메뉴 건너뛰기




Volumn 63, Issue 2, 1998, Pages 85-95

Measurement of volumetric (OUR) and determination of specific (qO2) oxygen uptake rates in animal cell cultures

Author keywords

Animal cell culture; Oxygen uptake rate; Oxygenation

Indexed keywords

ANIMAL CELL CULTURE; BIOREACTORS; METABOLISM; OXYGEN;

EID: 0344507414     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(98)00046-7     Document Type: Article
Times cited : (89)

References (60)
  • 1
    • 0026261912 scopus 로고
    • Biological responses of hybridoma cells to hydrodynamic shear in an agitated bioreactor
    • Abu-Reesh I., Kargi F. Biological responses of hybridoma cells to hydrodynamic shear in an agitated bioreactor. Enzyme Microb. Technol. 13:1991;913-919.
    • (1991) Enzyme Microb. Technol. , vol.13 , pp. 913-919
    • Abu-Reesh, I.1    Kargi, F.2
  • 4
    • 0029256482 scopus 로고
    • Determination of the respiration quotient in mammalian-cell culture in bicarbonate buffered media
    • Bonarius H.P.J., Degooijer C.D., Tramper J., Schmid G. Determination of the respiration quotient in mammalian-cell culture in bicarbonate buffered media. Biotechnol. Bioeng. 45:1995;524-535.
    • (1995) Biotechnol. Bioeng. , vol.45 , pp. 524-535
    • Bonarius, H.P.J.1    Degooijer, C.D.2    Tramper, J.3    Schmid, G.4
  • 5
    • 0020987672 scopus 로고
    • Growth and oxygen requirements of antibody producing mouse hybridoma cells in suspension culture
    • Copenhagen, Denmark. Karger, Basel
    • Boraston, R., Thompson, P.W., Garland, S., Birch, J.R., 1984. Growth and oxygen requirements of antibody producing mouse hybridoma cells in suspension culture. In: Proceedings of the 5th Meeting of ESACT, Copenhagen, Denmark. Karger, Basel, pp. 103-111.
    • (1984) In: Proceedings of the 5th Meeting of ESACT , pp. 103-111
    • Boraston, R.1    Thompson, P.W.2    Garland, S.3    Birch, J.R.4
  • 6
    • 0024595916 scopus 로고
    • Performance of an internal loop-lift bioreactor for the production of monoclonal antibodies by immobilized cells
    • Bugarski B., King G.A., Jovanovic G., Daugulis A.J., Goosen M.F.A. Performance of an internal loop-lift bioreactor for the production of monoclonal antibodies by immobilized cells. Appl. Microbiol. Biotechnol. 30:1989;264-269.
    • (1989) Appl. Microbiol. Biotechnol. , vol.30 , pp. 264-269
    • Bugarski, B.1    King, G.A.2    Jovanovic, G.3    Daugulis, A.J.4    Goosen, M.F.A.5
  • 7
    • 0027957574 scopus 로고
    • A method for simultaneous determination of solubility and transfer coefficient of oxygen in aqueous media using off-gas mass spectrometry
    • Dorresteijn R.C., Degooijer C.D., Tramper J., Beuvery E.C. A method for simultaneous determination of solubility and transfer coefficient of oxygen in aqueous media using off-gas mass spectrometry. Biotechnol. Bioeng. 43:1994;149-154.
    • (1994) Biotechnol. Bioeng. , vol.43 , pp. 149-154
    • Dorresteijn, R.C.1    Degooijer, C.D.2    Tramper, J.3    Beuvery, E.C.4
  • 9
    • 0030569899 scopus 로고    scopus 로고
    • On-line estimation of viable cells in a hybridoma culture at various DO levels using ATP balancing and redox potential measurement
    • Eyer K., Heinzle E. On-line estimation of viable cells in a hybridoma culture at various DO levels using ATP balancing and redox potential measurement. Biotechnol. Bioeng. 49:1996;227-283.
    • (1996) Biotechnol. Bioeng. , vol.49 , pp. 227-283
    • Eyer, K.1    Heinzle, E.2
  • 10
    • 0029635979 scopus 로고
    • Online gas-analysis in animal-cell cultivation. 2. Methods for oxygen-uptake rate estimation and its application to controlled feeding of glutamine
    • Eyer K., Oeggerli A., Heinzle E. Online gas-analysis in animal-cell cultivation. 2. Methods for oxygen-uptake rate estimation and its application to controlled feeding of glutamine. Biotechnol. Bioeng. 45:1995;54-62.
    • (1995) Biotechnol. Bioeng. , vol.45 , pp. 54-62
    • Eyer, K.1    Oeggerli, A.2    Heinzle, E.3
  • 12
    • 0021924992 scopus 로고
    • Oxygen uptake rate of mammallian cells in microcarrier culture - Response to changes in glucose concentration
    • Frame K.K., Hu W.-S. Oxygen uptake rate of mammallian cells in microcarrier culture - response to changes in glucose concentration. Biotechnol. Lett. 7:1985;147-152.
    • (1985) Biotechnol. Lett. , vol.7 , pp. 147-152
    • Frame, K.K.1    Hu, W.-S.2
  • 13
    • 0025412671 scopus 로고
    • Effects of stirring and sparging on cultured hybridoma cells
    • Gardner A.R., Gainer J.L., Kirwan D.J. Effects of stirring and sparging on cultured hybridoma cells. Biotechnol. Bioeng. 35:1990;940-947.
    • (1990) Biotechnol. Bioeng. , vol.35 , pp. 940-947
    • Gardner, A.R.1    Gainer, J.L.2    Kirwan, D.J.3
  • 14
    • 0026149467 scopus 로고
    • Measuring Kla with randomly pulsed dynamic method
    • Gauthier L., Thibault J., LeDuy A. Measuring Kla with randomly pulsed dynamic method. Biotechnol. Bioeng. 37:1991;889-893.
    • (1991) Biotechnol. Bioeng. , vol.37 , pp. 889-893
    • Gauthier, L.1    Thibault, J.2    Leduy, A.3
  • 15
    • 0022787791 scopus 로고
    • Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells
    • Glacken M.W., Fleischaker R.J., Sinskey A.J. Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells. Biotechnol. Bioeng. 28:1986;1376-1389.
    • (1986) Biotechnol. Bioeng. , vol.28 , pp. 1376-1389
    • Glacken, M.W.1    Fleischaker, R.J.2    Sinskey, A.J.3
  • 16
    • 0001556512 scopus 로고
    • High cell density culture of a hybridoma using perfluorocarbon to supply oxygen
    • Hamamoto K., Tokashiki M., Ichikawa Y., Murakami H. High cell density culture of a hybridoma using perfluorocarbon to supply oxygen. Agric. Biol. Chem. 51:1987;3415-3416.
    • (1987) Agric. Biol. Chem. , vol.51 , pp. 3415-3416
    • Hamamoto, K.1    Tokashiki, M.2    Ichikawa, Y.3    Murakami, H.4
  • 18
    • 33645573115 scopus 로고
    • Mass spectrometry for on-line monitoring of biotechnological processes
    • Heinzle E. Mass spectrometry for on-line monitoring of biotechnological processes. Adv. Biochem. Eng./Biotechnol. 35:1987;1-46.
    • (1987) Adv. Biochem. Eng./Biotechnol. , vol.35 , pp. 1-46
    • Heinzle, E.1
  • 19
    • 0026656092 scopus 로고
    • Present and potential applications of mass spectrometry for bioprocess research and control
    • Heinzle E. Present and potential applications of mass spectrometry for bioprocess research and control. J. Biotechnol. 25:1992;81-114.
    • (1992) J. Biotechnol. , vol.25 , pp. 81-114
    • Heinzle, E.1
  • 20
    • 0026417325 scopus 로고
    • A kinetic analysis of hybridoma growth and metabolism in continuous suspension culture on serum free medium
    • Hiller G.W., Aeschlimann A.D., Clark D.S., Blanch H.W. A kinetic analysis of hybridoma growth and metabolism in continuous suspension culture on serum free medium. Biotechnol. Bioeng. 38:1991;733-741.
    • (1991) Biotechnol. Bioeng. , vol.38 , pp. 733-741
    • Hiller, G.W.1    Aeschlimann, A.D.2    Clark, D.S.3    Blanch, H.W.4
  • 21
    • 0025700048 scopus 로고
    • Oxygen transfer rates in a mammalian cell culture bioreactor equipped with a cell-lift impeller
    • Johnson M., André G., Chavarie C., Archambault J. Oxygen transfer rates in a mammalian cell culture bioreactor equipped with a cell-lift impeller. Biotechnol. Bioeng. 35:1990;43-49.
    • (1990) Biotechnol. Bioeng. , vol.35 , pp. 43-49
    • Johnson, M.1    André, G.2    Chavarie, C.3    Archambault, J.4
  • 22
    • 0028393388 scopus 로고
    • Interactions between animal cells and gas bubbles: The influence of serum and pluronic F68 on the physical properties of the bubbles surface
    • Jordan M., Sucker H., Einsele A., Widmer F., Eppenberger H.M. Interactions between animal cells and gas bubbles: the influence of serum and pluronic F68 on the physical properties of the bubbles surface. Biotechnol. Bioeng. 43:1994;446-454.
    • (1994) Biotechnol. Bioeng. , vol.43 , pp. 446-454
    • Jordan, M.1    Sucker, H.2    Einsele, A.3    Widmer, F.4    Eppenberger, H.M.5
  • 23
    • 0025469222 scopus 로고
    • Damage mechanism of suspended animal cells in agitated bioreactors with and without bubble entrainment
    • Kunas K.T., Papoutsakis E.T. Damage mechanism of suspended animal cells in agitated bioreactors with and without bubble entrainment. Biotechnol. Bioeng. 36:1990;476-483.
    • (1990) Biotechnol. Bioeng. , vol.36 , pp. 476-483
    • Kunas, K.T.1    Papoutsakis, E.T.2
  • 24
    • 0028121775 scopus 로고
    • High density culture of mammalian cells with dynamic perfusion based on on-line oxygen uptake rate measurements
    • Kyung Y.-S., Peshwa M.V., Gryte D.M., Hu W.-S. High density culture of mammalian cells with dynamic perfusion based on on-line oxygen uptake rate measurements. Cytotechnology. 14:1994;183-190.
    • (1994) Cytotechnology , vol.14 , pp. 183-190
    • Kyung, Y.-S.1    Peshwa, M.V.2    Gryte, D.M.3    Hu, W.-S.4
  • 25
    • 0027626501 scopus 로고
    • Production of tPA in recombinant CHO cells under oxygen-limited conditions
    • Lin A.A., Kimura R., Miller W.M. Production of tPA in recombinant CHO cells under oxygen-limited conditions. Biotechnol. Bioeng. 42:1993;339-350.
    • (1993) Biotechnol. Bioeng. , vol.42 , pp. 339-350
    • Lin, A.A.1    Kimura, R.2    Miller, W.M.3
  • 26
    • 0025420089 scopus 로고
    • Comments on validity of dynamic measuring methods of oxygen diffusion coefficients in fermentation media with polarographic oxygen electrodes
    • Linek V., Sinkule J. Comments on validity of dynamic measuring methods of oxygen diffusion coefficients in fermentation media with polarographic oxygen electrodes. Biotechnol. Bioeng. 35:1990;1034-1041.
    • (1990) Biotechnol. Bioeng. , vol.35 , pp. 1034-1041
    • Linek, V.1    Sinkule, J.2
  • 27
    • 0019697726 scopus 로고
    • Chemical engineering use of catalysed sulfite oxidation kinetics for the determination of mass transfer characteristics of gas-liquid contactors
    • Linek V., Vacek V. Chemical engineering use of catalysed sulfite oxidation kinetics for the determination of mass transfer characteristics of gas-liquid contactors. Chem. Eng. Sci. 36:1981;1747-1768.
    • (1981) Chem. Eng. Sci. , vol.36 , pp. 1747-1768
    • Linek, V.1    Vacek, V.2
  • 28
    • 0015748587 scopus 로고
    • Measurement of aeration capacity of fermenters by rapidly responding oxygen probes
    • Linek V., Sobotka M., Prokop A. Measurement of aeration capacity of fermenters by rapidly responding oxygen probes. Biotechnol. Bioeng. Symp. 4:1973;429-453.
    • (1973) Biotechnol. Bioeng. Symp. , vol.4 , pp. 429-453
    • Linek, V.1    Sobotka, M.2    Prokop, A.3
  • 29
    • 0025430484 scopus 로고
    • On the first-order approximation to the response of dissolved oxygen electrodes for dynamic Kla estimation
    • Merchuk J.C., Yona S., Siegel M.H., Ben Zvi A. On the first-order approximation to the response of dissolved oxygen electrodes for dynamic Kla estimation. Biotechnol. Bioeng. 35:1990;1161-1163.
    • (1990) Biotechnol. Bioeng. , vol.35 , pp. 1161-1163
    • Merchuk, J.C.1    Yona, S.2    Siegel, M.H.3    Ben Zvi, A.4
  • 30
    • 0023483203 scopus 로고
    • Effects of dissolved oxygen concentration on hybridoma growth and metabolism in continuous culture
    • Miller W.M., Wilke C.R., Blanch H.W. Effects of dissolved oxygen concentration on hybridoma growth and metabolism in continuous culture. J. Cell. Physiol. 132:1987;524-530.
    • (1987) J. Cell. Physiol. , vol.132 , pp. 524-530
    • Miller, W.M.1    Wilke, C.R.2    Blanch, H.W.3
  • 31
    • 84988106827 scopus 로고
    • A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: Effect of nutrient concentration, dilution rate, and pH
    • Miller W.M., Blanch H.W., Wilke C.R. A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: effect of nutrient concentration, dilution rate, and pH. Biotechnol. Bioeng. 32:1988;947-965.
    • (1988) Biotechnol. Bioeng. , vol.32 , pp. 947-965
    • Miller, W.M.1    Blanch, H.W.2    Wilke, C.R.3
  • 32
    • 34250093455 scopus 로고
    • Transient response of hybridoma metabolism to changes in the oxygen supply rate in continuous culture
    • Miller W.M., Wilke C.R., Blanch H.W. Transient response of hybridoma metabolism to changes in the oxygen supply rate in continuous culture. Bioproc. Eng. 3:1988;103-111.
    • (1988) Bioproc. Eng. , vol.3 , pp. 103-111
    • Miller, W.M.1    Wilke, C.R.2    Blanch, H.W.3
  • 33
    • 0020998529 scopus 로고
    • Oxygen in human lymphoblastoid cell line cultures and effect of polymers in agitated and aerated cultures
    • Copenhagen, Denmark. Karger, Basel
    • Mizrahi, A., 1982. Oxygen in human lymphoblastoid cell line cultures and effect of polymers in agitated and aerated cultures. In: Proceedings of the 5th Meeting of the ESACT, Copenhagen, Denmark. Karger, Basel, pp. 93-102.
    • (1982) In: Proceedings of the 5th Meeting of the ESACT , pp. 93-102
    • Mizrahi, A.1
  • 34
    • 0026646385 scopus 로고
    • Enhancing bioprocess operability with generic software sensors
    • Montague G.A., Morris A.J., Tham M.T. Enhancing bioprocess operability with generic software sensors. J. Biotechnol. 25:1992;183-201.
    • (1992) J. Biotechnol. , vol.25 , pp. 183-201
    • Montague, G.A.1    Morris, A.J.2    Tham, M.T.3
  • 35
    • 0028979470 scopus 로고
    • Online gas-analysis in animal-cell cultivation. 1. Control of dissolved-oxygen and pH
    • Oeggerli A., Eyer K., Heinzle E. Online gas-analysis in animal-cell cultivation. 1. Control of dissolved-oxygen and pH. Biotechnol. Bioeng. 45:1995;42-53.
    • (1995) Biotechnol. Bioeng. , vol.45 , pp. 42-53
    • Oeggerli, A.1    Eyer, K.2    Heinzle, E.3
  • 36
    • 0027077620 scopus 로고
    • Effect of dissolved oxygen concentration on monoclonal antibody production in hybridoma cell cultures
    • Ogawa T., Kamihira M., Yoshida H., Iijima S., Kobayashi T. Effect of dissolved oxygen concentration on monoclonal antibody production in hybridoma cell cultures. J. Ferment Bioeng. 74:1992;372-378.
    • (1992) J. Ferment Bioeng. , vol.74 , pp. 372-378
    • Ogawa, T.1    Kamihira, M.2    Yoshida, H.3    Iijima, S.4    Kobayashi, T.5
  • 37
    • 0029977058 scopus 로고    scopus 로고
    • Interactive dual control of glucose and glutamine feeding in hybridoma cultivation
    • Oh G.S., Izuishi T., Inoue T., Hu W.S., Yoshida T. Interactive dual control of glucose and glutamine feeding in hybridoma cultivation. J. Ferment Bioeng. 81:1996;329-336.
    • (1996) J. Ferment Bioeng. , vol.81 , pp. 329-336
    • Oh, G.S.1    Izuishi, T.2    Inoue, T.3    Hu, W.S.4    Yoshida, T.5
  • 38
    • 0025514467 scopus 로고
    • Effects of dissolved oxygen on hybridoma cell growth, metabolism, and antibody production kinetics in continuous culture
    • Ozturk S.S., Palsson B.O. Effects of dissolved oxygen on hybridoma cell growth, metabolism, and antibody production kinetics in continuous culture. Biotechnol. Prog. 6:1990;437-446.
    • (1990) Biotechnol. Prog. , vol.6 , pp. 437-446
    • Ozturk, S.S.1    Palsson, B.O.2
  • 39
    • 0026254349 scopus 로고
    • Growth, metabolic, and antibody production kinetics of hybridoma cell culture: 2. Effects of serum concentration, dissolved oxygen concentration, and medium pH in a batch reactor
    • Ozturk S.S., Palsson B.O. Growth, metabolic, and antibody production kinetics of hybridoma cell culture: 2. Effects of serum concentration, dissolved oxygen concentration, and medium pH in a batch reactor. Biotechnol. Prog. 7:1991;481-494.
    • (1991) Biotechnol. Prog. , vol.7 , pp. 481-494
    • Ozturk, S.S.1    Palsson, B.O.2
  • 40
    • 0025878457 scopus 로고
    • Media additives for protecting freely suspended animal cells against agitation and aeration damage
    • Papoutsakis E.T. Media additives for protecting freely suspended animal cells against agitation and aeration damage. TIBTECH. 9:1991;316-324.
    • (1991) TIBTECH , vol.9 , pp. 316-324
    • Papoutsakis, E.T.1
  • 42
    • 0343864602 scopus 로고    scopus 로고
    • Monitoring and control of large scale vaccine production via dynamic oxygen uptake rate measurement
    • Villamoura, Portugal. Kluwer Academic, Dordrecht
    • Preissmann, A., Werner, R.G., Noé, W., 1997. Monitoring and control of large scale vaccine production via dynamic oxygen uptake rate measurement. In: Proceedings of the 14th Meeting of the ESACT, Villamoura, Portugal. Kluwer Academic, Dordrecht, pp. 169-174.
    • (1997) In: Proceedings of the 14th Meeting of the ESACT , pp. 169-174
    • Preissmann, A.1    Werner, R.G.2    Noé, W.3
  • 43
    • 0000748242 scopus 로고
    • Comparison of measured and calculated oxygen solubilities in fermentation media
    • Quicker G., Shumpe A., König B., Deckwer W.-D. Comparison of measured and calculated oxygen solubilities in fermentation media. Biotechnol. Bioeng. 23:1981;635-650.
    • (1981) Biotechnol. Bioeng. , vol.23 , pp. 635-650
    • Quicker, G.1    Shumpe, A.2    König, B.3    Deckwer, W.-D.4
  • 44
    • 0025499794 scopus 로고
    • Cell cycle and growth phase dependent variations in size distribution, antibody productivity, and oxygen demand in hybridoma cultures
    • Ramirez O.T., Mutharasan R. Cell cycle and growth phase dependent variations in size distribution, antibody productivity, and oxygen demand in hybridoma cultures. Biotechnol. Bioeng. 36:1990;839-848.
    • (1990) Biotechnol. Bioeng. , vol.36 , pp. 839-848
    • Ramirez, O.T.1    Mutharasan, R.2
  • 46
    • 0345141823 scopus 로고
    • On the methodology of dissolved oxygen saturation concentration measurement
    • Schneider H., Moser A. On the methodology of dissolved oxygen saturation concentration measurement. Biotechnol. Lett. 6:1984;295-300.
    • (1984) Biotechnol. Lett. , vol.6 , pp. 295-300
    • Schneider, H.1    Moser, A.2
  • 49
    • 0027909737 scopus 로고
    • Monoclonal-antibody productivity and the metabolic pattern of perfusion cultures under varying oxygen-tensions
    • Shi Y., Ryu D.D.Y., Park S.H. Monoclonal-antibody productivity and the metabolic pattern of perfusion cultures under varying oxygen-tensions. Biotechnol. Bioeng. 42:1993;430-439.
    • (1993) Biotechnol. Bioeng. , vol.42 , pp. 430-439
    • Shi, Y.1    Ryu, D.D.Y.2    Park, S.H.3
  • 50
    • 0029839957 scopus 로고    scopus 로고
    • Online measurement of oxygen-uptake in cell-culture using the dynamic method
    • Singh V. Online measurement of oxygen-uptake in cell-culture using the dynamic method. Biotechnol. Bioeng. 52:1996;443-448.
    • (1996) Biotechnol. Bioeng. , vol.52 , pp. 443-448
    • Singh, V.1
  • 53
    • 0020984996 scopus 로고
    • An examination of the data and concepts germane to the oxygenation of cultured animal cells
    • Copenhagen, Denmark. Karger, Basel
    • Spier, R.E., Griffiths, B., 1982. An examination of the data and concepts germane to the oxygenation of cultured animal cells. In: Proceedings of the 5th Meeting of ESACT, Copenhagen, Denmark. Karger, Basel, pp. 81-92.
    • (1982) In: Proceedings of the 5th Meeting of ESACT , pp. 81-92
    • Spier, R.E.1    Griffiths, B.2
  • 54
    • 0001251970 scopus 로고
    • Dynamic measurement of the volumetric oxygen transfer coefficient in fermentation systems
    • Taguchi H., Humphrey A.E. Dynamic measurement of the volumetric oxygen transfer coefficient in fermentation systems. J. Ferment. Technol. 44:1966;881-889.
    • (1966) J. Ferment. Technol. , vol.44 , pp. 881-889
    • Taguchi, H.1    Humphrey, A.E.2
  • 56
    • 0024017530 scopus 로고
    • The growth and productivity of recombinant animal cells in a bubble-free aeration system
    • Wagner R., Lehmann J. The growth and productivity of recombinant animal cells in a bubble-free aeration system. TIBTECH. 6:1988;101-104.
    • (1988) TIBTECH , vol.6 , pp. 101-104
    • Wagner, R.1    Lehmann, J.2
  • 57
    • 0025701107 scopus 로고
    • Relationship between oxygen consumption rate and cellular activity of mammalian cells cultured in serum-free media
    • Yamada K., Furushou S., Sugahara T., Shirahata S., Murakami H. Relationship between oxygen consumption rate and cellular activity of mammalian cells cultured in serum-free media. Biotechnol. Bioeng. 36:1990;759-762.
    • (1990) Biotechnol. Bioeng. , vol.36 , pp. 759-762
    • Yamada, K.1    Furushou, S.2    Sugahara, T.3    Shirahata, S.4    Murakami, H.5
  • 58
    • 0028483537 scopus 로고
    • Continuous, real-time monitoring of the oxygen-uptake rate (OUR) in animal-cell bioreactors
    • Yoon S., Konstantinov K.B. Continuous, real-time monitoring of the oxygen-uptake rate (OUR) in animal-cell bioreactors. Biotechnol. Bioeng. 44:1994;983-990.
    • (1994) Biotechnol. Bioeng. , vol.44 , pp. 983-990
    • Yoon, S.1    Konstantinov, K.B.2
  • 59
    • 0026662771 scopus 로고
    • Foaming and media surfactant effects on the cultivation of animal-cells in stirred and sparged bioreactors
    • Zhang S., Handacorrigan A., Spier R.E. Foaming and media surfactant effects on the cultivation of animal-cells in stirred and sparged bioreactors. J. Biotechnol. 25:1992;289-306.
    • (1992) J. Biotechnol. , vol.25 , pp. 289-306
    • Zhang, S.1    Handacorrigan, A.2    Spier, R.E.3
  • 60
    • 0028454939 scopus 로고
    • Online characterization of a hybridoma cell-culture process
    • Zhou W.C., Hu W.S. Online characterization of a hybridoma cell-culture process. Biotechnol. Bioeng. 44:1994;170-177.
    • (1994) Biotechnol. Bioeng. , vol.44 , pp. 170-177
    • Zhou, W.C.1    Hu, W.S.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.