Overexpression of the pineapple fruit bromelain gene (BAA) in transgenic Chinese cabbage (Brassica rapa) results in enhanced resistance to bacterial soft rot

Electronic Journal of Biotechnology

Volumn 11, Issue 1, 2008, Pages

  Jung, Yu Jin ^a   Choi, Chang Sun ^a   Park, Jin Heui ^a   Kang, Hee Wan ^a   Choi, Je Eul ^b   Nou, Ill Sup ^c   Soon, Youl Lee ^a   Kang, Kwon Kyoo ^a  

^a Hankyong National University   (South Korea)

^b Chungnam National University   (South Korea)

^c Sunchon National University   (South Korea)

Author keywords

Brassica rapa; Fruit bromelain; Soft rot resistance; Transgenic plant

Indexed keywords

CLONING; FRUITS; GENE ENCODING; GENE EXPRESSION; GENES; PATHOGENS; SPOILAGE; VIRUSES;

BRASSICA RAPA; CAULIFLOWER MOSAIC VIRUS; FRUIT BROMELAIN; PECTOBACTERIUM CAROTOVORUM; PLANT EXPRESSION VECTORS; SOFT-ROT; THERAPEUTIC ACTIVITY; TRANSGENIC PLANTS;

BROMELAIN;

ANTIBIOTIC RESISTANCE; ARTICLE; CAULIFLOWER MOSAIC VIRUS; CONTROLLED STUDY; GENE OVEREXPRESSION; NONHUMAN; PROMOTER REGION; TRANSGENIC PLANT; TURNIP;

ANANAS COMOSUS; BACTERIA (MICROORGANISMS); BRASSICA RAPA; BRASSICA RAPA SUBSP. PEKINENSIS; CAULIFLOWER MOSAIC VIRUS; PECTOBACTERIUM CAROTOVORUM;

EID: 47149089505     PISSN: None     EISSN: 07173458     Source Type: Journal    
DOI: 10.2225/vol11-issue1-fulltext-5     Document Type: Article

References (35)

1. BEERS, Eric P.; WOFFENDEN, Bonnie J. and ZHAO, Chengsong. Plant proteolytic enzymes: possible roles during programmed cell death. Plant Molecular Biology, October 2000, vol. 44, no. 3, p. 399-415.
2. BEYENE, Getu; FOYER, Christine H. and KUNERT, Karl J. Two new cysteine proteinases with specific expression patterns in mature and senescent tobacco (Nicotiana tabacum L.) leaves. Journal of Experimental Botany, March 2006, vol. 57, no. 6, p. 1431-1443.
3. BROEKAERT, W.F.; CAMMUE, B.P.A.; DE BOLLE, M.F.C.; THEVISSEN, K.; DE SAMBLANX, G.W. and OSBORN, R.W. Antimicrobial peptides from plants. Critical Reviews in Plant Sciences, 1997, vol. 16, no. 3, p. 297-323.
4. DIXON, R.A. The phytoalexin response: elicitation, signaling and control of host gene expression. Biological Reviews of the Cambridge Philosophical Society, 1986, vol. 61, p. 239-291.
5. FRAY, Rupert G.; THROUP, John P.; DAYKIN, Mavis; WALLACE, Andrew; WILLIAMS, Paul; STEWART, Gordon S.A.B. and GRIERSON, Donald. Plants genetically modified to produce N-acyl-homoserine lactones communicate with bacteria. Nature Biotechnology, October 1999, vol. 17, no. 10, p. 1017-1020.
6. GER, Mang-Jye; CHEN, Cheng-Hsien; HWANG, Shaw-Yhi; HUANG, Hsiang-En; PODILE, Appa Rao; DAYAKAR, Bradi Venkata and FENG, Teng-Yung. Constitutive expression of hrap gene in transgenic tobacco plant enhances resistance against virulent bacterial pathogens by induction of a hypersensitive response. Molecular Plant-Microbe Interactions, August 2002, vol. 15, no. 8, p. 764-773.
7. HELIAS, V.; ANDRIVON, D. and JOUAN, B. Development of symptoms caused by Erwinia carotovora ssp. atroseptica under field conditions and their effects on the yield of individual potato plants. Plant Pathology, February 2000, vol. 49, no. 1, p. 23-32.
8. KIKUMOTO, T. Studies on soft rot disease of Chinese cabbage in Japan. In: TALAKAR, T.S. and GRIGGS, T.D. eds. Chinese Cabbage. Proceedings of the First International Symposium AVRDC. Shanhua, Tainan, Taiwan, 1981, p. 113-127.
9. LAM, Eric. Vacuolar proteases livening up programmed cell death. Trends in Cell Biology, March 2005, vol. 15, no. 3, p. 124-127.
10. LI, C.W. The origin, evolution, taxonomy and hybridization of Chinese cabbage. In: TALAKAR, T.S. and GRIGGS, T.D. eds. Chinese cabbage. Proceedings of the First International Symposium AVRDC. Shanhua, Tainan, Taiwan, 1981, p. 3-10.
11. LINKE, Christoph; CONRATH, Uwe; JEBLICK, Wolfgang; BETSCHE, Thomas; MAHN, Andreas; DURING, Klaus and NEUHAUS, H. Ekkehard. Inhibition of the plastidic ATP/ADP transporter protein primes potato tubers for augmented elicitation of defense responses and enhances their resistance against Erwinia carotovora. Plant Physiology, August 2002, vol. 129, no. 4, p. 1607-1615.
12. LINTHORST, H.J.M. Pathogenesis-related proteins of plants. Critical Reviews in Plant Sciences, 1991, vol. 10, p. 123-150.
13. LUERTI, M. and VIGNALI, M.L. Influence of bromelain on penetration of antibiotics in uterus, salpinx and ovary. Drugs under Experimental and Clinical Research, 1978, vol. 4, no. 1, p. 45-48.
14. MAE, Andres; MONTESANO, Marcos; KOIV, Viia and PALVA, E. Tapio. Transgenic plants producing the bacterial pheromone N-Acyl-Homoserine lactone exhibit enhanced resistance to the bacterial phytopathogen Erwinia carotovora. Molecular Plant-Microbe Interactions, September 2001, vol. 14, no. 9, p. 1035-1042.
15. MAURER, H.R. Bromelain: biochemistry, pharmacology and medical use. Cellular and Molecular Life Sciences, August 2001, vol. 58, no. 9, p. 1234-1245.
16. MURASHIGE, Toshio and SKOOG, Folke. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, July 1962, vol. 15, no. 3, p. 473-497.
17. MUTA, E.; ARAMAKI, H.; TAKATAY; KONO, A.; OKAMOTO, Y. and OTA, S. Cloning and sequencing of fruit bromelain [online]. January 1993, D14059. Available from Internet: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id= 2342495.
18. MYNOTT, Tracey L.; CROSSETT, Ben and PRATHALINGAM, S. Radhika. Proteolytic inhibition of Salmonella enterica serovar typhimurium-induced activation of the mitogen-activated protein kinases ERK and JNK in cultured human intestinal cells. Infection and Immunity, January 2002, vol. 70, no. 1, p. 86-95.
19. ORSINI, Roger A. Bromelain. Plastic and Reconstructive Surgery, December 2006, vol. 118, no. 7, p. 1640-1644.
20. PANDEY, Ajay-Kumar; GER, Mang-Jye; HUANG, Hsiang-En; YIP, Mei-Kuen; ZENG, Jiqing and FENG, Teng-Yung. Expression of the hypersensitive response-assisting protein in Arabidopsis results in hairpin-dependent hypersensitive cell death in response to Erwinia carotovora. Plant Molecular Biology, November 2005, vol. 59, no. 5, p. 771-780.
21. SHAFER, W.M.; KATZIF, S.; BOWERS, S.; FALLON, M.; HUBALEK, M.; REED, M.S.; VEPREK, P. and POHL, J. Tailoring an antibacterial peptide of human lysosomal cathepsin G to enhance its broad-spectrum action against antibiotic-resistant bacterial pathogens. Current Pharmaceutical Design, April 2002, vol. 8, no. 9, p. 695-702.
22. SHEN, Songhai; LI, Qingshun; HE, Sheng-Yang; BARKER, Kenneth R.; LI, Debao and HUNT, Arthur G. Conversion of compatible plant-pathogen interactions into incompatible interactions by expression of the Pseudomonas syringae pv. syringae 61 hrmA gene in transgenic tobacco plants. Plant Journal, July 2000, vol. 23, no. 2, p. 205-213.
23. 2+-dependent cysteine protease is associated with anoxia-induced root tip death in maize. Journal of Experimental Botany, April 2000, vol. 51, no. 345, p. 721-730.
24. TANI, Kenji; OGUSHI, Fumitaka; SHIMIZU, Teruki and SONE, Saburo. Protease-induced leukocyte chemotaxis and activation: roles in host defense and inflammation. The Journal of Medical Investigation, August 2001, vol. 48, no. 3-4, p. 133-141.
25. TAUSSIG, Steven J. and BATKIN, Stanley. Bromelain, the enzyme complex of Pineapple (Ananas comusus) and its clinical application. An update. Journal of Ethnopharmacology, February-March 1988, vol. 22, no. 2, p. 191-203.
26. TINOZZI, S. and VENEGONI, A. Effect of bromelain on serum and tissue levels of amoxycillin. Drugs under Experimental and Clinical Research, 1978, vol. 4, p. 39-44.
27. TOTH, I.K.; NEWTON, J.A.; HYMAN, L.J.; LEES, A.K.; DAYKIN, M.; ORTORI, C.; WILLIAMS, P. and FRAY, R.G. Potato plants genetically modified to produce N-acylhomoserine lactones increase susceptibility to soft rot Erwiniae. Molecular Plant-Microbe Interactions, August 2004, vol. 17, no. 8, p. 880-887.
28. VAILLEAU, Fabienne; DANIEL, Xavier; TRONCHET, Maurice; MONTILLET, Jean-Luc; TRIANTAPHYLIDES, Christian and ROBY, Dominique. A R2R3-MYB gene, AtMYB30, acts as a positive regulator of the hypersensitive cell death program in plants in response to pathogen attack. Proceedings of the National Academy of Sciences of the United States of America, July 2002, vol. 99, no. 15, p. 10179-10184.
29. VAN LOON, L.C. and VAN KAMMEN, A. Polyacrylamide disc electrophoresis of the soluble leaf proteins from Nicotiana tabacum var. 'Samsun' and 'Samsun NN'. II. Changes in protein constitution after infection with TMV. Virology, February 1970, vol. 40, no. 2, p. 199-211.
30. VERBERNE, Marianne C., VERPOORTE, Rob; BOL, John F.; MERCADO-BLANCO, Jesus and LINTHORST, Huub J.M. Overproduction of salicylic acid in plants by bacterial transgenes enhances pathogen resistance. Nature Biotechnology, July 2000, vol. 18, no. 7, p. 779-783.
31. YAMAGISHI, Hiroshi; YOSHIKAWA, Hiroaki and YUI, Susumu. Leaf morphology and soft rot resistance in offspring of a somatic hybrid between Chinese cabbage and kale (Cruciferae). Euphytica, June 1990, vol. 47, no. 3, p. 215-221.
32. YIP, Mei-Kuen; HUANG, Hsiang-En; GER, Mang-Jye; CHIU, Shih-Hua; TSAI, Yuh-Chih; LIN, Chin-I and FENG, Teng-Yung. Production of soft rot resistant calla lily by expressing a ferredoxin-like protein gene (pflp) in transgenic plants. Plant Cell Reports, April 2007, vol. 26, no. 4, p. 449-457.
33. YOSHIKAWA, H.; SAWADA, T.; YAMAGISHI, H. and YUI, S. Soft rot resistance of progenies of somatic hybrid: Chinese cabbage plus Kale. Journal of Japan Society of Horticultural Science, 1989, vol. 58, p. 190-191.
34. YUN, D.-J.; BRESSAN, R.A. and HASEGAWA, P.M. Plant antifungal proteins. In: JANICK, J. ed. Plant Breeding Reviews. John Wiley, New York, 1997, vol. 14, p. 39-88.
35. ZHOU, Jianmin; LOH, Ying-Tsu; BRESSAN, Ray A. and MARTIN, Gregory B. The tomato gene Pti1 encodes a serine/threonine kinase that is phosphorylated by Pto and is involved in the hypersensitive response. Cell, December 1995, vol. 83, no. 6, p. 925-935.