The synthesis of sialylated oligosaccharides using a CMP-Neu5Ac synthetase/sialyltransferase fusion

Nature Biotechnology

Volumn 16, Issue 8, 1998, Pages 769-772

  Gilbert, Michel ^a   Bayer, Robert ^b   Cunningham, Anna Maria ^a   DeFrees, Shawn ^b   Gao, Yinghong ^b   Watson, David C ^a   Young, N Martin ^a   Wakarchuk, Warren W ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b CYTEL CORPORATION   (United States)

Author keywords

Carbohydrates; Glycobiology; Protein engineering

Indexed keywords

LACTOSE; OLIGOSACCHARIDE; PHOSPHOENOLPYRUVATE; SIALIC ACID; SIALYLTRANSFERASE; SYNTHETASE;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME SYNTHESIS; ESCHERICHIA COLI; NEISSERIA MENINGITIDIS; PRIORITY JOURNAL; PROTEIN ANALYSIS;

CATALYSIS; CHROMATOGRAPHY, AFFINITY; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; ION EXCHANGE; LACTOSE; MULTIENZYME COMPLEXES; N-ACETYLNEURAMINIC ACID; N-ACYLNEURAMINATE CYTIDYLYLTRANSFERASE; NEISSERIA MENINGITIDIS; NEURAMINIC ACIDS; OLIGOSACCHARIDES; PHOSPHOENOLPYRUVATE; PRECIPITATION; RECOMBINANT FUSION PROTEINS; SIALYLTRANSFERASES; SOLUBILITY; ULTRAFILTRATION;

ESCHERICHIA COLI; NEGIBACTERIA; NEISSERIA MENINGITIDIS;

EID: 0031879563     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0898-769     Document Type: Article

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