Laboratory evolution of an epoxide hydrolase - Towards an enantioconvergent biocatalyst

Journal of Biotechnology

Volumn 156, Issue 1, 2011, Pages 1-10

  Kotik, Michael ^a   Archelas, Alain ^b   Faměrová, Veronika ^a   Oubrechtová, Pavla ^a   Křen, Vladimír ^a  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

^b AIX MARSEILLE UNIVERSITÉ   (France)

Author keywords

Deracemization; Directed evolution; Enantioconvergence; Enantioselectivity; Regioselectivity; Styrene oxide

Indexed keywords

ACTIVE SITE; ASPERGILLUS NIGER; CHIRAL BUILDING BLOCKS; CHIRAL GAS CHROMATOGRAPHY; DE-RACEMIZATION; DIRECTED EVOLUTION; ENANTIOCONVERGENCE; ENANTIOMERIC EXCESS; EPOXIDE HYDROLASES; MUTANT ENZYMES; RACEMIC SUBSTRATES; SATURATION MUTAGENESIS; STYRENE OXIDE; VICINAL DIOLS; WILD TYPES;

AMINO ACIDS; ASPERGILLUS; ENANTIOSELECTIVITY; ETHYLENE GLYCOL; GAS CHROMATOGRAPHY; HYDROLASES; MUTAGENESIS; REGIOSELECTIVITY; STYRENE; SUBSTRATES;

ENANTIOMERS;

ENZYME VARIANT; EPOXIDE HYDROLASE; FUNGAL ENZYME; STYRENE OXIDE;

AMINO ACID TRANSPORT; ARTICLE; ASPERGILLUS NIGER; BINDING SITE; BIOCATALYST; CHIRAL CHROMATOGRAPHY; CONVERGENT EVOLUTION; ENANTIOMER; ENANTIOSELECTIVITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME MECHANISM; ENZYME SUBSTRATE; ENZYME SYNTHESIS; GAS CHROMATOGRAPHY; IN VITRO STUDY; LABORATORY; MUTAGEN TESTING; MUTAGENESIS; PRIORITY JOURNAL; RACEMIC MIXTURE;

ASPERGILLUS NIGER; BACTERIAL PROTEINS; CATALYSIS; CATALYTIC DOMAIN; EPOXIDE HYDROLASES; EPOXY COMPOUNDS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; STEREOISOMERISM; STRUCTURE-ACTIVITY RELATIONSHIP;

ASPERGILLUS NIGER;

EID: 80052884406     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2011.08.003     Document Type: Article

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