Inducible raptor and rictor knockout mouse embryonic fibroblasts

Methods in Molecular Biology

Volumn 821, Issue , 2012, Pages 267-278

  Cybulski, Nadine ^a   Zinzalla, Vittoria ^a   Hall, Michael N ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

Author keywords

iRapKO; iRicKO; Knockout; Mouse embryonic fibroblasts; mTOR; Raptor; Rictor; Signaling

Indexed keywords

CARRIER PROTEIN; MTORC1 COMPLEX, MOUSE; PROTEIN; RAPTOR PROTEIN, MOUSE; RICTOR PROTEIN, MOUSE; TORC2 PROTEIN, MOUSE; TRANSACTIVATOR PROTEIN;

ANIMAL; ARTICLE; CELL LINE; CYTOLOGY; DRUG ANTAGONISM; FEMALE; FETUS; FIBROBLAST; GENE INACTIVATION; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; METABOLISM; METHODOLOGY; MOUSE; MOUSE MUTANT; PREGNANCY; SIGNAL TRANSDUCTION;

ANIMALS; CARRIER PROTEINS; CELL LINE; FEMALE; FETUS; FIBROBLASTS; GENE KNOCKOUT TECHNIQUES; GENETIC VECTORS; MICE; MICE, KNOCKOUT; PREGNANCY; PROTEINS; SIGNAL TRANSDUCTION; TRANS-ACTIVATORS; TRANSFECTION;

MAMMALIA; RAPTORES;

EID: 84555187735     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-430-8_16     Document Type: Article

References (29)

1. Wullschleger S, Loewith R, Hall MN (2006) TOR signaling in growth and metabolism. Cell 124:471-484 (Pubitemid 43199434)
2. Loewith R, Jacinto E, Wullschleger S et al (2002) Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Mol Cell 10:457-468 (Pubitemid 35284167)
3. Kim D H, Sarbassov D D, Ali SM et al (2002) mTOR interacts with raptor to form a nutrientsensitive complex that signals to the cell growth machinery. Cell 110:163-175 (Pubitemid 34876545)
4. Hara K, Maruki Y, Long X et al (2002) Raptor, a binding partner of target of rapamycin (TOR), mediates TOR action. Cell 110: 177-189 (Pubitemid 34876546)
5. Soulard A, Hall M N (2007) SnapShot: mTOR signaling. Cell 129:434
6. Laplante M, Sabatini D M (2009) mTOR signaling at a glance. J Cell Sci 122:3589-3594
7. Choo A Y, Yoon S O, Kim S G et al (2008) Rapamycin differentially inhibits S6Ks and 4E-BP1 to mediate cell-type-specific repression of mRNA translation. Proc Natl Acad Sci USA 105:17414-17419
8. Feldman M E, Apsel B, Uotila A et al (2009) Active-site inhibitors of mTOR target rapamycin-resistant outputs of mTORC1 and mTORC2. PLoS Biol 7:e38
9. Thoreen C C, Kang S A, Chang J W et al (2009) An ATP-competitive mammalian target of rapamycin inhibitor reveals rapamycin-resistant functions of mTORC1. J Biol Chem 284:8023-8032
10. Cybulski N, Hall M N (2009) TOR complex 2: a signaling pathway of its own. Trends Biochem Sci 34:620-627
11. Jacinto E, Facchinetti V, Liu D et al (2006) SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell 127:125-137 (Pubitemid 44466632)
12. Jacinto E, Loewith R, Schmidt A et al (2004) Mammalian TOR complex 2 controls the actin cytoskeleton and is rapamycin insensitive. Nat Cell Biol 6:1122-1128 (Pubitemid 39468014)
13. Sarbassov D D, Ali S M, Kim D H et al (2004) Rictor, a novel binding partner of mTOR, defines a rapamycin-insensitive and raptorindependent pathway that regulates the cytoskeleton. Curr Biol 14:1296-1302 (Pubitemid 38991819)
14. Frias M A, Thoreen C C, Jaffe J D et al (2006) mSin1 is necessary for Akt/PKB phosphorylation, and its isoforms define three distinct mTORC2s. Curr Biol 16:1865-1870 (Pubitemid 44354144)
15. Yang Q, Inoki K, Ikenoue T et al (2006) Identification of Sin1 as an essential TORC2 component required for complex formation and kinase activity. Genes Dev 20:2820-2832 (Pubitemid 44771744)
16. Garcia-Martinez J M, Alessi D R (2008) mTOR complex 2 (mTORC2) controls hydrophobic motif phosphorylation and activation of serumand glucocorticoid-induced protein kinase 1 (SGK1). Biochem J 416:375-385
17. Hresko R C, Mueckler M (2005) mTOR. RICTOR is the Ser473 kinase for Akt/protein kinase B in 3T3-L1 adipocytes. J Biol Chem 280:40406-40416 (Pubitemid 41780527)
18. Sarbassov D D, Guertin D A, Ali SM et al (2005) Phosphorylation and regulation of Akt/PKB by the rictor-mTOR complex. Science 307:1098-1101 (Pubitemid 40262113)
19. Gangloff Y G, Mueller M, Dann S G et al (2004) Disruption of the mouse mTOR gene leads to early postimplantation lethality and prohibits embryonic stem cell development. Mol Cell Biol 24:9508-9516 (Pubitemid 39391687)
20. Guertin D A, Stevens D M, Thoreen C C et al (2006) Ablation in mice of the mTORC components raptor, rictor, or mLST8 reveals that mTORC2 is required for signaling to Akt-FOXO and PKCalpha, but not S6K1. Dev Cell 11:859-871 (Pubitemid 44804279)
21. Shiota C, Woo J T, Lindner J et al (2006) Multiallelic disruption of the rictor gene in mice reveals that mTOR complex 2 is essential for fetal growth and viability. Dev Cell 11:583-589 (Pubitemid 44430900)
22. Nagy A (2000) Cre recombinase: the universal reagent for genome tailoring. Genesis 26:99-109 (Pubitemid 30158089)
23. Bentzinger C F, Romanino K, Cloetta D et al (2008) Skeletal muscle-specific ablation of raptor, but not of rictor, causes metabolic changes and results in muscle dystrophy. Cell Metab 8:411-424
24. Cybulski N, Polak P, Auwerx J et al (2009) mTOR complex 2 in adipose tissue negatively controls whole-body growth. Proc Natl Acad Sci USA 106:9902-9907
25. Polak P, Cybulski N, Feige J N et al (2008) Adipose-specific knockout of raptor results in lean mice with enhanced mitochondrial respiration. Cell Metab 8:399-410
26. Feil R, Brocard J, Mascrez B et al (1996) Ligandactivated site-specific recombination in mice. Proc Natl Acad Sci USA 93:10887-10890
27. Leone D P, Genoud S, Atanasoski S et al (2003) Tamoxifen-inducible glia-specific Cre mice for somatic mutagenesis in oligodendrocytes and Schwann cells. Mol Cell Neurosci 22:430-440 (Pubitemid 36513567)
28. Patursky-Polischuk I, Stolovich-Rain M, Hausner-Hanochi M et al (2009) The TSCmTOR pathway mediates translational activation of TOP mRNAs by insulin largely in a raptor- or rictor-independent manner. Mol Cell Biol 29:640-649
29. Facchinetti V, Ouyang W, Wei H et al (2008) The mammalian target of rapamycin complex 2 controls folding and stability of Akt and protein kinase C. EMBO J 27:1932-1943