Modification of Activity and Specificity of Haloalkane Dehalogenase from Sphingomonas paucimobilis UT26 by Engineering of Its Entrance Tunnel

Journal of Biological Chemistry

Volumn 278, Issue 52, 2003, Pages 52622-52628

  Chaloupková, Radka ^a   Sýkorová, Jana ^a   Prokop, Zbyňek ^a   Jesenská, Andrea ^a   Monincová, Marta ^a   Pavlová, Martina ^a   Tsuda, Masataka ^b   Nagata, Yuji ^b   Damborský, Jiří ^b  

^a MASARYK UNIVERSITY   (Czech Republic)

^b TOHOKU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BACTERIA; CATALYSIS; ENZYMES; GENES; MUTAGENESIS; SUBSTRATES;

MULTIVARIATE ANALYSIS; MUTATIONS;

BIOCHEMISTRY;

ALANINE; BACTERIAL ENZYME; GLYCINE; HALOALKANE DEHALOGENASE; LEUCINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; COMPARATIVE STUDY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME MODIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; MULTIVARIATE ANALYSIS; NONHUMAN; PHYLOGENY; PRIORITY JOURNAL; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS; SPHINGOMONAS PAUCIMOBILIS; STRUCTURE ANALYSIS;

ALANINE; AMINO ACIDS; BINDING SITES; CATALYSIS; CATALYTIC DOMAIN; CIRCULAR DICHROISM; ESCHERICHIA COLI; GLYCINE; HYDROLASES; KINETICS; LEUCINE; MODELS, MOLECULAR; MULTIVARIATE ANALYSIS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHYLOGENY; PROTEIN FOLDING; SPHINGOMONAS; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; SPHINGOMONAS; SPHINGOMONAS PAUCIMOBILIS;

EID: 0346101759     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M306762200     Document Type: Article

References (43)

1. Janssen, D. B., Pries, F., and Van der Ploeg, J. R. (1994) Annu. Rev. Microbiol. 48, 163-191
2. Swanson, P. E. (1999) Curr. Opin. Biotechnol. 10, 365-369
3. Schanstra, J. P., Ridder, A., Kingma, J., and Janssen, D. B. (1997) Protein Eng. 10, 53-61
4. Schanstra, J. P., Ridder, I. S., Heimeriks, G. J., Rink, R., Poelarends, G. J., Kalk, K. H., Dijkstra, B. W., and Janssen, D. B. (1996) Biochemistry 35, 13186-13195
5. Holloway, P., Knoke, K. L., Trevors, J. T., and Lee, H. (1998) Biotechnol. Bioeng. 59, 520-523
6. Hynkova, K., Nagata, Y., Takagi, M., and Damborsky, J. (1999) FEBS Lett. 446, 177-181
7. Schindler, J. F., Naranjo, P. A., Honaberger, D. A., Chang, C.-H., Brainard, J. R., Vanderberg, L. A., and Unkefer, C. J. (1999) Biochemistry 38, 5772-5778
8. Marvanova, S., Nagata, Y., Wimmerova, M., Sykorova, J., Hynkova, K., and Damborsky, J. (2001) J. Microbiol. Methods 44, 149-157
9. Gray, K. A., Richardson, T. H., Kretz, K., Short, J. M., Bartnek, F., Knowles, R., Kan, L., Swanson, P. E., and Robertson, D. E. (2001) Adv. Synthesis Catalysis 343, 607-616
10. Pikkemaat, M. G., and Janssen, D. B. (2002) Nucleic Acids Res. 30, 35-39
11. Bosma, T., Damborsky, J., Stucki, G., and Janssen, D. B. (2002) Appl. Environ. Microbiol. 68, 3582-3587
12. Bohac, M., Nagata, Y., Prokop, Z., Prokop, M., Monincova, M., Koca, J., Tsuda, M., and Damborsky, J. (2002) Biochemistry 41, 14272-14280
13. Nagata, Y., Prokop, Z., Marvanova, S., Sykorova, J., Monincova, M., Tsuda, M., and Damborsky, J. (2003) Appl. Environ. Microbiol. 69, 2349-2355
14. Stucki, G., and Thuer, M. (1995) Environ. Sci. Technol. 29, 2339-2345
15. Dravis, B. C., LeJeune, K. E., Hetro, A. D., and Russell, A. J. (2000) Biotechnol. Bioeng. 69, 235-241
16. Dravis, B. C., Swanson, P. E., and Russell, A. J. (2001) Biotechnol. Bioeng. 75, 416-423
17. Damborsky, J., Kuty, M., Nemec, M., and Koca, J. (1997) J. Chem. Information Comput. Sci. 37, 562-568
18. Lightstone, F. C., Zheng, Y. J., and Bruice, T. C. (1998) J. Am. Chem. Soc. 120, 5611-5621
19. Damborsky, J., and Koca, J. (1999) Protein Eng. 12, 989-998
20. Lau, E. Y., Kahn, K., Bash, P., and Bruice, T. C. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 9937-9942
21. Lewandowicz, A., Rudzinski, J., Tronstad, L., Widersten, M., Ryberg, P., Matsson, O., and Paneth, P. (2001) J. Am. Chem. Soc. 123, 4550-4555
22. Otyepka, M., and Damborsky, J. (2002) Protein Sci. 11, 1206-1217
23. Shurki, A., Strajbl, M., Villa, J., and Warshel, A. (2002) J. Am. Chem. Soc. 124, 4097-4107
24. Devi-Kesavan, L. S., and Gao, J. (2003) J. Am. Chem. Soc. 125, 1532-1540
25. Nagata, Y., Miyauchi, K., Damborsky, J., Manova, K., Ansorgova, A., and Takagi, M. (1997) Appl. Environ. Microbiol. 63, 3707-3710
26. Nagata, Y., Miyauchi, K., and Takagi, M. (1999) J. Industrial Microbiol. Biotechnol. 23, 380-390
27. Damborsky, J., Rorije, E., Jesenska, A., Nagata, Y., Klopman, G., and Peijnenburg, W. J. G. M. (2001) Environ. Toxicol. Chem. 20, 2681-2689
28. Verschueren, K. H. G., Seljee, F., Rozeboom, H. J., Kalk, K. H., and Dijkstra, B. W. (1993) Nature 363, 693-698
29. Newman, J., Peat, T. S., Richard, R., Kan, L., Swanson, P. E., Affholter, J. A., Holmes, I. H., Schindler, J. F., Unkefer, C. J., and Terwilliger, T. C. (1999) Biochemistry 38, 16105-16114
30. Marek, J., Vevodova, J., Kuta-Smatanova, I., Nagata, Y., Svensson, L. A., Newman, J., Takagi, M., and Damborsky, J. (2000) Biochemistry 39, 14082-14086
31. Jesenska, A., Bartos, M., Czernekova, V., Rychlik, I., Pavlik, I., and Damborsky, J. (2002) Appl. Environ. Microbiol. 68, 3724-3730
32. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D. J. (1997) Nucleic Acids Res. 25, 3389-3402
33. Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) Nucleic Acids Res. 22, 4673-4680
34. Nagata, Y., Hynkova, K., Damborsky, J., and Takagi, M. (1999) Protein Expression Purif. 17, 299-304
35. Wold, S., Esbensen, K., and Geladi, P. (1987) Chemometrics Intelligent Lab. Syst. 2, 37-52
36. Damborsky, J., Nyandoroh, M. G., Nemec, M., Holoubek, I., Bull, A. T., and Hardman, D. J. (1997) Biotechnol. Appl. Biochem. 26, 19-25
37. Oakley, A. J., Prokop, Z., Bohac, M., Kmunicek, J., Jedlicka, T., Monincova, M., Kuta-Smatanova, I., Nagata, Y., Damborsky, J., and Wilce, M. C. J. (2002) Biochemistry 41, 4847-4855
38. Arnold, F. H. (1998) Acc. Chem. Res. 31, 125-131
39. Arnold, F. H. (1998) Nat. Biotechnol. 16, 617-618
40. Huang, X., and Raushel, F. M. (2000) Biochemistry 39, 3240-3247
41. Capila, I., Wu, Y., Rethwisch, D. W., Matte, A., Cygler, M., and Linhardt, R. J. (2002) Biochim. Biophys. Acta 1597, 260-270
42. Schmitt, J., Brocca, S., Schmid, R. D., and Pleiss, J. (2002) Protein Eng. 15, 595-601
43. Damborsky, J. (1998) Protein Eng. 11, 21-30