Expression, purification, and characterization of a recombinant neutral ceramidase from mycobacterium tuberculosis

Bioscience, Biotechnology and Biochemistry

Volumn 74, Issue 2, 2010, Pages 316-321

  Okino, Nozomu ^a   Ikeda, Rie ^a   Ito, Makoto ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Ceramidase; Ceramide; Mycobacterium tuberculosis; Pseudomonas aeruginosa; Sphingosine

Indexed keywords

CERAMIDES; DODECANOIC ACID; ERYTHRO; GEL-FILTRATION; IN-VITRO; MICHAELIS-MENTEN KINETIC; MOLECULAR CLONING; MYCOBACTERIUM TUBERCULOSIS; PH OPTIMA; PSEUDOMONAS AERUGINOSA; PURIFIED ENZYME; RECOMBINANT ENZYMES; SATURATED FATTY ACID; SDS-PAGE; SINGLE BAND; SPHINGOSINE; UNSATURATED FATTY ACIDS;

ACIDS; AMIDES; CLONING; ENZYMES; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENE EXPRESSION; HYDROLYSIS; PURIFICATION; REACTION KINETICS; SYNTHESIS (CHEMICAL);

FATTY ACIDS;

7 NITROBENZ 2 OXA 1,3 DIAZOL 4 YL; 7-NITROBENZ-2-OXA-1,3-DIAZOL-4-YL; CERAMIDE; FATTY ACID; LAURIC ACID; LAURIC ACID DERIVATIVE; MONOUNSATURATED FATTY ACID; NEUTRAL CERAMIDASE; NITROBENZENE DERIVATIVE; PYRROLE DERIVATIVE; RECOMBINANT PROTEIN; SPHINGOSINE; UNSATURATED FATTY ACID;

ARTICLE; CATALYSIS; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; HYDROLYSIS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MYCOBACTERIUM TUBERCULOSIS; PH;

AZOLES; CATALYSIS; CERAMIDES; ESCHERICHIA COLI; FATTY ACIDS; FATTY ACIDS, MONOUNSATURATED; FATTY ACIDS, UNSATURATED; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; LAURIC ACIDS; MYCOBACTERIUM TUBERCULOSIS; NEUTRAL CERAMIDASE; NITROBENZENES; RECOMBINANT PROTEINS; SPHINGOSINE; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM TUBERCULOSIS; PSEUDOMONAS AERUGINOSA;

EID: 77249137735     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.90645     Document Type: Article

References (29)

1. Gatt S, J. Biol. Chem., 238, 3131-3133 (1963).
2. Kita K, Okino N, and Ito M, Biochim. Biophys. Acta, 1485, 111-120 (2000).
3. Okino N, He X, Gatt S, Sandhoff K, Ito M, and Schuchman EH, J. Biol. Chem., 278, 29948-29953 (2003).
4. Ito M, Okino N, Tani M, Mitsutake S, and Kita K, ''Molecular Evolution of Neutral Ceramidase: From Bacteria to Mammals,'' Landes Bioscience, Austin, pp. 41-48 (2002).
5. Okino N, Tani M, Imayama S, and Ito M, J. Biol. Chem., 273, 14368-14373 (1998).
6. Okino N, Ichinose S, Omori A, Imayama S, Nakamura T, and Ito M, J. Biol. Chem., 274, 36616-36622 (1999).
7. Monjusho H, Okino N, Tani M, Maeda M, Yoshida M, and Ito M, Biochem. J., 376, 473-479 (2003).
8. Pata MO, Wu BX, Bielawski J, Xiong TC, Hannun YA, and Ng CK, Plant J., 55, 1000-1009 (2008).
9. Yoshimura Y, Okino N, Tani M, and Ito M, J. Biochem. (Tokyo), 132, 229-236 (2002).
10. Yoshimura Y, Tani M, Okino N, Iida H, and Ito M, J. Biol. Chem., 279, 44012-44022 (2004).
11. Mitsutake S, Tani M, Okino N, Mori K, Ichinose S, Omori A, Iida H, Nakamura T, and Ito M, J. Biol. Chem., 276, 26249-26259 (2001).
12. Tani M, Okino N, Mori K, Tanigawa T, Izu H, and Ito M, J. Biol. Chem., 275, 11229-11234 (2000).
13. El Bawab S, Roddy P, Qian T, Bielawska A, Lemasters JJ, and Hannun YA, J. Biol. Chem., 275, 21508-21513 (2000).
14. Hwang YH, Tani M, Nakagawa T, Okino N, and Ito M, Biochem. Biophys. Res. Commun., 331, 37-42 (2005).
15. Acharya U, Patel S, Koundakjian E, Nagashima K, Han X, and Acharya JK, Science, 299, 1740-1743 (2003).
16. Rohrbough J, Rushton E, Palanker L, Woodruff E, Matthies HJ, Acharya U, Acharya JK, and Broadie K, J. Neurosci., 24, 7789-7803 (2004).
17. Kono M, Dreier JL, Ellis JM, Allende ML, Kalkofen DN, Sanders KM, Bielawski J, Bielawska A, Hannun YA, and Proia RL, J. Biol. Chem., 281, 7324-7331 (2006).
18. Okino N and Ito M, J. Biol. Chem., 282, 6021-6030 (2007).
19. Inoue T, Okino N, Kakuta Y, Hijikata A, Okano H, Goda HM, Tani M, Sueyoshi N, Kambayashi K, Matsumura H, Kai Y, and Ito M, J. Biol. Chem., 284, 9566-9577 (2009).
20. Tani M, Kita K, Komori H, Nakagawa T, and Ito M, Anal. Biochem., 263, 183-188 (1998).
21. Qing G, Ma LC, Khorchid A, Swapna GV, Mal TK, Takayama MM, Xia B, Phadtare S, Ke H, Acton T, Montelione GT, Ikura M, and Inouye M, Nat. Biotechnol., 22, 877-882 (2004).
22. Laemmli UK, Nature, 227, 680-685 (1970).
23. Merrill AH Jr, Wang E, Mullins RE, Jamison WC, Nimkar S, and Liotta DC, Anal. Biochem., 171, 373-381 (1988).
24. Lichtenberg D, Robson RJ, and Dennis EA, Biochim. Biophys. Acta, 737, 285-304 (1983).
25. Tani M, Okino N, Mitsutake S, Tanigawa T, Izu H, and Ito M, J. Biol. Chem., 275, 3462-3468 (2000).
26. Tani M, Okino N, Mitsutake S, and Ito M, J. Biochem. (Tokyo), 125, 746-749 (1999).
27. Petrache I, Natarajan V, Zhen L, Medler TR, Richter AT, Cho C, Hubbard WC, Berdyshev EV, and Tuder RM, Nat. Med., 11, 491-498 (2005).
28. Cole ST, Brosch R, Parkhill J, Garnier T, Churcher C, Harris D, Gordon SV, Eiglmeier K, Gas S, Barry CE3rd, Tekaia F, Badcock K, Basham D, Brown D, Chillingworth T, Connor R, Davies R, Devlin K, Feltwell T, Gentles S, Hamlin N, Holroyd S, Hornsby T, Jagels K, Krogh A, McLean J, Moule S, Murphy L, Oliver K, Osborne J, Quail MA, Rajandream MA, Rogers J, Rutter S, Seeger K, Skelton J, Squares R, Squares S, Sulston JE, Taylor K, Whitehead S, and Barrell BG, Nature, 393, 537-544 (1998).
29. Raynaud C, Guilhot C, Rauzier J, Bordat Y, Pelicic V, Manganelli R, Smith I, Gicquel B, and Jackson M, Mol. Microbiol., 45, 203-217 (2002).