagashira, S., Fujiwara, M., Ogawa, S., Katsumata, T., Yamaguchi, A., Komori, T., and Nakatsuka, M. (1999). Cbfa1 isoforms exert functional differences in osteoblast differentiation. J. Biol. Chem. 274, 6972-6978.
13. Heng, B.C., Cao, T., Stanton, L.W., Robson, P., and Olsen, B. (2004). Strategies for directing the differentiation of stem cells into the osteogenic lineage in vitro. J. Bone Miner. Res. 19, 1379-1394. (Pubitemid 41094339)
14. Heo, J.S., and Lee, J.C. (2011). β-Catenin mediates cyclic strainstimulated cardiomyogenesis in mouse embryonic stem cells through ROS-dependent and integrin-mediated PI3K/Akt pathways. J. Cell. Biochem. 112, 1880-1889.
15. Kato, M., Patel, M.S., Levasseur, R., Lobov, I., Chang, B.H., Glass 2nd, D.A., Hartmann, C., Li, L., Hwang, T.H., Brayton, C.F., et al. (2002). Cbfa1-independent decrease in osteoblast proliferation, osteopenia, and persistent embryonic eye vascularization in mice deficient in Lrp5, a Wnt coreceptor. J. Cell Biol. 157, 303-314. (Pubitemid 34839826)
16. Kern, B., Shen, J., Starbuck, M., and Karsenty, G. (2001). Cbfa1 contributes to the osteoblast-specific expression of type I collagen genes. J. Biol. Chem. 276, 7101-7107.
17. Kim, S.Y., Kim, S., Yun-Choi, H.S., and Jho, E.H. (2011). Wnt5a potentiates U46619-induced platelet aggregation via the PI3K/ Akt pathway. Mol. Cells [Epub ahead of print].
18. Lin, C.L., Cheng, H., Tung, C.W., Huang, W.J., Chang, P.J., Yang, J.T., and Wang, J.Y. (2008). Simvastatin reverses high glucoseinduced apoptosis of mesangial cells via modulation of Wnt signaling pathway. Am. J. Nephrol. 28, 290-297.
19. Maeda, T., Matsunuma, A., Kawane, T., and Horiuchi, N. (2001). Simvastatin promotes osteoblast differentition and mineralization in MC3T3-E1 cells. Biochem. Biophys. Res. Commun. 280, 874-877. (Pubitemid 32924513)
20. Montagnani, A., Gonnelli, S., Cepollaro, C., Pacini, S., Campagna, M.S., Franci, M.B., Lucani, B., and Gennari, C. (2003). Effect of simvastatin treatment on bone mineral density and bone turnover in hypercholesterolemic postmenopausal women: A 1-year longitudinal study. Bone 32, 427-433. (Pubitemid 36410317)
21. Mundy, G., Garrett, R., Harris, S., Chan, J., Chen, D., Rossini, G., Boyce, B., Zhao, M., and Gutierrez, G. (1999). Stimulation of bone formation in vitro and in rodents by statins. Science 286, 1946-1949. (Pubitemid 129515901)
22. Nusse, R. (2005). Wnt signaling in disease and in development. Cell Res. 15, 28-32. (Pubitemid 41653961)
23. Pagkalos, J., Cha, J.M., Kang, Y., Heliotis, M., Tsiridis, E., and Mantalaris, A. (2010). Simvastatin induces osteogenic differenttiation of murine embryonic stem cells. J. Bone Miner. Res. 25, 2470-2478.
24. Phillips, B.W., Belmonte, N., Vernochet, C., Ailhaud, G., and Dani, C. (2001). Compactin enhances osteogenesis in murine embryonic stem cells. Biochem. Biophys. Res. Commun. 284, 478-484. (Pubitemid 32918002)
25. Rawadi, G., Vayssiere, B., Dunn, F., Baron, R., and Roman-Roman, S., (2003). BMP-2 controls alkaline phosphatase expression and osteoblast mineralization by a Wnt autocrine loop. J. Bone Miner. Res. 18, 1842-1853. (Pubitemid 37322562)
26. Ruiz-Gaspa, S., Nogues, X., Enjuanes, A., Monllau, J.C., Blanch, J., Carreras, R., Mellibovsky, L., Grinberg, D., Balcells, S., Díez-Perez, A., et al. (2007). Simvastatin and atorvastatin enhance gene expression of collagen type 1 and osteocalcin in primary human osteoblasts and MG-63 cultures. J. Cell. Biochem. 101, 1430-1438. (Pubitemid 47242774)
27. Sakoda, K., Yamamoto, M., Negishi, Y., Liao, J.K., Node, K., and Izumi, Y. (2006). Simvastatin decreases IL-6 and IL-8 production in epithelial cells. J. Dent. Res. 85, 520-523. (Pubitemid 43945489)
28. Song, C., Guo, Z., Ma, Q., Chen, Z., Liu, Z., Jia, H., and Dang, G. (2003). Simvastatin induces osteoblastic differentiation and inhibits adipocytic differentiation in mouse bone marrow stromal cells. Biochem. Biophys. Res. Commun. 308, 458-462. (Pubitemid 36945513)
29. Sottile, V., Thomson, A., and McWhir, J. (2003). In vitro osteogenic differentiation of human ES cells. Cloning Stem Cells 5, 149-155. (Pubitemid 36877165)
30. Wang, J.W., Xu, S.W., Yang, D.S., and Lv, R.K. (2007). Locally applied simvastatin promotes fracture healing in ovariectomized rat. Osteoporos. Int. 18, 1641-1650. (Pubitemid 47620312)
31. Xu, R.H., Chen, X., Li, D.S., Li, R., Addicks, G.C., Glennon, C., Zwaka, T.P., and Thomson, J.A. (2002). BMP4 initiates human embryonic stem cell differentiation to trophoblast. Nat. Biotechnol. 20, 1261-1264. (Pubitemid 35462493)
32. Zhang, Y., Wang, Y., Li, X., Zhang, J., Mao, J., Li, Z., Zheng, J., Li, L., Harris, S., and Wu, D. (2004). The LRP5 high-bone-mass G171V mutation disrupts LRP5 interaction with Mesd. Mol. Cell. Biol. 24, 4677-4684. (Pubitemid 38668035)
33. Zhang, L., Zhang, L., Tian, F., Han, D., Niu, J., and Liu, X. (2009). Effect of simvastatin on mRNA expressions of some components of Wnt signaling pathway in differentiation process of osteoblasts derived from BMSCs of rats. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 23, 1371-1375.
34. zur Nieden, N.I., Kempka, G., Rancourt, D.E., and Ahr, H.J. (2005). Induction of chondro-, osteo-and adipogenesis in embryonic stem cells by bone morphogenetic protein-2: Effect of cofactors on differentiating lineages. BMC Dev. Biol. 5, 1-15.