Intracellular expression of Vitreoscilla hemoglobin (VHb) enhances total protein secretion and improves the production of α-amylase and neutral protease in Bacillus subtilis

Biotechnology Progress

Volumn 12, Issue 1, 1996, Pages 31-39

  Kallio, Pauli T ^a   Bailey, James E ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; BATCH CELL CULTURE; BIOASSAY; BIOREACTORS; CELLS; ENZYME KINETICS; ENZYMES; GENES; GROWTH KINETICS;

ACETATE; AMYLASE ACTIVITY; BACILLUS SUBTILIS; BACTERIAL HEMOGLOBIN GENE; CARBON MONOXIDE BINDING ASSAY; IMMUNOBLOT ANALYSIS; INTRACELLULAR EXPRESSION; PROTEASE ACTIVITY; TOTAL PROTEIN SECRETION; VITREOSCILLA;

HEMOGLOBIN;

AMYLASE; BACILLOLYSIN; BACTERIAL PROTEIN; HEMOGLOBIN; HEMOGLOBIN PROTEIN, VITREOSCILLA; METALLOPROTEINASE; RECOMBINANT DNA; RECOMBINANT PROTEIN;

AEROBIC METABOLISM; ARTICLE; BACILLUS SUBTILIS; BIOSYNTHESIS; ESCHERICHIA COLI; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; WESTERN BLOTTING;

AEROBIOSIS; ALPHA-AMYLASE; BACILLUS SUBTILIS; BACTERIAL PROTEINS; BASE SEQUENCE; BLOTTING, WESTERN; CLONING, MOLECULAR; DNA, RECOMBINANT; ESCHERICHIA COLI; HEMOGLOBINS; METALLOENDOPEPTIDASES; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT PROTEINS;

EID: 0030038319     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp950065j     Document Type: Article

References (60)

1. Anagnostopoulos, C.; Spizizen, J. Requirements for transformation in Bacillus subtilis. J. Bacteriol. 1961, 169, 324-333.
2. Arbige, M. V.; Bulthuis, B. A.; Schultz, J.; Crabb, D. Fermentation of Bacillus. In Bacillus subtilis and other gram-positive bacteria; Sonnenshein, A. L., Hoch, J. A., Losick, R., Eds.; American Society for Microbiology: Washington, DC, 1993; pp 871-895.
3. Bailey, J. E. Host-vector interactions in Escherichia coli. Adv. Biochem. Eng./Biotechnol. 1993, 48, 29-52.
4. Band, L.; Henner, D. J. Bacillus subtilis requires a "stringent" Shine-Dalgarno region for gene expression. DNA 1984, 3, 17-21.
5. Bentley, W. E.; Davis, R. H.; Kompala, D. S. Dynamics of induced CAT expression in E. coli. Biotechnol. Bioeng. 1991, 38, 749-760.
6. Bernhard, K.; Utz, R. Production of Bacillus thuringiensis insecticides for experimental and commercial uses. In Bacillus thuringiensis, an environmental biopesticide: Theory and practice; Entwistle, P. F., Cory, J. S., Bailey, M. J., Higgs, S., Eds.; John Wiley & Sons Ltd: England, 1993; pp 255-267.
7. Borchert, T. V.; Nagarajan, V. Effect of signal sequence alteration on expression of levansucrase in Bacillus subtilis. J. Bacteriol. 1991, 173, 276-282.
8. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72, 248-254.
9. Burbulys, D.; Trach, K. A.; Hoch, J. A. The initiation of sporulation in Bacillus subtilis is controlled by a multicomponent phosphorelay. Cell 1991, 64, 545-552.
10. 31P NMR and saturation transfer study. Biotechnol. Prog. 1994, 10, 360-364.
11. Chen, W.; Hughes, D. E.; Bailey, J. E. Intracellular expression of Vitreoscilla hemoglobin alters aerobic metabolism of Saccharomyces cerevisiae. Biotechnol. Prog. 1994, 10, 308-313.
12. Chesnut, R. S.; Bookstein, C.; Hulett, F. M. Separate promoters direct expression of phoAIII, a member of the Bacillus subtilis alkaline phosphatase multigene family, during phosphate starvation and sporulation. Mol. Microbiol. 1991, 5, 2181-2190.
13. Dancer, B. N.; Mandelstam, J. Production and possible function of serine protease during sporulation of Bacillus subtilis. J. Bacteriol. 1975, 121, 406-410.
14. Demain, A. L. Production of nucleotides by microorganisms. In Economic microbiology: Primary products of metabolism; Rose, A.-H., Ed.; Academic Press: New York, 1978; Vol. 2, pp 187-208.
15. DeModena, J. A.; Gutiérrez, S.; Velasco, J.; Fernández, F. J.; Fachini, R. A.; Galazzo, J. L.; Hughes, D. E.; Martín, J. F. The production of cephalosporin C by Acremonium chrysogenum is improved by the intracellular expression of a bacterial hemoglobin. Bio/Technology 1993, 11, 926-929.
16. Dikshit, K. L.; Dikshit, R. P.; Webster, D. A. Study of Vitreoscilla globin (vgb) gene expression and promoter activity in E. coli through transcription fusion. Nucleic Acid Res. 1990, 18, 4149-4155.
17. Ferrari, E.; Jarnagin, A. S.; Schmidt, B. F. Commercial production of extracellular enzymes. In Bacillus subtilis and other gram-positive bacteria; Sonnenshein, A. L., Hoch, J. A., Losick, R., Eds.; American Society for Microbiology: Washington, DC, 1993; pp 917-937.
18. Freudl, R. Protein secretion in gram-positive bacteria. J. Biotechnol. 1992, 23, 231-240.
19. Haima, P.; Bron, S.; Venema, G. The effect of restriction shotgun cloning and plasmid stability in Bacillus subtilis Marburg. Mol. Gen. Genet. 1987, 209, 335-342.
20. Haima, P.; Van Sinderen, D.; Schotting, H.; Bron, S.; Venema, G. Development of a β-galactosidase α-complementation system for molecular cloning in Bacillus subtilis. Gene 1990, 86, 63-69.
21. Henner, D. J. Inducible expression of regulatory genes in Bacillus subtilis. Methods Enzymol. 1990, 185, 223-228.
22. Honjo, M.; Akaoka, A.; Nakayama, A.; Shimada, H.; Furutani, Y. Construction of a secretion vector containing the preprostructure coding region of Bacillus amyloliquefaciens neutral protease gene and secretion of Bacillus subtilis α-amylase and human interferon-beta in Bacillus subtilis. J. Biotechnol. 1985, 3, 73-84.
23. Kallio, P. The effect of the inverted repeat structure on the production of the cloned Bacillus amyloliquefaciens α-amylase. Eur. J. Biochem. 1986, 158, 491-495.
24. Kallio, P.; Palva, A.; Palva, I. Enhancement of α-amylase production by integrating and amplifying the α-amylase gene of Bacillus amyloliquefaciens in the genome of Bacillus subtilis. Appl. Microbiol. Biotechnol. 1987, 27, 64-71.
25. Kallio, P. T.; Fagelson, J. E.; Hoch, J. A.; Strauch, M. A. The transition state regulator Hpr of Bacillus subtilis is a DNA-binding protein. J. Biol. Chem. 1991, 266, 13411-13417.
26. Kallio, P. T.; Kim, D. J.; Tsai, P. S.; Bailey, J. E. Intracellular expression of Vitreoscilla hemoglobin alters Escherichia coli energy metabolism under oxygen-limited conditions. Eur. J. Biochem. 1994, 219, 201-208.
27. Kapp, N. V.; Edwards, C. W.; Chesnut, R. S.; Hulett, F. M. The Bacillus subtilis phoAIV gene: effects of in vitro inactivation on total alkaline phosphatase production. Gene 1990, 96, 95-100.
28. Khosla, C.; Bailey, J. E. Heterologous expression of a bacterial haemoglobin improves the growth properties of recombinant Escherichia coli. Nature 1988, 331, 633-635.
29. Khosla, C.; Curtis, J. E.; DeModena, J.; Rinas, U.; Bailey, J. E. Expression of intracellular hemoglobin improves protein synthesis in oxygen-limited Escherichia coli. Bio/Technology 1990, 8, 849-853.
30. Khosravi, M.; Webster, D. A.; Stark, B. C. Presence of the bacterial hemoglobin gene improves α-amylase production of a recombinant Escherichia coli strain. Plasmid 1990, 24, 190-194.
31. Kleinkauf, H.; von Dohren, H, Non-ribosomal peptide formation on multifunctional proteins. Trends Biochem. Sci. 1983, 8, 281-283.
32. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680-685.
33. Laureus, M.; Haltia, T.; Saraste, M.; Wikström, M. Bacillus subtilis expresses two kinds of haem A-containing terminal oxidases. Eur. J. Biochem. 1991, 197, 699-705.
34. Lennox, E. S. Transduction of linked genetic characters of the host by bacteriophage P1. Virology 1955, 1, 190-206.
35. Magnolo, S. K.; Leenutaphong, D. L.; DeModena, J. A.; Curtis, J. E.; Bailey, J. E.; Galazzo, J. L.; Hughes, D. E. Actinorhodin production by Streptomyces coelicolor and growth of Streptomyces lividans are improved by expression of a bacterial hemoglobin. Bio/Technology 1991, 9, 473-476.
36. Moran, C. P., Jr.; Lang, N.; LeGrice, S. F. J.; Lee, G.; Stephens, M.; Sonnenshein, A. L.; Pero, J.; Losick, R. Nucleotide sequences that signal the initiation of transcription and translation in Bacillus subtilis. Mol. Gen. Genet. 1982, 186, 339-346.
37. Muren, E. M.; Randall, L. Export of α-amylase by Bacillus amyloliquefaciens requires proton motive force. J. Bacteriol. 1985, 164, 712-716.
38. Nagarajan, V. Protein secretion. In Bacillus subtilis and other gram-positive bacteria; Sonnenshein, A. L., Hoch., J. A., Losick, R., Eds.; American Society for Microbiology: Washington, DC, 1993; pp 713-726.
39. Nagarajan, V.; Thompson, L. D. Secretion of a heterologous protein from Bacillus subtilis with the aid of protease signal sequences. J. Bacteriol. 1986, 165, 837-842.
40. Ohmura, K.; Shiroza, T.; Nakamura, K.; Nakayama, A.; Yamane, K.; Yoda, K.; Yamasaki, M.; Tamura, G. A. Bacillus subtilis secretion vector system derived from the B. subtilis α-amylase promoter and signal sequence region and secretion of Escherichia coli β-lactamase by the vector system. J. Biochem. 1984, 95, 87-93.
41. Orii, Y.; Webster, D. A. Photodissociation of oxygenated cytochrome o(s) (Vitreoscilla) and kinetic studies of reassociation. J. Biol. Chem. 1986, 261, 3544-3547.
42. Pendse, G. J.; Bailey, J. E. Effect of Vitreoscilla hemoglobin expression on growth an specific tissue plasminogen activator productivity in recombinant Chinese hamster ovary cells. Biotechnol. Bioeng. 1994, 44, 1367-1370.
43. Priest, F. G. Extracellular enzyme synthesis in genus Bacillus. Bacteriol. Rev. 1977, 41, 711-735.
44. Saiki, R. K.; Gelfand, D. H.; Stoffel, S.; Scharf, S. J.; Higuchi, R.; Horn, G. T.; Mullis, K. B.; Erlich, H. A. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988, 239, 487-491.
45. Sambrook, J.; Fritsch, E. F.; Maniatis, T. Molecular cloning: a laboratory manual, 2nd ed.; Cold Spring Harbor Laboratory: Cold Spring Harbor, NY, 1989.
46. Sander, F. C.; Fachini, R. A.; Hughes, D. E.; Galazzo, J. L.; Bailey, J. E. Expression of Vitreoscilla hemoglobin in Corynebacterium glutamicum increases final concentration and yield of L-lysine. In Progress in Biotechnology. ECB6:Proceedings of the 6th European Congress on Biotechnology; Alberghina, L., Frontali, L., Sensi P., Eds.; Elsevier Science B. V.: Amsterdam, 1994; Vol. 6, pp 607-610.
47. Sanger, F.; Nicklen, S.; Caulson, A. R. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 1977, 74, 5463-5467.
48. Saunders, C. W.; Pedroni, J. A.; Monahan, P. Secretion from B. subtilis of a 34 amino acid residue fragment of human parathyroid hormone: effect of sequences adjacent to the signal sequence cleavage. Gene 1991, 102, 277-282.
49. Schaeffer, P.; Millet, J.; Aubert, J. D. Catabolite repression of bacterial sporulation. Proc. Natl. Acad. Sci. U.S.A. 1965, 54, 704-711.
50. Schein, C. H.; Kashiwagi, K.; Fujisawa, A.; Weissman, C. Secretion of mature IFN-α2 and accumulation of uncleaved precursor by Bacillus subtilis transformed with a hybrid α-amylase signal sequence-IFN-α2 gene. Bio/Technology 1986, 4, 719-725.
51. Seressiotis, A.; Bailey, J. E. Optimal gene expression and amplification strategies for batch and continuous recombinant cultures. Biotechnol. Bioeng. 1987, 29, 392-398.
52. Simonen, M.; Palva, I. Protein secretion in Bacillus species. Microbiol. Rev. 1993, 57, 109-137.
53. Smith, I. Regulatory proteins that control late-growth development. In Bacillus subtilis and other gram-positive bacteria; Sonnenshein, A. L., Hoch, J. A., Losick, R., Eds.; American Society for Microbiology: Washington, DC, 1993; pp 785-800.
54. Trach, K.; Burbulys, D.; Strauch, M.; Wu, J.-J.; Dhillon, N.; Jonas, R.; Hanstein, C.; Kallio, P.; Perego, M.; Bird, T.; Spiegelman, G.; Fogher, C.; Hoch, J. A. Control of the initiation of sporulation in Bacillus subtilis by a phosphorelay. Res. Microbiol. 1991, 142, 815-823.
55. 3-type oxidases. Mol. Microbiol. 1991, 5, 2063-2072.
56. Vehmaanperä, J.; Nybergh, P. M. A.; Tanner, R.; Pohjonen, E.; Bergelin, R.; Korhola, M. Industrial production of α-amylase by genetically engineered Bacillus. Enzyme Microb. Technol. 1987, 9, 546-549.
57. Webster, D. A.; Liu, C. Y. Reduced nicotinaminde adenine dinucleotide cytochrome o reductase associated with cytochrome o purified from Vitreoscilla. J. Biol. Chem. 1974, 249, 4257-4260.
58. Winston, S. E.; Fuller, S. A.; Hurrell, J. G. R. Western blotting. In Current protocols in molecular biology; Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., Strahl, K., Eds.; John Wiley & Sons, Inc.: New York, 1987; pp 10.8.1-10.8.6.
59. Wong, S.-L.; Doi, R. H. Determination of the signal peptidase cleavage site in the preprosubtilisin of Bacillus subtilis. J. Biol. Chem. 1986, 261, 10176-10181.
60. Yansura, D. G.; Henner, D. J. Use of Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis. Proc. Natl. Acad. Sci. U.S.A. 1984, 81, 439-443.