Online monitoring of oxygen in spinner flasks

Biotechnology Letters

Volumn 31, Issue 5, 2009, Pages 665-669

  Deshpande, Rahul Ravi ^a,b   Heinzle, Elmar ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b TU DORTMUND UNIVERSITY   (Germany)

Author keywords

Mammalian cell culture; Online O2 monitoring; Optical oxygen sensor; Oxygen transfer; Spinner flask

Indexed keywords

MAMMALIAN CELL CULTURE; ONLINE O2 MONITORING; OPTICAL OXYGEN SENSOR; OXYGEN TRANSFER; SPINNER FLASK;

ANIMAL CELL CULTURE; BOTTLES; CELL GROWTH; DISSOLUTION; GROWTH KINETICS; MAMMALS; OXYGEN; OXYGEN SENSORS; PARTIAL DISCHARGES; PROCESS MONITORING; SENSORS;

CELL CULTURE;

OXYGEN;

ARTICLE; CHEMISTRY; CULTURE MEDIUM; CULTURE TECHNIQUE; METHODOLOGY;

CELL CULTURE TECHNIQUES; CULTURE MEDIA; OXYGEN;

MAMMALIA;

EID: 65049083962     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-009-9919-2     Document Type: Article

References (11)

1. GV Annathur JL Pierce RG Combs AS Rathore A Banerjee DE Steinmeyer 2003 Improvements in spinner-flask designs for insect-cell suspension culture Biotechnol Appl Biochem 38 15 18
2. RR Deshpande E Heinzle 2004 On-line oxygen uptake rate and culture viability measurement of animal cell culture using microplates with integrated oxygen sensors Biotechnol Lett 26 763 767
3. Dunn IJ, Heinzle E, Ingham J, Prenosil JE (2003) Biological reaction engineering. Dynamic modelling fundamentals with simulation exercises, 2nd edn. Wiley-VCH, Weinheim
4. K Eyer E Heinzle 1996 On-line estimation of ATP and biomass production in hybridoma culture at various dissolved oxygen levels Biotechnol Bioeng 49 277 283
5. K Eyer A Oeggerli E Heinzle 1995 On-line gas analysis in animal cell cultivation: II. Oxygen uptake rate measurement and its application to controlled feeding of glutamate Biotechnol Bioeng 45 54 62
6. MA Hanson X Ge Y Kostov KA Brorson AR Moreira G Rao 2007 Comparisons of optical pH and dissolved oxygen sensors with traditional electrochemical probes during mammalian cell culture Biotechnol Bioeng 97 833 841
7. I Klimant OS Wolfbeis 1995 Oxygen-sensitive luminescent materials based on silicone-soluble ruthenium diimine complexes Anal Chem 67 3160 3166
8. AS Kocincová S Nagl S Arain C Krause SM Borisov M Arnold OS Wolfbeis 2008 Multiplex bacterial growth monitoring in 24-well microplates using a dual optical sensor for dissolved oxygen and pH Biotechnol Bioeng 100 430 438
9. A Oeggerli K Eyer E Heinzle 1995 On-line gas analysis in animal cell cultivation: I. Control of dissolved oxygen and pH Biotechnol Bioeng 45 42 53
10. 2 in shake-flasks Biotechnol Lett 25 377 380
11. C Zupke AJ Sinskey G Stephanopoulos 1995 Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas Appl Microbiol Biotechnol 44 27 36