Preventing nondesired RNA-primed RNA extension catalyzed by T7 RNA polymerase

European Journal of Biochemistry

Volumn 270, Issue 7, 2003, Pages 1458-1465

  Nacheva, Genoveva A ^a,b   Berzal Herranz, Alfredo ^a  

^a INSTITUTO DE PARASITOLOGÍA Y BIOMEDICINA LÓPEZ NEYRA   (Spain)

^b INSTITUTE OF MOLECULAR BIOLOGY   (Bulgaria)

Author keywords

DNA template; In vitro transcription; RNA primed RNA extension; RNA primed template; T7 RNA polymerase

Indexed keywords

DOUBLE STRANDED DNA; PRIMER RNA; RNA POLYMERASE;

ARTICLE; BACTERIOPHAGE; BASE PAIRING; CATALYST; DNA TEMPLATE; DNA TRANSCRIPTION; IN VITRO STUDY; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; RNA ANALYSIS; RNA TRANSCRIPTION;

BASE PAIRING; CATALYSIS; CELL-FREE SYSTEM; DNA; DNA-DIRECTED RNA POLYMERASES; GENE LIBRARY; RNA; RNA, CATALYTIC; RNA, COMPLEMENTARY; SUBSTRATE SPECIFICITY; TEMPLATES, GENETIC; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

UNIDENTIFIED BACTERIOPHAGE;

EID: 0037392256     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1033.2003.03510.x     Document Type: Article

References (24)

1. Chamberlin, M. & Ryan, T. (1982) Bacteriophage DNA-dependent RNA polymerases. In The Enzymes, Vol. XV, (Boyer, P.D., ed.), pp. 87-108. Academic Press Inc., New York, USA.
2. Sousa, R., Debabrata, P. & Lafer, E.M. (1992) Model for the mechanism of bacteriophage T7 RNAP transcription initiation and termination. J. Mol. Biol. 224, 319-334.
3. Jia, Y. & Patel, S. (1997) Kinetic mechanism of transcription initiation by bacteriophage T7 RNA polymerase. Biochemistry 36, 4223-4232.
4. Cheetman, G.M. & Steitz, T.A. (1999) Structure of a transcribing T7 RNA Polymerase initiation complex. Science 286, 2305-2308.
5. Huang, J. & Sousa, R. (2000) T7 RNA polymerase elongation complex structure and movement. J. Mol. Biol. 303, 347-358.
6. Mentesana, P.E., Chin-Bow, S.T., Sousa, R. & McAllister, W.T. (2000) Characterization of halted T7 RNA polymerase elongation complexes reveals multiple factors that contribute to stability. J. Mol. Biol. 302, 1049-1062.
7. Milligan, J.F., Groebe, D.R., Witherell, G.W. & Uhlenbeck, O. (1987) Oligonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates. Nucleic Acids Res. 15, 8783-8798.
8. Arnaud-Barbe, N., Chaynet-Sauvion, V., Oriol, G., Mandrand, B. & Mallet, F. (1998) Transcription of RNA templates by T7 RNA polymerase. Nucleic Acids Res. 26, 3550-3554.
9. Konarska, M. & Sharp, P. (1989) Replication of RNA by the DNA-dependent RNA polymerase of phage T7. Cell 57, 423-431.
10. Konarska, M. & Sharp, P. (1990) Structure of RNAs replicated by the DNA-dependent T7 RNA polymerase. Cell 63, 609-618.
11. Bierbricher, C.K. & Luce, R. (1996) Template-free generation of RNA species that replicate with bacteriophage T7 RNA polymerase. EMBO J. 15, 3458-3465.
12. Cazanave, C. & Uhlenbeck, O. (1994) RNA template-directed RNA synthesis by T7 RNA polymerase. Proc. Natl Acad. Sci. USA 91, 6972-6976.
13. Triana-Alonso, F.J., Dabrowski, M., Wadzack, J. & Nierhaus, K.H. (1995) Self-coded 3′-extension of fun-off transcripts produces aberrant products during in vitro transcription with T7 RNA polymerase. J. Biol. Chem. 270, 6298-6307.
14. Krupp, G. (1989) Unusual promoter-independent transcription reactions with bacteriophage RNA polymerases. Nucleic Acids Res. 17, 3023-3036.
15. Daube, S.S. & von Hippel, P.H. (1994) RNA displacement pathway during transcription form synthetic RNA-DNA bubble duplex. Biochemistry 33, 340-347.
16. Davanloo, P., Rosenberg, A.H., Dunn, J.J. & Studier, F.W. (1984) Cloning and expression of the gene for bacteriophage T7 RNA polymerase. Proc. Natl Acad. Sci. USA 81, 2035-2039.
17. Kotani, H., Ishizaki, Y., Hiraoka, N. & Obayashi, A. (1987) Nucleotide sequence and expression of the cloned gene of bacteriophage SP6 RNA polymerase. Nucleic Acids Res. 15, 2653-2664.
18. Pérez-Ruiz, M., Barroso-delJesus, A. & Berzal-Herranz, A. (1999) Specificity of the hairpin ribozyme. J. Biol. Chem. 274, 29376-29380.
19. Milligan, J.F. & Uhlenbeck, O. (1989) Synthesis of small RNAs using T7 RNA polymerase. Methods Enzymol. 180, 51-62.
20. Pérez-Ruiz, M., Sievers, D., García-Lopez, P.A. & Berzal-Herranz, A. (1999) The antisense sequence of the HIV-1 TAR stem-loop structure covalently linked to the hairpin ribozyme enhances its catalytic activity against two artificial substrates. Antisense Nucl. Acid Drug Dev. 9, 33-42.
21. Barroso-delJesus, A., Tabler, M. & Berzal-Herranz, A. (1999) Comparative kinetic analysis of structural variants of the hairpin ribozyme reveals further potential to optimize its catalytic performance. Antisense Nucleic Acid Drug Development 9, 433-440.
22. Martin, C., Muller, D. & Coleman, J. (1988) Processivity in early stages of transcription by T7 RNA polymerase. Biochemistry 27, 3966-3974.
23. Moroney, S.E. & Piccirilli, J.A. (1991) Abortive products as initiating nucleotides during transcription by T7 RNA polymerase. Biochemistry 30, 10343-10349.
24. Donzé, O. & Picard, D. (2002) RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase. Nucleic Acids Res. 30, e46. http://nar.oupjournals.org/