Human Dickkopf-1 (huDKK1) protein: Characterization of glycosylation and determination of disulfide linkages in the two cysteine-rich domains

Protein Science

Volumn 20, Issue 11, 2011, Pages 1802-1813

  Haniu, Mitsuru ^a   Horan, Tom ^a   Spahr, Chris ^a   Hui, John ^a   Fan, Wei ^a   Chen, Ching ^a   Richards, William G ^a   Lu, Hsieng S ^a  

^a AMGEN INC   (United States)

Author keywords

Cysteine rich domains; Disulfide mapping; Human DKK1; N O linked glycans

Indexed keywords

DICKKOPF 1 PROTEIN; DISULFIDE; ENDOPROTEINASE; GLYCAN; LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN 6; N ETHYLMALEIMIDE; OLIGOSACCHARIDE; PROTEINASE; SIALIC ACID; THERMOLYSIN; TRIS(2 CARBOXYETHYL)PHOSPHINE; TRYPSIN; UNCLASSIFIED DRUG;

ALKYLATION; ANIMAL CELL; ARTICLE; CARBOHYDRATE ANALYSIS; CHO CELL; CYSTEINE RICH DOMAIN; DISULFIDE BOND; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; PEPTIDE MAPPING; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN ISOLATION; PROTEIN MOTIF; PROTEIN PURIFICATION; PROTEIN STRUCTURE; RECEPTOR BINDING;

AMINO ACID SEQUENCE; ANIMALS; CARBOHYDRATE CONFORMATION; CARBOHYDRATE SEQUENCE; CELL LINE; CHO CELLS; CRICETINAE; CYSTEINE; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOSYLATION; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; MOLECULAR SEQUENCE DATA; PEPTIDE MAPPING; POLYSACCHARIDES; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SEQUENCE ANALYSIS, PROTEIN; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; WNT SIGNALING PATHWAY;

CRICETULUS GRISEUS;

EID: 80054761995     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1002/pro.705     Document Type: Article

References (31)

1. Semenov MV, Tamai K, Brott BK, Kuhl M, Soko S, He X (2001) Head inducer Dokkopf-1 is a ligand for Wnt coreceptor LRP6. Curr Biol 11: 951-961. (Pubitemid 32589186)
2. Li X, Liu P, Liu W, Maye P, Zhang J, Zhang Y, Hurley M, Guo C, Boskey A, Sun L, Harris SE, Rowe DW, Ke HZ, Wu D (2005) Dkk2 has a role in terminal osteoblast differentiation and mineralized matrix formation. Nat Genet 37: 945-952. (Pubitemid 43086150)
3. Glinka A, Wu W, Delius H, Monaghan AP, Blumenstock C, Niehrs C (1998) Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction. Nature 391: 357-362. (Pubitemid 28093502)
4. Semenov MV, He X (2006) LRP5 mutations linked to high bone mass disease cause reduced LRP5 binding and inhibition by SOST. J Biol Chem 281:38276-38284. (Pubitemid 46043307)
5. Cadigan KM, Nusse R (1997) Wnt signaling: a common theme in animal development. Genes Dev 11:3286-3305. (Pubitemid 28023954)
6. Westendorf JJ, Kahler RA, Schroeder TM (2004) Wnt signaling in osteoblasts and bone diseases. Gene 341:19-39. (Pubitemid 39335880)
7. Krishnan V, Bryant HU, MacDougald OA (2006) Regulation of bone mass by Wnt signaling. J Clin Invest 116: 1202-1209.
8. Li X, Zhang Y, Kang H, Liu W, Liu P, Zhang J, Harris SE, Wu D (2005) Scleorostin binds to LRP5/6 and antagonizes canonical Wnt signaling. J Biol Chem 280:19883-19887. (Pubitemid 41379516)
9. Bourhis E, Tam C, Franke Y, Bazan JF, Ernst J, Hwang J, Costa M, Cochran AG, Hannoush RN (2010) Reconstitution of Frizzled8-Wnt3a-LRP6 signaling complex reveals multiple Wnt and DKK1 binding sites on LRP6. J Biol Chem 285: 9172-9179.
10. Fedi P, Bafico A, Soria AN, Burgess WH, Miki T, Bottaro DP, Kraus MH, Aaronson SA (1999) Isolation and biochemical characterization of the human DKK1 homologue, a novel inhibitor of mammalian Wnt signaling. J Biol Chem 274: 19465-19472.
11. Mao B, Wu W, Li Y, Hoppe D, Stannek P, Glinka A, Niehrs C (2001) LDL receptor-related protein 6 is a receptor for Dkk proteins. Nature 411: 321-325. (Pubitemid 32467039)
12. Mukhopadhyay M, Shtrom S, Rodriguez-Esteban C, Chen L, Tsukui T, Gomer L, Dorward DW, Glinka A, Grinberg A, Huang SP, Niehrs C, Belmonte JCI, Westphal H (2001) Dickkopf1 is required for embryonic head induction and limb morphogenesis in the mouse. Dev Cell 1: 423-434. (Pubitemid 33586091)
13. Morvan F, Boulukos K, Clement-Lacroix P, Roman SR, Suc-Royer I, Vayssiere B, Ammann P, Martin P, Pinho S, Pognonec P, Mollat P, Niehrs C, Baron R, Rawadi G (2006) Deletion of a single allele of the DKK1 gene leads to an increase in bone formation and bone mass. J Bone Mineral Res 21: 934-945. (Pubitemid 43788000)
14. Li J, Sarosi I, Cattley RC, Pretorius J, Asuncion F, Grisanti M, Morony S, Adamu S, Geng ZP, Qiu WR, Kosetnuik P, Lacy DL, Simonet S, Bolon B, Qian X, Shalhoub V, Ominsky MS, Ke HZ, Li X, Richards WG (2006) DKK1-mediated inhibition of Wnt signaling in bone results in osteopenia. Bone 39: 754-766. (Pubitemid 44311706)
15. Diarra D, Stolina M, Polzer K, Zwerina J, Ominsky MS, Dwyer D, Korb A, Smolen J, Hoffmann M, Scheinecker C, der Heide D, Landewe R, Lacey D, Richards WG, Schett G (2007) Dickkopf-1 is a master regulator of joint remodeling. Nat Med 13: 156-163.
16. Tian E, Zhan FH, Walker R, Rasmussen E, Ma YP, Barlogie B, Shaughnessy JD (2003) The role of the Wnt-signaling antagonist DKK1 in the development of osteolytic lesions in multiple myeloma. New England J Med 349: 2483-2493.
17. Hoeppner LH, Secreto FJ, Westendorf J (2009) Wnt signaling as a therapeutic target for bone diseases. Exp Opin Ther Targets 13: 485-496.
18. MaCarthy HS, Marshall MJ (2010) Dickkopf-1 as a potential therapeutic target in Paget's disease of bone. Exp Opin Ther Targets 14: 221-230.
19. Kubota T, Michigami T (2009) Wnt signaling in bone metabolism. J Bone Miner Metab 27: 265-271.
20. Choi Y, Arron JR, Townsend MJ (2009) Promising bone-related therapeutic targets for rheumatoid arthritis. Nature Rev Rheumatol 5: 543-548.
21. Pinzone JJ, Hall BM, Thudi NK, Vonau M, Qiang Y-W, Rosol TJ, Shaughnessy JD (2009) The role of Dickkopf-1 in bone development, homeostasis, and disease. Blood 15: 517-525.
22. Krupnik VE, Sharp JD, Jiang C, Robison K, Chickering TW, Amaravadi L, Brown DE, Guyot D, Mays G, Leiby K, Chang B, Duong T, Goodearl ADJ, Gearing DP, Sokol SY, McCarthy SA (1999) Functional and structural diversity of the human Dickkopf gene family. Gene 238: 301-313. (Pubitemid 29447031)
23. Aravind L, Koonin EV (2000) A colipase fold in the carboxy-terminal domain of the Wnt antagonists -the Dickkopfs. Nature 8: R477-R478.
24. Fleury D, Gillard C, Lebhar H, Vayssiere B, Touitou R, Rawadi G, Mollat P (2008) Expression, purification and characterization of murine DKK1 protein. Protein Exp Purif 60: 74-81.
25. Brott BK, Sokol SY (2002) Regulation of Wnt/LRP signaling by distinct domains of Dickkopf proteins. Mol Cell Biol 22: 6100-6110. (Pubitemid 34857830)
26. Olivera BM, Rivier J, Clark C, Ramilo CA, Corpuz GP, Abogadie FC, Mena EE, Woodward SR, Hillyard DR, Cruz LJ (1990) Diversity of Conus neuropeptides. Science 249: 257-263.
27. Bures EJ, Hui JO, Young Y, Rohde Chow DT, Katta V, Rohde MF, Zeni L, Rosenfeld RD, Stark KL, Haniu M (1998) Determination of disulfide structure in Agouti related protein (AGRP) by stepwise reduction and alkylation. Biochemistry 37: 12172-12177. (Pubitemid 28411302)
28. Young Y, Zeni L, Rosenfeld RD, Stark K, Rohde MR, Haniu M (1999) Disulfide assignment of the C-terminal cysteine knot of Agouti-related protein (AGRP) by direct sequence analysis. J Peptide Res 54: 514-521.
29. Pruneau D, Angus JA (1990) [Omega]-Conotoxin GVIA, the N-type calcium channel inhibitor, is sympatholytic but not vagolytic: Consequences for hemodynamics and autonomic reflexes in conscious rabbits. J Cardio Pharmacol 16: 675-680. (Pubitemid 20305827)
30. Hubbard TJP, Murzin AG, Brenner SE, Chothia C (1997) SCOP: a structural classification of proteins database. Nucleic Acids Res 25: 236-239. (Pubitemid 27307008)
31. Frieda Reichsman F, Moore HM, Cumberledge S (1999) Sequence homology between Wingless/Wnt-1 and a lipid-binding domain in secreted phospholipase A2. Curr Biol 9: R353-R355. (Pubitemid 29257096)