A bacterial cell-free expression system to produce membrane proteins and proteoliposomes: From cDNA to functional assay

Current Protocols in Protein Science

Volumn , Issue SUPPL.54, 2008, Pages

  Liguori, Lavinia ^a   Marques, Bruno ^a   Lenormand, Jean Luc ^a  

^a LABORATOIRE TIMC IMAG   (France)

Author keywords

Cell free expression system; Delivery system; Membrane proteins production; One step proteoliposomes synthesis

Indexed keywords

BACTERIUM LYSATE; BUFFER; COMPLEMENTARY DNA; CYTOCHROME C; MEMBRANE PROTEIN; REAGENT; PROTEOLIPOSOME;

AGAR GEL ELECTROPHORESIS; ASSAY; BACTERIUM TRANSFORMATION; CELL FREE SYSTEM; CHLOROPLAST; CLONING VECTOR; CONTROLLED STUDY; ENZYME RELEASE; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EXPRESSION SYSTEM; IMMUNOBLOTTING; LIPOSOMAL GENE DELIVERY SYSTEM; METHODOLOGY; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; PROTEOLIPOSOME; RAPID TRANSLATION SYSTEM; REVIEW; SPINACH; STAINING; THYLAKOID MEMBRANE; ANTIBODY DETECTION; ARTICLE; BACTERIAL CELL FREE EXPRESSION SYSTEM; CELL MEMBRANE PERMEABILITY; CLINICAL PROTOCOL; COLORIMETRY; DNA FRAGMENTATION; ENDOSPERM; HYDROPHOBICITY; IMMUNOLOCALIZATION; PROCESS OPTIMIZATION; PROMOTER REGION; PROTEIN FOLDING; PROTEIN FUNCTION; TRANSMISSION ELECTRON MICROSCOPY; WESTERN BLOTTING;

BACTERIA (MICROORGANISMS); EUKARYOTA;

EID: 71849093652     PISSN: 19343655     EISSN: 19343663     Source Type: Journal    
DOI: 10.1002/0471140864.ps0522s54     Document Type: Review

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