Dissolved oxygen and pH profile evolution after cryovial thaw and repeated cell passaging in a T-75 flask

Biotechnology and Bioengineering

Volumn 105, Issue 6, 2010, Pages 1040-1047

  Vallejos, Jose R ^a   Brorson, Kurt A ^b   Moreira, Antonio R ^a   Rao, Govind ^a  

^a Baltimore County   (United States)

^b CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

Cell culture; Monitoring; Oxygen; Passaging; pH; T flask

Indexed keywords

BIOPROCESS DEVELOPMENT; BLACK BOXES; CELL CULTURE TECHNIQUES; METABOLIC STATE; ONLINE MONITORING; OXYGEN CONSUMPTION RATE; PASSAGING; PROCESS OPTIMIZATION; PROCESS VALIDATION; PRODUCTION RATES; PROFILE EVOLUTION; ROUTINE MONITORING; SCALE-UP; SEED CULTURES; SPECIFIC OXYGEN UPTAKE RATE;

BIOINFORMATICS; DISSOLUTION; DISSOLVED OXYGEN; DISSOLVED OXYGEN SENSORS; METABOLISM; OPTIMIZATION; SENSORS; THAWING; VOLTAGE MEASUREMENT;

CELL CULTURE;

DISSOLVED OXYGEN; GLUCOSE; LACTIC ACID;

ANIMAL CELL; ARTICLE; BIOPROCESS; CELL CULTURE; CELL PASSAGING; CRYOPRESERVATION; EVOLUTION; LABORATORY APPARATUS, EQUIPMENT AND SUPPLIES; MOUSE; NONHUMAN; OXYGEN CONSUMPTION; PH; T 75 FLASK; THAWING;

ANIMALS; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; FREEZING; GLUCOSE; HYDROGEN-ION CONCENTRATION; LACTIC ACID; MICE; MULTIPLE MYELOMA; OXYGEN;

EID: 77951549439     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22649     Document Type: Article

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