Techniques: How to boost GPCR mutagenesis studies using yeast

Trends in Pharmacological Sciences

Volumn 26, Issue 10, 2005, Pages 533-539

  Beukers, Margot W ^a   Ijzerman, Adriaan P ^a  

^a LEIDEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; BETA GALACTOSIDASE; CYSTEINE; G PROTEIN COUPLED RECEPTOR; LIGAND;

ALANINE CYSTEINE SCANNING MUTAGENESIS; AMINO ACID SUBSTITUTION; BIOENGINEERING; HIGH THROUGHPUT SCREENING; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN STRUCTURE; RECEPTOR SELECTION AND AMPLIFICATION TECHNOLOGY; REVIEW; SATURATION MUTAGENESIS; SCREENING TEST; SITE DIRECTED MUTAGENESIS; TECHNIQUE; YEAST;

ANIMALS; HUMANS; MOLECULAR BIOLOGY; MUTAGENESIS; RECEPTORS, G-PROTEIN-COUPLED; SIGNAL TRANSDUCTION; YEASTS;

EID: 25144437063     PISSN: 01656147     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tips.2005.08.005     Document Type: Review

References (59)

1. E.S. Lander Initial sequencing and analysis of the human genome Nature 409 2001 860 921
2. J.C. Venter The sequence of the human genome Science 291 2001 1304 1351
3. Y. Inoue Proteome-wide classification and identification of mammalian-type GPCRs by binary topology pattern Comput. Biol. Chem. 28 2004 39 49
4. J. Drews Drug discovery: a historical perspective Science 287 2000 1960 1964
5. Ø. Edvardsen tGRAP, the G-protein coupled receptors mutant database Nucleic Acids Res. 30 2002 361 363
6. F. Horn GPCRDB: an information system for G protein-coupled receptors Nucleic Acids Res. 26 1998 275 279
7. F. Horn Automated extraction of mutation data from the literature: application of MuteXt to G protein-coupled receptors and nuclear hormone receptors Bioinformatics 20 2004 557 568
8. K. Palczewski Crystal structure of rhodopsin: a G protein-coupled receptor Science 289 2000 739 745
9. Z.L. Lu Seven-transmembrane receptors: crystals clarify Trends Pharmacol. Sci. 23 2002 140 146
10. K. Kristiansen Molecular mechanisms of ligand binding, signaling, and regulation within the superfamily of G-protein-coupled receptors: molecular modeling and mutagenesis approaches to receptor structure and function Pharmacol. Ther. 103 2004 21 80
11. 2+ J. Biol. Chem. 280 2005 5113 5120
12. G.K. Dhami Phosphorylation-independent regulation of metabotropic glutamate receptor 1 signaling requires G protein-coupled receptor kinase 2 binding to the second intracellular loop J. Biol. Chem. 280 2005 24420 24427
13. W.L. Hubbell Rhodopsin structure, dynamics, and activation: a perspective from crystallography, site-directed spin labeling, sulfhydryl reactivity, and disulfide cross-linking Adv. Protein Chem. 63 2003 243 290
14. G.A. Weiss Rapid mapping of protein functional epitopes by combinatorial alanine scanning Proc. Natl. Acad. Sci. U. S. A. 97 2000 8950 8954
15. T.A. Kunkel Rapid and efficient site-specific mutagenesis without phenotypic selection Methods Enzymol. 154 1987 367 382
16. J.F. Smothers Phage display: affinity selection from biological libraries Science 298 2002 621 622
17. M. Szardenings Phage display of random peptide libraries: applications, limits, and potential J. Recept. Signal Transduct. Res. 23 2003 307 349
18. C.M. Sommers, and M.E. Dumont Genetic interactions among the transmembrane segments of G Protein Coupled Receptor encoded by the yeast STE2 gene J. Mol. Biol. 266 1997 559 575
19. N.P. Martin Mutagenic mapping of helical structures in the transmembrane segments of the yeast α factor receptor J. Mol. Biol. 317 2002 765 788
20. A. Celic Sequences in the intracellular loops of the yeast pheromone receptor Ste2p required for G protein activation Biochemistry 42 2003 3004 3017
21. J.L.S. Milne Random mutagenesis of the cAMP chemoattractant receptor, cAR1, of Dictyostelium J. Biol. Chem. 272 1997 2069 2076
22. T.J. Baranski C5a receptor activation. Genetic identification of critical residues in four transmembrane helices J. Biol. Chem. 274 1999 15757 15765
23. A. Geva Genetic mapping of the human C5a receptor. Identification of transmembrane amino acids critical for receptor function J. Biol. Chem. 275 2000 35393 35401
24. J.M. Klco Essential role for the second extracellular loop in C5a receptor activation Nat. Struct. Mol. Biol. 12 2005 320 326
25. I. Erlenbach Single amino acid substitutions and deletions that alter the G protein coupling properties of the V2 vasopressin receptor identified in yeast by receptor random mutagenesis J. Biol. Chem. 276 2001 29382 29392
26. A. Celic Intensive mutational analysis of G protein-coupled receptors in yeast Methods Mol. Biol. 237 2004 105 120
27. M. Fromant Direct random mutagenesis of gene-sized DNA fragments using polymerase chain reaction Anal. Biochem. 224 1995 347 353
28. 2B receptor followed by growth selection in yeast. Identification of constitutively active and gain of function mutations Mol. Pharmacol. 65 2004 702 710
29. G. Chao Fine epitope mapping of anti-epidermal growth factor receptor antibodies through random mutagenesis and yeast surface display J. Mol. Biol. 342 2004 539 550
30. J.S. Gutkind Muscarinic acetylcholine receptor subtypes as agonist-dependent oncogenes Proc. Natl. Acad. Sci. U. S. A. 88 1991 4703 4707
31. D.J. Ford Homologous mutations near the junction of the sixth transmembrane domain and the third extracellular loop lead to constitutive activity and enhanced agonist affinity at all muscarinic receptor subtypes J. Pharmacol. Exp. Ther. 300 2002 810 817
32. M.J. Smit G protein-coupled receptors and proliferative signaling Methods Enzymol. 343 2002 430 447
33. J.T. Weissman G-protein-coupled receptor-mediated activation of rap GTPases: characterization of a novel Galphai regulated pathway Oncogene 23 2004 241 249
34. E.S. Burstein The second intracellular loop of the m5 muscarinic receptor is the switch which enables G-protein coupling J. Biol. Chem. 273 1998 24322 24327
35. A.A. Jensen Functional importance of the Ala116-Pro136 region in the calcium-sensing receptor J. Biol. Chem. 275 2000 29547 29555
36. G. Ladds Modified yeast cells to investigate the coupling of G protein-coupled receptors to specific proteins Mol. Microbiol. 47 2003 781 792
37. G. Ladds, and J. Davey Analysis of human GPCRs in fission yeast Curr. Opin. Drug Discov. Devel. 7 2004 683 691
38. S. Macauley-Patrick Heterologous protein production using the Pichia pastoris expression system Yeast 22 2005 249 270
39. M.H. Pausch G-protein-coupled receptors in Saccharomyces cerevisiae: high-throughput screening assays for drug discovery Trends Biotechnol. 15 1997 487 494
40. C. Klein Identification of surrogate agonists for the human FPRL-1 receptor by autocrine selection in yeast Nat. Biotechnol. 16 1998 1334 1337
41. N.S. Olesnicky A constitutively active G-protein-coupled receptor causes mating self-compatibility in the mushroom Coprinus EMBO J. 18 1999 2756 2763
42. A.J. Brown Functional coupling of mammalian receptors to the yeast mating pathway using novel yeast/mammalian G protein alpha-subunit chimeras Yeast 16 2000 11 22
43. C.M. Sommers A limited spectrum of mutations causes constitutive activation of the yeast alpha-factor receptor Biochemistry 39 2000 6898 6909
44. W.B. Zhang A point mutation that confers constitutive activity to CXCR4 reveals that T140 is an inverse agonist and that AMD3100 and ALX40-4C are weak partial agonists J. Biol. Chem. 277 2002 24515 24521
45. D.A. Arias Constitutive activation of CCR5 and CCR2 induced by conformational changes in the conserved TXP motif in transmembrane helix 2 J. Biol. Chem. 278 2003 36513 36521
46. 5 muscarinic acetylcholine receptors in yeast J. Neurochem. 77 2001 1327 1337
47. 3 receptor and greatly impair receptor/G protein coupling J. Biol. Chem. 278 2003 30248 30260
48. S.J. Dowell, and A.J. Brown Yeast assays for G-protein-coupled receptors Receptors Channels 8 2002 343 352
49. J.P. Manfredi Yeast alpha mating factor structure-activity relationship derived from genetically selected peptide agonists and antagonists of Ste2p Mol. Cell. Biol. 16 1996 4700 4709
50. R.T. Bass Identification and characterization of novel somatostatin antagonists Mol. Pharmacol. 50 1996 709 715
51. E. Pajot-Augy Engineered Yeasts as reporter systems for odorant detection J. Recept. Signal Transduct. Res. 23 2003 155 171
52. A.J. Brown The Orphan G protein-coupled receptors GPR41 and GPR43 are activated by propionate and other short chain carboxylic acids J. Biol. Chem. 278 2003 11312 11319
53. A. Wise Target validation of G-protein coupled receptors Drug Discov. Today 7 2002 235 246
54. 3 receptor J. Biol. Chem. 280 2005 5664 5675
55. J.C. Lin Identification of residues that contribute to receptor activation through the analysis of compensatory mutations in the G protein-coupled α-factor receptor Biochemistry 44 2005 1278 1287
56. M. Versele Sex and sugar in yeast: two distinct GPCR systems EMBO Rep. 2 2001 574 579
57. K. Lemaire Glucose and sucrose act as agonist and mannose as antagonist ligands of the G Protein-Coupled Receptor Gpr1 in the yeast Saccharomyces cerevisiae Mol. Cell 16 2004 293 299
58. H. Tamaki Glucose-dependent cell size is regulated by a G protein-coupled receptor system in yeast Saccharomyces cerevisiae Genes Cells 10 2005 193 206
59. M. Eilers Comparison of class A and D G protein-coupled receptors: common features in structure and activation Biochemistry 44 2005 8959 8975