Selective 13C labeling of nucleotides for large RNA NMR spectroscopy using an E. coli strain disabled in the TCA cycle

Journal of Biomolecular NMR

Volumn 48, Issue 4, 2010, Pages 179-192

  Thakur, Chandar S ^a   Sama, Jacob N ^a   Jackson, Melantha E ^a   Chen, Bin ^a   Dayie, T Kwaku ^a  

^a Bldg 142   (United States)

Author keywords

Alternate site specific labeling; CH2 methylene TROSY; Ribose and nucleobase; RNA; Structure and dynamics

Indexed keywords

CARBENE; CARBON 13; FORMIC ACID; GLYCEROL; MALATE DEHYDROGENASE; NUCLEOTIDE; PURINE; PYRIMIDINE; RIBOSE; RNA; SUCCINATE DEHYDROGENASE; TRICARBOXYLIC ACID; BACTERIAL RNA; CARBON;

ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BACTERIUM MUTANT; CARBON NUCLEAR MAGNETIC RESONANCE; CHEMICAL LABELING; CITRIC ACID CYCLE; CROSS COUPLING REACTION; CULTURE MEDIUM; ESCHERICHIA COLI; NONHUMAN; PRIORITY JOURNAL; PROTON TRANSPORT; RNA ANALYSIS; RNA STRUCTURE; BIOSYNTHESIS; CHEMISTRY; CONFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOTOPE LABELING; METABOLISM; METHODOLOGY; NUCLEAR MAGNETIC RESONANCE; OXIDATION REDUCTION REACTION; PENTOSE PHOSPHATE CYCLE;

ESCHERICHIA COLI;

CARBON ISOTOPES; CITRIC ACID CYCLE; ESCHERICHIA COLI; GLYCEROL; ISOTOPE LABELING; MALATE DEHYDROGENASE; MOLECULAR CONFORMATION; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; NUCLEIC ACID CONFORMATION; NUCLEOTIDES; OXIDATION-REDUCTION; PENTOSE PHOSPHATE PATHWAY; RIBOSE; RNA, BACTERIAL; SUCCINATE DEHYDROGENASE;

EID: 78651333567     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-010-9454-4     Document Type: Article

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