Cloning, expression, and characterization of a novel (s)-specific alcohol dehydrogenase from lactobacillus kefir

Applied Biochemistry and Biotechnology

Volumn 160, Issue 1, 2010, Pages 19-29

  Chen, Qilei ^a   Hu, Youjia ^a,b   Zhao, Wenjie ^a   Zhu, Chunbao ^a   Zhu, Baoquan ^a  

^a SHANGHAI INSTITUTE OF PHARMACEUTICAL INDUSTRY   (China)

^b NONE   (China)

Author keywords

(S) specific alcohol dehydrogenase; Lactobacillus kefir; NADH dependent; TAIL PCR

Indexed keywords

ACETOPHENONES; ALCOHOL DEHYDROGENASE; AMINO ACID SEQUENCE; COFACTORS; E. COLI; GENBANK; GENBANK DATABASE; GENOMIC DNA; HIGH ENANTIOSELECTIVITY; MEDIUM-CHAIN ALCOHOLS; NUCLEOTIDE SEQUENCES; OPEN READING FRAME; PHENYLETHANOL; PURIFIED ENZYME; SIMILARITY SEARCH; SUBSTRATE SPECIFICITY;

AMINO ACIDS; CLONING; ENANTIOSELECTIVITY; ESCHERICHIA COLI; ETHANOL; GENE ENCODING; KETONES; ORGANIC ACIDS; ZINC;

POLYMERASE CHAIN REACTION;

ACETOPHENONE; ALCOHOL DEHYDROGENASE; GENOMIC DNA; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL STRAIN; CLONING; DNA EXTRACTION; ENANTIOSELECTIVITY; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ESCHERICHIA COLI; GAS CHROMATOGRAPHY; GENE EXPRESSION; GENETIC ANALYSIS; LACTOBACILLUS; LACTOBACILLUS KEFIR; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLASMID; POLYMERASE CHAIN REACTION;

ALCOHOL DEHYDROGENASE; AMINO ACID SEQUENCE; CLONING, MOLECULAR; COENZYMES; ESCHERICHIA COLI; GENE EXPRESSION; LACTOBACILLUS; MOLECULAR SEQUENCE DATA; NAD; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

LACTOBACILLUS KEFIRI;

EID: 73649139993     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12010-008-8442-6     Document Type: Article

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