The construction and characterization of a bifunctional EGFP/sAPRIL fusion protein

Applied Microbiology and Biotechnology

Volumn 73, Issue 5, 2007, Pages 1114-1122

  Guan, Zhengbing ^a   Yao, Wenjuan ^a   Ye, Jilin ^a   Dan, Wenbing ^a   Shen, Jiayin ^a   Zhang, Shuangquan ^a  

^a NANJING NORMAL UNIVERSITY   (China)

Author keywords

APRIL; EGFP; Fusion protein; In vitro expression; LSCM

Indexed keywords

AFFINITY CHROMATOGRAPHY; ANTIBODIES; ANTIGENS; BIOASSAY; ENZYMES; ESCHERICHIA COLI; FLUORESCENCE;

APRIL; ENHANCED GREEN FLUORESCENT PROTEIN (EGFP); FUSION PROTEIN; IN VITRO EXPRESSION; LSCM;

PROTEINS;

ANTIGEN; APRIL PROTEIN; BOUND RECEPTOR C CELL MATURATION ANTIGEN; ENHANCED GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; METAL CHELATE; MONOCLONAL ANTIBODY;

ANTIBODY; BIOENGINEERING; COLIFORM BACTERIUM; FLUORESCENCE; IMMUNOASSAY; PROTEIN;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ANTIGEN DETECTION; ARTICLE; CELL PROLIFERATION; CELL STRAIN 3T3; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FLUORESCENCE ACTIVATED CELL SORTING; FLUORESCENCE SPECTROSCOPY; HUMAN; HUMAN CELL; IN VITRO STUDY; LEUKEMIA CELL LINE; MOUSE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS;

ANIMALS; B-CELL MATURATION ANTIGEN; CELLS, CULTURED; CHROMATOGRAPHY, AFFINITY; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; HEPARAN SULFATE PROTEOGLYCANS; HUMANS; JURKAT CELLS; LEUKOCYTES, MONONUCLEAR; LYMPHOCYTE ACTIVATION; MICE; MICROSCOPY, CONFOCAL; NIH 3T3 CELLS; PROTEIN BINDING; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, FLUORESCENCE; TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 13;

ESCHERICHIA COLI;

EID: 33845771327     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-006-0591-3     Document Type: Article

References (24)

1. Batten M, Groom J, Cachero TG, Qian F, Schneider P, Tschopp J, Browning JL, Mackay F (2000) BAFF mediates survival of peripheral immature B lymphocytes. J Exp Med 192:1453-1466
2. Cao P, Mei JJ, Diao ZY, Zhang SQ (2005) Expression, refolding and characterization of human soluble BAFF synthesized in Escherichia coli. Protein Expr Purif 41:199-206
3. Castigli E, Scott S, Dedeoglu F, Bryce P, Jabara H, Bhan AK, Mizoguchi E, Geha RS (2004) Impaired IgA class switching in APRIL-deficient mice. Proc Natl Acad Sci USA 101:3903-3908
4. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC (1994) Green fluorescent protein as a marker for gene expression. Science 263:802-805
5. Dillon SR, Gross JA, Ansell SM, Novak AJ (2006) An APRIL to remember: novel TNF ligands as therapeutic targets. Nat Rev Drug Discov 5:235-246
6. Guan ZB, Cao P, Ye JL, Zhang SQ (2006a) Cloning, soluble expression and characterization of human sBCMA. Sheng Wu Gong Cheng Xue Bao 22:46-51, Chinese
7. Guan ZB, Ye JL, Dan WB, Yao WJ, Zhang SQ (2006b) Cloning, expression and bioactivity of duck BAFF. Mol Immunol (in press).
8. Hahne M, Kataoka T, Schroter M, Hofmann K, Irmler M, Bodmer JL, Schneider P, Bornand T, Holler N, French LE, Sordat B, Rimoldi D, Tschopp J (1998) APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med 188:1185-1190
9. Hendriks J, Planelles L, Jong-Odding JD, Hardenberg G, Pals ST, Hahne M, Spaargaren M, Medema JP (2005) Heparan sulfate proteoglycan binding promotes APRIL-induced tumor cell proliferation. Cell Death Differ 12:637-648
10. Ingold K, Zumsteg A, Tardivel A, Huard B, Steiner Q, Cachero TG, Qiang F, Gorelik L, Kalled SL, Acha-Orbea H, Rennert PD, Tschopp J, Schneider P (2005) Identification of proteoglycans as the APRIL-specific binding partners. J Exp Med 201:1375-1383
11. Litinskiy MB, Nardelli B, Hilbert DM, He B, Schaffer A, Casali P, Cerutti A (2002) DCs induce CD40-independent immunoglobulin class switching through BLyS and APRIL. Nat Immunol 3:822-829
12. Lopez-Fraga M, Fernandez R, Albar JP, Hahne M (2001) Biologically active APRIL is secreted following intracellular processing in the Golgi apparatus by furin convertase. EMBO Rep 2:945-951
13. Mackay F, Schneider P, Rennert P, Browning J (2003) BAFF AND APRIL: a tutorial on B cell survival. Annu Rev Immunol 21:231-264
14. Marsters SA, Yan M, Pitti RM, Haas PE, Dixit VM, Ashkenazi A (2000) Interaction of the TNF homologues BLyS and APRIL with the TNF receptor homologues BCMA and TACI. Curr Biol 10:785-788
15. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55-63
16. Planelles L, Carvalho-Pinto CE, Hardenberg G, Smaniotto S, Savino W, Gomez-Caro R, Alvarez-Mon M, de Jong J, Eldering E, Martinez AC, Medema JP, Hahne M (2004) APRIL promotes B-1 cell-associated neoplasm. Cancer Cell 6:399-408
17. Rennert P, Schneider P, Cachero TG, Thompson J, Trabach L, Hertig S, Holler N, Qian F, Mullen C, Strauch K, Browning JL, Ambrose C, Tschopp J (2000) A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth. J Exp Med 192:1677-1684
18. Schneider P, MacKay F, Steiner V, Hofmann K, Bodmer JL, Holler N, Ambrose C, Lawton P, Bixler S, Acha-Orbea H, Valmori D, Romero P, Werner-Favre C, Zubler RH, Browning JL, Tschopp J (1999) BAFF, a novel ligand of tumor necrosis factor family, stimulates B cell growth. J Exp Med 189:1747-1756
19. Stauber RH, Horie K, Carney P, Hudson EA, Tarasova NI, Gaitanaris GA, Pavlakis GN (1998) Development and applications of enhanced green fluorescent protein mutants. Biotechniques 24:462-471
20. Stein JV, Lopez-Fraga M, Elustondo FA, Carvalho-Pinto CE, Rodriguez D, Gomez-Caro R, De Jong J, Martinez AC, Medema JP, Hahne M (2002) APRIL modulates B and T cell immunity. J Clin Invest 109:1587-1598
21. Tsien R (1998) The green fluorescent protein. Annu Rev Biochem 67:509-544
22. Wallweber JA, Compaan DM, Starovasnik MA, Hymowitz SG (2004) The crystal structure of a proliferation-inducing ligand, APRIL. J Mol Biol 343:283-290
23. Wu Y, Bressette D, Carrell JA, Kaufman T, Feng P, Taylor K, Gan Y, Cho YH, Garcia AD, Gollatz E, Dimke D, LaFleur D, Migone TS, Nardelli B, Wei P, Ruben SM, Ullrich SJ, Olsen HS, Kanakaraj P, Moore PA, Baker KP (2000) Tumor necrosis factor (TNF) receptor superfamily member TACI is a high affinity receptor for TNF family members APRIL and BLyS. J Biol Chem 275:35478-35485
24. Yu G, Boone T, Delaney J, Hawkins N, Kelley M, Ramakrishnan M, McCabe S, Qiu WR, Kornuc M, Xia XZ, Guo J, Stolina M, Boyle WJ, Sarosi I, Hsu H, Senaldi G, Theill LE (2000) APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat Immunol 1:252-256