Toward improvement of erythromycin a production in an industrial Saccharopolyspora erythraea strain via facilitation of genetic manipulation with an artificial attB site for specific recombination

Applied and Environmental Microbiology

Volumn 77, Issue 21, 2011, Pages 7508-7516

  Wu, Jiequn ^a,b   Zhang, Qinglin ^a,b   Deng, Wei ^b   Qian, Jiangchao ^a   Zhang, Siliang ^a   Liu, Wen ^b  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b SHANGHAI INSTITUTE OF ORGANIC CHEMISTRY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ENDOGENOUS GENES; ERYTHROMYCIN A; GENETIC MANIPULATIONS; LARGE-SCALE PRODUCTION; LATE STAGE; S-ADENOSYLMETHIONINE SYNTHETASE; SACCHAROPOLYSPORA ERYTHRAEA; SITE-SPECIFIC ENGINEERING;

BIOCHEMISTRY; DNA SEQUENCES; DRUG PRODUCTS; GENES; INDUSTRY; OXYGEN SUPPLY; STAGES;

ERBIUM;

BACTERIOPHAGE RECEPTOR; ERYTHROMYCIN;

ANTIBIOTICS; BACTERIUM; BIOTRANSFORMATION; CRYPSIS; ENZYME ACTIVITY; FERMENTATION; GENETIC VARIATION; OXYGEN; RECOMBINATION;

ARTICLE; BACTERIUM CONJUGATION; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENETICS; METABOLISM; METHODOLOGY; MICROBIAL GENETICS; SACCHAROPOLYSPORA; TRANSGENIC ORGANISM;

ATTACHMENT SITES, MICROBIOLOGICAL; CONJUGATION, GENETIC; ERYTHROMYCIN; GENETIC ENGINEERING; GENETICS, MICROBIAL; METABOLIC NETWORKS AND PATHWAYS; ORGANISMS, GENETICALLY MODIFIED; RECOMBINATION, GENETIC; SACCHAROPOLYSPORA;

EID: 83155163762     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.06034-11     Document Type: Article

References (39)

1. Adrio, J. L., and A. L. Demain. 2006. Genetic improvement of processes yielding microbial products. FEMS Microbiol. Rev. 30:187-214.
2. Baltz, R. H. 2006. Molecular engineering approaches to peptide, polyketide and other antibiotics. Nat. Biotechnol. 24:1533-1540.
3. Baltz, R. H. 2010. Streptomyces and Saccharopolyspora hosts for heterologous expression of secondary metabolite gene clusters. J. Ind. Microbiol. Biotechnol. 37:759-772.
4. Bierman, M., et al. 1992. Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116:43-49.
5. Brunker, P., W. Minas, P. T. Kallio, and J. E. Bailey. 1998. Genetic engineering of an industrial strain of Saccharopolyspora erythraea for stable expression of the Vitreoscilla haemoglobin gene (vhb). Microbiology 144(Pt 9):2441-2448.
6. Butler, M. S. 2008. Natural products to drugs: natural product-derived compounds in clinical trials. Nat. Prod. Rep. 25:475-516.
7. Chen, Y., et al. 2008. Genetic modulation of the overexpression of tailoring genes eryK and eryG leading to the improvement of erythromycin A purity and production in Saccharopolyspora erythraea fermentation. Appl. Environ. Microbiol. 74:1820-1828.
8. Combes, P., R. Till, S. Bee, and M. C. Smith. 2002. The Streptomyces genome contains multiple pseudo-attB sites for the (phi)C31-encoded site-specific recombination system. J. Bacteriol. 184:5746-5752.
9. Deubel, A., and U. Holzgrabe. 2007. Development of an enhanced separation of erythromycin and its related substances by liquid chromatography. J. Pharm. Biomed. Anal. 43:493-498.
10. Fischbach, M. A., and C. T. Walsh. 2006. Biochemistry directing biosynthesis. Science 314:603-605.
11. Gupta, M., R. Till, and M. C. Smith. 2007. Sequences in attB that affect the ability of phiC31 integrase to synapse and to activate DNA cleavage. Nucleic Acids Res. 35:3407-3419.
12. Haydock, S. F., et al. 1991. Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases. Mol. Gen. Genet. 230:120-128.
13. Holmberg, N., G. Lilius, J. E. Bailey, and L. Bulow. 1997. Transgenic tobacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered metabolite production. Nat. Biotechnol. 15:244-247.
14. Kallio, P. T., and J. E. Bailey. 1996. Intracellular expression of Vitreoscilla hemoglobin (VHb) enhances total protein secretion and improves the production of alpha-amylase and neutral protease in Bacillus subtilis. Biotechnol. Prog. 12:31-39.
15. Katz, L., and G. W. Ashley. 2005. Translation and protein synthesis: macrolides. Chem. Rev. 105:499-528.
16. Khosla, C., and J. E. Bailey. 1988. Heterologous expression of a bacterial haemoglobin improves the growth properties of recombinant Escherichia coli. Nature 331:633-635.
17. Khosla, C., and J. E. Bailey. 1988. The Vitreoscilla hemoglobin gene: molecular cloning, nucleotide sequence and genetic expression in Escherichia coli. Mol. Gen. Genet. 214:158-161.
18. Kieser, T., M. J. Bibb, M. J. Buttner, K. F. Chater, and D. A. Howood. 2000. Practical Streptomyces genetics. John Innes Foundation, Norwich, United Kingdom.
19. Kim, D. J., et al. 2003. Accumulation of S-adenosyl-L-methionine enhances production of actinorhodin but inhibits sporulation in Streptomyces lividans TK23. J. Bacteriol. 185:592-600.
20. Labeda, D. P. 1987. Transfer of the type strain of Streptomyces erythraeus (Waksman 1923) Waksman and Henrici 1948 to the genus Saccharopolyspora Lacey and Goodfellow 1975 as Saccharopolyspora erythraea sp. nov., and designation of a neotype strain for Streptomyces erythraeus. Int. J. Syst. Bacteriol. 37:19-22.
21. Lambalot, R. H., D. E. Cane, J. J. Aparicio, and L. Katz. 1995. Overproduction and characterization of the erythromycin C-12 hydroxylase, EryK. Biochemistry 34:1858-1866.
22. Liu, W., and B. Shen. 2000. Genes for production of the enediyne antitumor antibiotic C-1027 in Streptomyces globisporus are clustered with the cagA gene that encodes the C-1027 apoprotein. Antimicrob. Agents Chemother. 44:382-392.
23. Minas, W., P. Brunker, P. T. Kallio, and J. E. Bailey. 1998. Improved erythromycin production in a genetically engineered industrial strain of Saccharopolyspora erythraea. Biotechnol. Prog. 14:561-566.
24. Okamoto, S., A. Lezhava, T. Hosaka, Y. Okamoto-Hosoya, and K. Ochi. 2003. Enhanced expression of S-adenosylmethionine synthetase causes overproduction of actinorhodin in Streptomyces coelicolor A3(2). J. Bacteriol. 185:601-609.
25. Oliynyk, M., et al. 2007. Complete genome sequence of the erythromycinproducing bacterium Saccharopolyspora erythraea NRRL23338. Nat. Biotechnol. 25:447-453.
26. Parekh, S., V. A. Vinci, and R. J. Strobel. 2000. Improvement of microbial strains and fermentation processes. Appl. Microbiol. Biotechnol. 54:287-301.
27. Paulus, T. J., et al. 1990. Mutation and cloning of eryG, the structural gene for erythromycin O-methyltransferase from Saccharopolyspora erythraea, and expression of eryG in Escherichia coli. J. Bacteriol. 172:2541-2546.
28. Pendse, G. J., and J. E. Bailey. 1994. Effect of Vitreoscilla hemoglobin expression on growth and specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells. Biotechnol. Bioeng. 44:1367-1370.
29. Reeves, A. R., et al. 2006. Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea. J. Ind. Microbiol. Biotechnol. 33:600-609.
30. Reeves, A. R., et al. 2007. Engineering of the methylmalonyl-CoA metabolite node of Saccharopolyspora erythraea for increased erythromycin production. Metab. Eng. 9:293-303.
31. Rodriguez, E., et al. 2003. Rapid engineering of polyketide overproduction by gene transfer to industrially optimized strains. J. Ind. Microbiol. Biotechnol. 30:480-488.
32. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
33. Stassi, D., S. Donadio, M. J. Staver, and L. Katz. 1993. Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. J. Bacteriol. 175:182-189.
34. Staunton, J., and K. J. Weissman. 2001. Polyketide biosynthesis: a millennium review. Nat. Prod. Rep. 18:380-416.
35. Thorpe, H. M., and M. C. Smith. 1998. In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family. Proc. Natl. Acad. Sci. U. S. A. 95:5505-5510.
36. Tsuji, K., and J. F. Goetz. 1978. HPLC as a rapid means of monitoring erythromycin and tetracycline fermentation processes. J. Antibiot. (Tokyo) 31:302-308.
37. Walsh, C. T., and M. A. Fischbach. 2010. Natural products version 2.0: connecting genes to molecules. J. Am. Chem. Soc. 132:2469-2493.
38. Wang, Y., J. Chu, Y. Zhuang, L. Zhang, and S. Zhang. 2007. Improved production of erythromycin A by expression of a heterologous gene encoding S-adenosylmethionine synthetase. Appl. Microbiol. Biotechnol. 75:837-842.
39. Weissman, K. J., and P. F. Leadlay. 2005. Combinatorial biosynthesis of reduced polyketides. Nat. Rev. Microbiol. 3:925-936