Preparative scale production of functional mouse aquaporin 4 using different cell-free expression modes

PLoS ONE

Volumn 5, Issue 9, 2010, Pages

  Kai, Lei ^a,b   Kaldenhoff, Ralf ^c   Lian, Jiazhang ^a   Zhu, Xiangcheng ^a   Dötsch, Volker ^b   Bernhard, Frank ^b   Cen, Peilin ^a   Xu, Zhinan ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b GOETHE UNIVERSITY   (Germany)

^c DARMSTADT UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AQUAPORIN 4; LIPOSOME; MEMBRANE PROTEIN;

ANIMAL CELL; ARTICLE; BIOTECHNOLOGICAL PRODUCTION; CELL FREE SYSTEM; CONTROLLED STUDY; ESCHERICHIA COLI; FILTRATION; MICELLE; MOUSE; NONHUMAN; PRECIPITATION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SCREENING TEST; WATER TRANSPORT; ANIMAL; CHEMISTRY; COMPARATIVE STUDY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING;

EUKARYOTA; MAMMALIA;

ANIMALS; AQUAPORIN 4; CELL-FREE SYSTEM; ESCHERICHIA COLI; GENE EXPRESSION; MEMBRANE PROTEINS; MICE; PROTEIN ENGINEERING;

EID: 77958544345     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0012972     Document Type: Article

References (45)

1. Borgnia MJ, Kozono D, Calamita G, Maloney PC, Agre P (1999) Functional reconstitution and characterization of AqpZ, the E. coli water channel protein. J Mol Biol 291: 1169-1179.
2. Lu MQ, Lee MD, Smith BL, Jung JS, Agre P, et al. (1996) The human AQP4 gene: Definition of the locus encoding two water channel polypeptides in brain. Proceedings of the National Academy of Sciences of the United States of America 93: 10908-10912.
3. Papadopoulos MC, Verkman AS (2008) Potential utility of aquaporin modulators for therapy of brain disorders. Prog Brain Res 170: 589-601.
4. Costa C, Tortosa R, Rodriguez A, Ferrer I, Torres JM, et al. (2007) Aquaporin 1 and aquaporin 4 overexpression in bovine spongiform encephalopathy in a transgenic murine model and in cattle field cases. Brain Res 1175: 96-106.
5. Misu T, Fujihara K, Kakita A, Konno H, Nakamura M, et al. (2007) Loss of aquaporin 4 in lesions of neuromyelitis optica: distinction from multiple sclerosis. Brain 130: 1224-1234.
6. Perez E, Barrachina M, Rodriguez A, Torrejon-Escribano B, Boada M, et al. (2007) Aquaporin expression in the cerebral cortex is increased at early stages of Alzheimer disease. Brain Res 1128: 164-174.
7. Badaut J, Ashwal S, Tone B, Regli L, Tian HR, et al. (2007) Temporal and regional evolution of aquaporin-4 expression and magnetic resonance imaging in a rat pup model of neonatal stroke. Pediatr Res 62: 248-254.
8. Hiroaki Y, Tani K, Kamegawa A, Gyobu N, Nishikawa K, et al. (2006) Implications of the aquaporin-4 structure on array formation and cell adhesion. J Mol Biol 355: 628-639.
9. Swartz J (2006) Developing cell-free biology for industrial applications. J Ind Microbiol Biotechnol 33: 476-485.
10. Xu Z, Lian J, Cai J (2010) Efficient Expression of Aquaporin Z in Escherichia coli Cell-free System Using Different Fusion Vectors. Protein Pept Lett 17: 181-185.
11. Lian J, Fang X, Cai J, Chen Q, Zheng Q, et al. (2008) Efficient expression of membrane-bound water channel protein (Aquaporin Z) in Escherichia coli. Protein Pept Lett 15: 687-691.
12. Winnick T (1950) Studies on the mechanism of protein synthesis in embryonic and tumor tissues. II. Inactivation of fetal rat liver homogenates by dialysis, and reactivation by the adenylic acid system. Arch Biochem 28: 338-347.
13. Winnick T (1950) Studies on the mechanism of protein synthesis in embryonic and tumor tissues. I. Evidence relating to the incorporation of labeled amino acids into protein structure in homogenates. Arch Biochem 27: 65-74.
14. Borsook H (1950) Protein turnover and incorporation of labeled amino acids into tissue proteins in vivo and in vitro. Physiol Rev 30: 206-219.
15. Klammt C, Schwarz D, Lohr F, Schneider B, Dotsch V, et al. (2006) Cell-free expression as an emerging technique for the large scale production of integral membrane protein. FEBS J 273: 4141-4153.
16. Berrier C, Park KH, Abes S, Bibonne A, Betton JM, et al. (2004) Cell-free synthesis of a functional ion channel in the absence of a membrane and in the presence of detergent. Biochemistry 43: 12585-12591.
17. Elbaz Y, Steiner-Mordoch S, Danieli T, Schuldiner S (2004) In vitro synthesis of fully functional EmrE, a multidrug transporter, and study of its oligomeric state. Proc Natl Acad Sci U S A 101: 1519-1524.
18. Klammt C, Lohr F, Schafer B, Haase W, Dotsch V, et al. (2004) High level cellfree expression and specific labeling of integral membrane proteins. Eur J Biochem 271: 568-580.
19. Klammt C, Schwarz D, Fendler K, Haase W, Dotsch V, et al. (2005) Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system. FEBS J 272: 6024-6038.
20. Keller T, Schwarz D, Bernhard F, Dotsch V, Hunte C, et al. (2008) Cell free expression and functional reconstitution of eukaryotic drug transporters. Biochemistry 47: 4552-4564.
21. Schwarz D, Klammt C, Koglin A, Lohr F, Schneider B, et al. (2007) Preparative scale cell-free expression systems: new tools for the large scale preparation of integral membrane proteins for functional and structural studies. Methods 41: 355-369.
22. Klammt C, Schwarz D, Eifler N, Engel A, Piehler J, et al. (2007) Cell-free production of G protein-coupled receptors for functional and structural studies. J Struct Biol 158: 482-493.
23. Jung JS, Bhat RV, Preston GM, Guggino WB, Baraban JM, et al. (1994) Molecular characterization of an aquaporin cDNA from brain: candidate osmoreceptor and regulator of water balance. Proc Natl Acad Sci U S A 91: 13052-13056.
24. Neely JD, Christensen BM, Nielsen S, Agre P (1999) Heterotetrameric composition of aquaporin-4 water channels. Biochemistry 38: 11156-11163.
25. Zeidel ML, Nielsen S, Smith BL, Ambudkar SV, Maunsbach AB, et al. (1994) Ultrastructure, pharmacologic inhibition, and transport selectivity of aquaporin channel-forming integral protein in proteoliposomes. Biochemistry 33: 1606-1615.
26. Yukutake Y, Tsuji S, Hirano Y, Adachi T, Takahashi T, et al. (2008) Mercury chloride decreases the water permeability of aquaporin-4-reconstituted proteoliposomes. Biol Cell 100: 355-363.
27. Rojek A, Praetorius J, Frokiaer J, Nielsen S, Fenton RA (2008) A current view of the mammalian aquaglyceroporins. Annu Rev Physiol 70: 301-327.
28. Manley GT, Fujimura M, Ma T, Noshita N, Filiz F, et al. (2000) Aquaporin-4 deletion in mice reduces brain edema after acute water intoxication and ischemic stroke. Nat Med 6: 159-163.
29. Furman CS, Gorelick-Feldman DA, Davidson KG, Yasumura T, Neely JD, et al. (2003) Aquaporin-4 square array assembly: opposing actions of M1 and M23 isoforms. Proc Natl Acad Sci U S A 100: 13609-13614.
30. Strand L, Moe SE, Solbu TT, Vaadal M, Holen T (2009) Roles of aquaporin-4 isoforms and amino acids in square array assembly. Biochemistry 48: 5785-5793.
31. Harries WE, Akhavan D, Miercke LJ, Khademi S, Stroud RM (2004) The channel architecture of aquaporin 0 at a 2.2-A resolution. Proc Natl Acad Sci U S A 101: 14045-14050.
32. Horsefield R, Norden K, Fellert M, Backmark A, Tornroth-Horsefield S, et al. (2008) High-resolution x-ray structure of human aquaporin 5. Proc Natl Acad Sci U S A 105: 13327-13332.
33. Fu D, Libson A, Miercke LJ, Weitzman C, Nollert P, et al. (2000) Structure of a glycerol-conducting channel and the basis for its selectivity. Science 290: 481-486.
34. Ho JD, Yeh R, Sandstrom A, Chorny I, Harries WE, et al. (2009) Crystal structure of human aquaporin 4 at 1.8 A and its mechanism of conductance. Proc Natl Acad Sci U S A 106: 7437-7442.
35. Yang B, van Hoek AN, Verkman AS (1997) Very high single channel water permeability of aquaporin-4 in baculovirus-infected insect cells and liposomes reconstituted with purified aquaporin-4. Biochemistry 36: 7625-7632.
36. Kozono D, Ding X, Iwasaki I, Meng X, Kamagata Y, et al. (2003) Functional expression and characterization of an archaeal aquaporin. AqpM from methanothermobacter marburgensis. J Biol Chem 278: 10649-10656.
37. Werten PJ, Hasler L, Koenderink JB, Klaassen CH, de Grip WJ, et al. (2001) Large-scale purification of functional recombinant human aquaporin-2. FEBS Lett 504: 200-205.
38. Daniels BV, Jiang JS, Fu D (2004) Crystallization and preliminary crystallographic analysis of the Escherichia coli water channel AqpZ. Acta Crystallogr D Biol Crystallogr 60: 561-563.
39. Karlsson M, Fotiadis D, Sjovall S, Johansson I, Hedfalk K, et al. (2003) Reconstitution of water channel function of an aquaporin overexpressed and purified from Pichia pastoris. FEBS Lett 537: 68-72.
40. Lian J, Ding S, Cai J, Zhang D, Xu Z, et al. (2009) Improving aquaporin Z expression in Escherichia coli by fusion partners and subsequent condition optimization. Appl Microbiol Biotechnol 82: 463-470.
41. Hovijitra NT, Wuu JJ, Peaker B, Swartz JR (2009) Cell-free synthesis of functional aquaporin Z in synthetic liposomes. Biotechnol Bioeng 104: 40-49.
42. Silberstein C, Bouley R, Huang Y, Fang P, Pastor-Soler N, et al. (2004) Membrane organization and function of M1 and M23 isoforms of aquaporin-4 in epithelial cells. Am J Physiol Renal Physiol 287: F501-511.
43. Yukutake Y, Hirano Y, Suematsu M, Yasui M (2009) Rapid and reversible inhibition of aquaporin-4 by zinc. Biochemistry 48: 12059-12061.
44. Splinter PL, Masyuk AI, Marinelli RA, LaRusso NF (2004) AQP4 transfected into mouse cholangiocytes promotes water transport in biliary epithelia. Hepatology 39: 109-116.
45. Schneider B, Junge F, Shirokov VA, Durst F, Schwarz D, et al. Membrane protein expression in cell-free systems. Methods Mol Biol 601: 165-186.