Genetic modulation of the overexpression of tailoring genes eryK and eryG leading to the improvement of erythromycin A purity and production in Saccharopolyspora erythraea fermentation

Applied and Environmental Microbiology

Volumn 74, Issue 6, 2008, Pages 1820-1828

  Chen, Yun ^a,b   Deng, Wei ^b   Wu, Jiequn ^a,b   Qian, Jiangchao ^a   Chu, Ju ^a   Zhuang, Yingping ^a   Zhang, Siliang ^a   Liu, Wen ^b  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b SHANGHAI INSTITUTE OF ORGANIC CHEMISTRY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BIOTRANSFORMATION; HOMOLOGOUS RECOMBINATION;

BIOSYNTHESIS; FERMENTATION; MICROBIOLOGY;

GENE EXPRESSION;

BERYTHROMYCIN; ERYTHROMYCIN; ERYTHROMYCIN C; METHYLTRANSFERASE; OXYGENASE; PROTEIN ERYG; PROTEIN ERYK; UNCLASSIFIED DRUG;

BACTERIUM; BIOTRANSFORMATION; COST-BENEFIT ANALYSIS; FERMENTATION; GENE EXPRESSION; GENETIC ENGINEERING; RECOMBINATION;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENE; BACTERIAL STRAIN; BIOTRANSFORMATION; ENZYME ACTIVITY; ERYG GENE; ERYK GENE; FERMENTATION; GENE CONTROL; GENE LOCUS; GENE NUMBER; GENE OVEREXPRESSION; GENETIC ENGINEERING; GENOTYPE; NONHUMAN; NUCLEOTIDE SEQUENCE; SACCHAROPOLYSPORA; STREPTOMYCES ERYTHRAEUS;

BACTERIAL PROTEINS; CYTOCHROME P-450 ENZYME SYSTEM; ERYTHROMYCIN; FERMENTATION; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; METHYLTRANSFERASES; MIXED FUNCTION OXYGENASES; MOLECULAR STRUCTURE; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SACCHAROPOLYSPORA;

SACCHAROPOLYSPORA ERYTHRAEA;

EID: 40849100148     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02770-07     Document Type: Article

References (33)

1. Adrio, J. L., and A. L. Demain. 2006. Genetic improvement of processes yielding microbial products. FEMS Microbiol. Rev. 30:187-214.
2. Baltz, R. H. 2006. Molecular engineering approaches to peptide, polyketide and other antibiotics. Nat. Biotechnol. 24:1533-1540.
3. Bierman, M., R. Logan, K. O'Brien, E. T. Seno, R. N. Rao, and B. E. Schoner. 1992. Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene 116:43-49.
4. Brunker, P., W. Minas, P. T. Kallio, and J. E. Bailey. 1998. Genetic engineering of an industrial strain of Saccharopolyspora erythraea for stable expression of the Vitreoscilla haemoglobin gene (vhb). Microbiology 144: 2441-2448.
5. Cropp, A., S. Chen, H. Liu, W. Zhang, and K. A. Reynolds. 2001. Genetic approaches for controlling ratios of related polyketide products in fermentation processes. J. Ind. Microbiol. Biotechnol. 27:368-377.
6. Desai, R. P., E. Rodriguez, J. L. Galazzo, and P. Licari. 2004. Improved bioconversion of 15-fluoro-6-deoxyerythronolides B to 15-fluoro-erythromycin A by overexpression of the eryK gene in Saccharopolyspora erythraea. Biotechnol. Prog. 20:1660-1665.
7. Deubel, A., and U. Holzgrabe. 2007. Development of an enhanced separation of erythromycin and its related substances by liquid chromatography. J. Pharm. Biomed. Anal. 43:493-498.
8. Douthwaite, S., and W. S. Champney. 2001. Structures of ketolides and macrolides determine their mode of interaction with the ribosomal target site. J. Antimicrob. Chemother. 48(Suppl. T1):1-8.
9. European Directorate for the Quality of Medicines and Healthcare. 1997. European pharmacopoeia, 3rd ed. European Directorate for the Quality of Medicines and Healthcare, Strasbourg, France.
10. Fischbach, M. A., and C. T. Walsh. 2006. Biochemistry. Directing biosynthesis. Science 314:603-605.
11. Haydock, S. F., J. A. Dowson, N. Dhillon, G. A. Roberts, J. Cortes, and P. F. Leadlay. 1991. Cloning and sequence analysis of genes involved in erythromycin biosynthesis in Saccharopolyspora erythraea: sequence similarities between EryG and a family of S-adenosylmethionine-dependent methyltransferases. Mol. Gen. Genet. 230:120-128.
12. Katz, L., and C. Khosla. 2007. Antibiotic production from the ground up. Nat. Biotechnol. 25:428-429.
13. Kieser, T., M. J. Bibb, M. J. Buttner, K. F. Chater, and D. A. Hopwood. 2000. Practical Streptomyces genetics. John Innes Foundation, Norwich, United Kingdom.
14. Labeda, D. P. 1987. Transfer of the type strain of Streptomyces erythraeus (Waksman 1923) Waksman and Henrici 1948 to the genus Saccharopolyspora Lacey and Goodfellow 1975 as Saccharopolyspora erythraea sp. nov., and designation of a neotype strain for Streptomyces erythraeus. Int. J. Syst. Bacteriol. 37:19-22.
15. Lambalot, R. H., D. E. Cane, J. J. Aparicio, and L. Katz. 1995. Overexpression and characterization of the erythromycin C-12 hydroxylase, EryK. Biochemistry 34:1858-1866.
16. Liu, W., and B. Shen. 2000. Genes for production of the enediyne antitumor antibiotic C-1027 in Streptomyces globisporus are clustered with the cagA gene that encodes the C-1027 apoprotein. Antimicrob. Agents Chemother. 44:382-392.
17. -ΔΔCT method. Methods 25: 402-408.
18. Minas, W., P. Brunker, P. T. Kallio, and J. E. Bailey. 1998. Improved erythromycin production in a genetically engineered industrial strain of Saccharopolyspora erythraea. Biotechnol. Prog. 14:561-566.
19. Oliynyk, M., M. Samborskyy, J. B. Lester, T. Mironenko, N. Scott, S. Dickens, S. F. Haydock, and P. F. Leadlay. 2007. Complete genome sequence of the erythromycin-producing bacterium Saccharopolyspora erythraea NRRL23338. Nat. Biotechnol. 25:447-453.
20. Parekh, S., V. A. Vinci, and R. J. Strobel. 2000. Improvement of microbial strains and fermentation processes. Appl. Microbiol. Biotechnol. 54:287-301.
21. Paulus, T. J., J. S. Tuan, V. E. Luebke, G. T. Maine, J. P. DeWitt, and L. Katz. 1990. Mutation and cloning of eryG, the structural gene for erythromycin O-methyltransferase from Saccharopolyspora erythraea, and expression of eryG in Escherichia coli. J. Bacteriol. 172:2541-2546.
22. Pfaffl, M. W. 2001. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29:e45.
23. Reeves, A. R., R. S. English, J. S. Lampel, D. A. Post, and T. J. Vanden Boom. 1999. Transcriptional organization of the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea. J. Bacteriol. 181:7098-7106.
24. Reeves, A. R., I. A. Brikun, W. H. Cernota, B. I. Leach, M. C. Gonzalez, and J. M. Weber. 2006. Effects of methylmalonyl-CoA mutase gene knockouts on erythromycin production in carbohydrate-based and oil-based fermentations of Saccharopolyspora erythraea. J. Ind. Microbiol. Biotechnol. 33:600-609.
25. Reeves, A. R., I. A. Brikun, W. H. Cernota, B. I. Leach, M. C. Gonzalez, and J. M. Weber. 2007. Engineering of the methylmalonyl-CoA metabolite node of Saccharopolyspora erythraea for increased erythromycin production. Metab. Eng. 9:293-303.
26. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
27. Stassi, D., S. Donadio, M. J. Staver, and L. Katz. 1993. Identification of a Saccharopolyspora erythraea gene required for the final hydroxylation step in erythromycin biosynthesis. J. Bacteriol. 175:182-189.
28. Staunton, J., and K. J. Weissman. 2001. Polyketide biosynthesis: a millennium review. Nat. Prod. Rep. 18:380-416.
29. Tsuji, K., and J. F. Goetz. 1978. HPLC as a rapid means of monitoring erythromycin and tetracycline fermentation processes. J. Antibiot. 31:302-308.
30. Vara, J., M. Lewandowska-Sharbek, Y. G. Wang, S. Donadio, and C. R. Hutchinson. 1989. Cloning of genes governing the deoxysugar portion of the erythromycin biosynthesis pathway in Saccharopolyspora erythraea (Streptomyces erythraea). J. Bacteriol. 171:5872-5881.
31. Wang, Y., Y. Wang, J. Chu, Y. Zhuang, L. Zhang, and S. Zhang. 2007. Improved production of erythromycin A by expression of a heterologous gene encoding 5-adenosylmethionine synthase. Appl. Microbiol. Biotechnol. 75:837-842.
32. Weissman, K. J., and P. F. Leadlay. 2005. Combinatorial biosynthesis of reduced polyketides. Nat. Rev. Microbiol. 3:925-936.
33. Woo, A. J., W. R. Strohl, and N. D. Priestley. 1999. Nonactin biosynthesis: the product of nonS catalyzes the formation of the furan ring of nonactic acid. Antimicrob. Agents Chemother. 43:1662-1668.