Feruloyl esterase activity of the Clostridium thermocellum cellulosome can be attributed to previously unknown domains of XynY and XynZ

Journal of Bacteriology

Volumn 182, Issue 5, 2000, Pages 1346-1351

  Blum, David L ^a,b   Kataeva, Irina A ^a   Li, Xin Liang ^a   Ljungdahl, Lars G ^a  

^a University of Georgia   (United States)

^b UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOHYDRATE DERIVATIVE; ESTERASE; FERULIC ACID; FERULOYL ESTERASE; ISOENZYME; RECOMBINANT ENZYME; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYLOSE;

ARTICLE; BACTERIAL MEMBRANE; CELL VACUOLE; CLOSTRIDIUM THERMOCELLUM; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; ORPINOMYCES; PRIORITY JOURNAL; PROTEIN DOMAIN; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; CARBOXYLIC ESTER HYDROLASES; CLOSTRIDIUM; DNA, BACTERIAL; ESCHERICHIA COLI; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS; SEQUENCE DELETION; SEQUENCE HOMOLOGY, AMINO ACID; XYLAN ENDO-1,3-BETA-XYLOSIDASE; XYLOSIDASES;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM THERMOCELLUM; CYNODON DACTYLON; ESCHERICHIA COLI; FUNGI; ORPINOMYCES; POSIBACTERIA; PROKARYOTA;

EID: 0033983751     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.5.1346-1351.2000     Document Type: Article

References (44)

1. Arakaki, C., R. Breves, A. Czihal, H. Baeumlein, B. Carillo, and J. Hofemeister. 1997. EMBL data library accession no. S58235.
2. Bayer, E. A., L. J. W. Shimon, Y. Shoham, and R. Lamed. 1998. Cellulosomes - structure and ultrastructure. J. Struct. Biol. 124:221-234.
3. Beauvais, A., M. Monod, J. P. Debeaupui, M. Diaquin, H. Kobayashi, and J. P. Latge. 1997. Biochemical and antigenic characterization of a new dipeptidyl-peptidase isolated from Aspergillus fumigatus. J. Biol. Chem. 272: 6238-6244.
4. Béguin, P., and M. Lemaire. 1996. The cellulosome: an exocellular, multiprotein complex specialized in cellulose degradation. Crit. Rev. Biochem. Mol. Biol. 31:201-236.
5. Blum, D. L., I. Kataeva, X.-L. Li, and L. G. Ljungdahl. 1998. Phenolic acid esterase activity of Clostridium thermocellum cellulosome is attributed to previously unknown domains of XynY and XynZ, p. 478. In K. Ohmiya, K. Hayashi, K. Sakka, Y. Kobayashi, S. Karita, and T. Kimura (ed.), Genetics, Biochemistry, and Ecology of Cellulose Degradation. Proceedings of Mie Bioforum 98. UNI Publishers Co., Tokyo, Japan.
6. Blum, D. L., X.-L. Li, H. Chen, and L. G. Ljungdahl. 1999. Characterization of an acetyl xylan esterase from the anaerobic fungus Orpinomyces sp. strain PC-2. Appl. Environ. Microbiol. 65:3990-3995.
7. Borneman, W. S., R. D. Hartley, D. S. Himmelsbach, and L. G. Ljungdahl. 1990. Assay for trans-p-coumaroyl esterase using a specific substrate from plant cell walls. Anal. Biochem. 190:129-133.
8. Borneman, W. S., L. G. Ljungdahl, R. D. Hartley, and D. E. Akin. 1993. Feruloyl and p-coumaroyl esterases from the anaerobic fungus Neocallimastix MC-2: properties and functions in plant cell wall degradation, p. 85-102. In M. P. Coughlan and G. Hazlewood (ed.), Hemicellulose and hemicellulases. Portland Press, Cambridge, United Kingdom.
9. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
10. Burland, V. D., G. Plunkett III, D. L. Daniels, and F. R. Blattner. 1993. DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication. Genomics 16:551-561.
11. Choi, S. K., and L. G. Ljungdahl. 1996. Dissociation of the cellulosome of Clostridium thermocellum in the presence of ethylenediaminetetraacetic acid occurs with the formation of truncated polypeptides. Biochemistry 35:4897-4905.
12. Coughlan, M. P., K. Hon-narai, H. Hon-nami, L. G. Ljungdahl, J. J. Paulin, and W. E. Rigsby. 1985. The cellulolytic enzyme complex of Clostridium thermocellum is very large. Biochem. Biophys. Res. Commun. 130:904-909.
13. Dalrymple, B. D., and Y. Swadling. 1997. Expression of a Butyrivibrio fibrisolvens E14 gene (cinB) encoding an enzyme with cinnamoyl esterase activity is negatively regulated by the product of an adjacent gene (cinR). Microbiology 143:1203-1210.
14. Dalrymple, B. D., Y. Swadling, D. H. Cybinski, and G.-P. Xue. 1996. Cloning of a gene encoding cinnamoyl ester hydrolase from the ruminal bacterium Butyrivibrio fibrisolvens E14 by a novel method. FEMS Microbiol. Lett. 143: 115-120.
15. de Vries, R. P., B. Michelsen, C. H. Poulsen, P. A. Kroon, R. H. H. van den Heuvel, C. B. Faulds, G. Williamson, J. P. T. W. van den Hombergh, and J. Visser. 1997. The faeA genes from Aspergillus niger and Aspergillus tubingensis encode ferulic acid esterases involved in degradation of complex cell wall polysaccharides. Appl. Environ. Microbiol. 63:4638-4644.
16. Dominguez, R., H. Souchon, S. Spinelli, Z. Dauter, K. S. Wilson, S. Chauvaux, P. Béguin, and P. M. Alzari. 1995. A common protein fold and similar active site in two distinct families of β-glycanases. Nat. Struct. Biol. 2:569-576.
17. Fanutti, C., T. Ponyi, G. W. Black, G. P. Hazlewood, and H. J. Gilbert. 1995. The conserved noncatalytic 40-residue sequence in cellulases and hemicellulases from anaerobic rumen fungi functions as a docking domain. J. Biol. Chem. 270:29314-29322.
18. Ferreira, L. M. A., T. M. Wood, G. Williamson, C. Faulds, G. P. Hazlewood, G. W. Black, and H. J. Gilbert, 1993. A modular esterase from Pseudomonas fluorescens subsp. cellulosa contains a non-catalytic cellulose-binding domain. Biochem. J. 294:349-355.
19. Flint, H. J., J. Martin, C. A. McPherson, A. S. Daniel, and J.-X. Zhang. 1993. A bifunctional enzyme, with separate xylanase and β-(1,3-1,4)-glucanase domains, encoded by the xynD gene of Ruminococcus flavefaciens. J. Bacteriol. 175:2943-2951.
20. Fontes, C. M. G. A., G. P. Hazlewood, E. Morag, J. Hall, B. H. Hirst, and H. J. Gilbert. 1995. Evidence for a general role for noncatalytic thermostabilizing domains in xylananses from thermophilic bacteria. Biochem. J. 307: 151-158.
21. Gilbert, H. J., G. P. Hazlewood, J. I. Laurie, C. G. Orpin, and G. P. Xue. 1992. Homologous catalytic domains in a rumen fungal xylanase: evidence for gene duplication and prokaryotic origin. Mol. Microbiol. 6:2065-2072.
22. Grépinet, O., M.-C. Chebrou, and P. Béguin. 1988. Purification of Clostridium thermocellum xylanase Z expressed in Escherichia coli and identification of the corresponding product in the culture medium of C. thermocellum. J. Bacteriol. 170:4576-4581.
23. Hayashi, H., M. Takehara, T. Hattori, T. Kimura, S. Karita, K. Sakka, and K. Ohmiya. 1999. Nucleotide sequences of two contiguous and highly homologous xylanase genes xynA and xynB and characterization of XynA from Clostridium thermocellum. Appl. Microbiol. Biotechnol. 51:348-357.
24. Kataeva, I., X.-L. Li, H. Chen, S.-K Choi, and L. G. Ljungdahl. 1999. Cloning and sequence analysis of a new cellulase gene encoding CelK, a major cellulosome component of Clostridium thermocellum: evidence for gene duplication and recombination. J. Bacteriol. 181:5288-5295.
25. Kirby, J., V. Aurilia, S. I. McCrae, J. C. Martin, and H. J. Flint. 1998. Plant cell wall degrading enzyme complexes from the cellulolytic rumen bacterium Ruminococcus flavefaciens. Biochem. Soc. Trans. 26:S169.
26. Kohring, S., J. Wiegel, and F. Mayer. 1990. Subunit composition and glycosidic activities of the cellulase complex from Clostridium thermocellum JW20. Appl. Environ. Microbiol. 56:3798-3804.
27. Koljak, R., O. Boutaud, B.-H. Shieh, N. Samel, and A. R. Brash. 1997. Identification of a naturally occurring peroxidase-lipoxygenase fusion protein. Science 277:1994-1996.
28. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
29. Lamed, R., E. Setter, and E. A. Bayer. 1983. Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum. J. Bacteriol. 156:828-836.
30. Lamed, R., E. Setter, R. Kenig, and E. A. Bayer. 1983. The cellulosome - a discrete cell surface organelle of Clostridium thermocellum which exhibits separate antigenic, cellulose-binding and various cellulolytic activities. Biotechnol. Bioeng. Symp. 13:163-181.
31. Laurie, J. I., J. H. Clarke, A. Ciruela, C. B. Faulds, G, Williamson, H. J. Gilbert, J. E. Rixon, J. Millward-Sadler, and G. P. Hazlewood. 1997. The NodB domain of a multidomain xylanase from Cellulomonas fimi deacetylates acetylxylan. FEMS Lett. 148:261-264.
32. Li, X.-L., H. Chen, and L. G. Ljungdahl. 1997. Two cellulases, CelA and CelC, from the polycentric anaerobic fungus Orpinomyces strain PC-2 contain N-terminal docking domains for a cellulase-hemicellulase complex. Appl. Environ. Microbiol. 63:4721-4728.
33. Ljungdahl, L. G., M. P. Coughlan, F. Mayer, Y. Mori, and K. Hon-nami. 1988. Macrocellulase complexes and yellow affinity substance from Clostridium thermocellum. Methods Enzymol. 160:483-500.
34. Ljungdahl, L. G., W. E. O'Brien, M. R. Moore, and M.-T. Liu. 1980. Methylene-tetrahydrofolate dehydrogenase from Clostridium formicoaceticum and methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase (combined) from Clostridium thermoaceticum. Methods Enzymol. 66:599-609.
35. McSweeney, C. S., A. Dulieu, and R. Bunch. 1998. Butyrivibrio spp. and other xylanolytic microorganisms from the rumen have cinnamoyl esterase activity. Anaerobe 4:57-65.
36. McSweeney, C. S., A. Dulieu, Y. Katayama, and J. B. Lowry. 1994. Solubilization of lignin by the ruminal anaerobic fungus Neocallimastix patriciarum. Appl. Environ. Microbiol. 60:2985-2989.
37. Miller, G. L. 1959. Use of dinitrosaliclic acid reagent for determination of reducing sugars. Anal. Chem. 31:127-132.
38. Morag, E., E. A. Bayer, and R. Lamed. 1992. Affinity digestion for the near-total recovery of purified cellulosome from Clostridium thermocellum. Enzyme Microb. Technol. 14:289-292.
39. Ralph, J., J. H. Grabber, and R. D. Hatfield. 1995. Lignin-ferulate cross-links in grasses: active incorporation of ferulate polysaccharides esters into ryegrass lignins. Carbohydr. Res. 275:167-178.
40. Selinger, L. B., C. W. Forsberg, and K.-J. Cheng. 1996. The rumen: a unique source of enzymes for enhancing livestock production. Anaerobe 2:263-284.
41. 1-tetrahydrofolate synthase. J. Biol. Chem. 263:7717-7725.
42. Souchon, H., S. Spinelli, P. Béguin, and P. M. Alzari. 1994. Crystallization and preliminary diffraction analysis of the catalytic domain of xylanase Z from Clostridium thermocellum. J. Mol. Biol. 235:1348-1350.
43. Stark, G. R. 1977. Multifunctional proteins: one gene - more than one enzyme. Trends Biochem. Sci. 2:64-66.
44. Williamson, G., P. A. Kroon, and C. B. Faulds. 1998. Hairy plant polysaccharides: a close shave with microbial esterases. Microbiology 144:2011-2023.