The use of site-directed mutagenesis to study GPCRs

Methods in Molecular Biology

Volumn 746, Issue , 2011, Pages 85-98

  Conner, Alex C ^a   Barwell, James ^b   Poyner, David R ^c   Wheatley, Mark ^d  

^a UNIVERSITY OF WARWICK   (United Kingdom)

^b UNIVERSITY OF WARWICK   (United Kingdom)

^c ASTON UNIVERSITY   (United Kingdom)

^d UNIVERSITY OF BIRMINGHAM   (United Kingdom)

Author keywords

Alanine scan; Chimeric mutagenesis; Plasmid amplification; Plasmid transfection; Primer design; Random mutagenesis; Substituted cysteine accessibility method

Indexed keywords

ALANINE; G PROTEIN COUPLED RECEPTOR; OLIGONUCLEOTIDE; CHIMERIC PROTEIN;

ARTICLE; BACTERIAL CELL; CELL CULTURE; CHIMERA; CONTROLLED STUDY; GENE AMPLIFICATION; GENETIC TRANSFECTION; MAMMAL CELL; MELTING POINT; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN FUNCTION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; GENE VECTOR; GENETICS; METABOLISM;

CELLS, CULTURED; GENETIC VECTORS; MUTAGENESIS, SITE-DIRECTED; MUTANT CHIMERIC PROTEINS; RECEPTORS, G-PROTEIN-COUPLED; TRANSFECTION;

EID: 80052819103     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-126-0_5     Document Type: Article

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