Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein-encoding mRNA

Nucleic Acids Research

Volumn 39, Issue 21, 2011, Pages

  Karikó, Katalin ^a   Muramatsu, Hiromi ^a   Ludwig, János ^b   Weissman, Drew ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b UNIVERSITY OF BONN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKINE; DOUBLE STRANDED RNA; INTERFERON; MESSENGER RNA; NUCLEOSIDE DERIVATIVE;

ARTICLE; CONTAMINATION; CONTROLLED STUDY; CYTOKINE RELEASE; GENE THERAPY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; HUMAN CELL; IMMUNOSTIMULATION; IN VITRO STUDY; INFLAMMATION; INNATE IMMUNITY; PRIORITY JOURNAL; PURIFICATION; RNA TRANSCRIPTION; RNA TRANSLATION; STEM CELL;

ANIMALS; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYTIDINE; GENE THERAPY; HEK293 CELLS; HUMANS; MICE; PROTEIN BIOSYNTHESIS; PSEUDOURIDINE; RNA, DOUBLE-STRANDED; RNA, MESSENGER; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 82255194361     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkr695     Document Type: Article

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