메뉴 건너뛰기




Volumn 278, Issue 18, 2011, Pages 3431-3446

Intein lacking conserved C-terminal motif G retains controllable N-cleavage activity

Author keywords

N cleavage; peptide complementation; protein engineering; split intein; thiolysis

Indexed keywords

C INTEIN; HYBRID PROTEIN; INTEIN; N INTEIN; PEPTIDE DERIVATIVE; SERINE; UNCLASSIFIED DRUG;

EID: 80052469458     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2011.08266.x     Document Type: Article
Times cited : (9)

References (50)
  • 2
    • 0034602984 scopus 로고    scopus 로고
    • Dissecting the chemistry of protein splicing and its applications
    • Noren CJ, Wang J, &, Perler FB, (2000) Dissecting the chemistry of protein splicing and its applications. Angew Chem Int Ed Engl 39, 450-466.
    • (2000) Angew Chem Int Ed Engl , vol.39 , pp. 450-466
    • Noren, C.J.1    Wang, J.2    Perler, F.B.3
  • 3
    • 0141755113 scopus 로고    scopus 로고
    • Crystal structure of a mini-intein reveals a conserved catalytic module involved in side chain cyclization of asparagine during protein splicing
    • Ding Y, Xu MQ, Ghosh I, Chen X, Ferrandon S, Lesage G, &, Rao Z, (2003) Crystal structure of a mini-intein reveals a conserved catalytic module involved in side chain cyclization of asparagine during protein splicing. J Biol Chem 278, 39133-39142.
    • (2003) J Biol Chem , vol.278 , pp. 39133-39142
    • Ding, Y.1    Xu, M.Q.2    Ghosh, I.3    Chen, X.4    Ferrandon, S.5    Lesage, G.6    Rao, Z.7
  • 4
    • 27144547019 scopus 로고    scopus 로고
    • Crystal structures of an intein from the split dnaE gene of Synechocystis sp. PCC6803 reveal the catalytic model without the penultimate histidine and the mechanism of zinc ion inhibition of protein splicing
    • Sun P, Ye S, Ferrandon S, Evans TC, Xu MQ, &, Rao Z, (2005) Crystal structures of an intein from the split dnaE gene of Synechocystis sp. PCC6803 reveal the catalytic model without the penultimate histidine and the mechanism of zinc ion inhibition of protein splicing. J Mol Biol 353, 1093-1105.
    • (2005) J Mol Biol , vol.353 , pp. 1093-1105
    • Sun, P.1    Ye, S.2    Ferrandon, S.3    Evans, T.C.4    Xu, M.Q.5    Rao, Z.6
  • 5
    • 0031975772 scopus 로고    scopus 로고
    • Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing
    • Klabunde T, Sharma S, Telenti A, Jacobs WR Jr, &, Sacchettini JC, (1998) Crystal structure of GyrA intein from Mycobacterium xenopi reveals structural basis of protein splicing. Nat Struct Biol 5, 31-36.
    • (1998) Nat Struct Biol , vol.5 , pp. 31-36
    • Klabunde, T.1    Sharma, S.2    Telenti, A.3    Jacobs, Jr.W.R.4    Sacchettini, J.C.5
  • 6
    • 0034595699 scopus 로고    scopus 로고
    • Structural insights into the protein splicing mechanism of PI-SceI
    • Poland BW, Xu MQ, &, Quiocho FA, (2000) Structural insights into the protein splicing mechanism of PI-SceI. J Biol Chem 275, 16408-16413.
    • (2000) J Biol Chem , vol.275 , pp. 16408-16413
    • Poland, B.W.1    Xu, M.Q.2    Quiocho, F.A.3
  • 7
    • 0030763705 scopus 로고    scopus 로고
    • Compilation and analysis of intein sequences
    • Perler FB, Olsen GJ, &, Adam E, (1997) Compilation and analysis of intein sequences. Nucleic Acids Res 25, 1087-1093.
    • (1997) Nucleic Acids Res , vol.25 , pp. 1087-1093
    • Perler, F.B.1    Olsen, G.J.2    Adam, E.3
  • 8
    • 0033814098 scopus 로고    scopus 로고
    • Fusions to self-splicing inteins for protein purification
    • Xu MQ, Paulus H, &, Chong S, (2000) Fusions to self-splicing inteins for protein purification. Methods Enzymol 326, 376-418.
    • (2000) Methods Enzymol , vol.326 , pp. 376-418
    • Xu, M.Q.1    Paulus, H.2    Chong, S.3
  • 9
    • 0037208835 scopus 로고    scopus 로고
    • Purification of recombinant proteins from E. coli by engineered inteins
    • Xu MQ, &, Evans TC Jr, (2003) Purification of recombinant proteins from E. coli by engineered inteins. Methods Mol Biol 205, 43-68.
    • (2003) Methods Mol Biol , vol.205 , pp. 43-68
    • Xu, M.Q.1    Evans, Jr.T.C.2
  • 10
    • 0037548053 scopus 로고    scopus 로고
    • Semisynthesis of proteins by expressed protein ligation
    • Muir TW, (2003) Semisynthesis of proteins by expressed protein ligation. Annu Rev Biochem 72, 249-289.
    • (2003) Annu Rev Biochem , vol.72 , pp. 249-289
    • Muir, T.W.1
  • 11
    • 70849134554 scopus 로고    scopus 로고
    • Expressed protein ligation: A resourceful tool to study protein structure and function
    • Berrade L, &, Camarero JA, (2009) Expressed protein ligation: a resourceful tool to study protein structure and function. Cell Mol Life Sci 66, 3909-3922.
    • (2009) Cell Mol Life Sci , vol.66 , pp. 3909-3922
    • Berrade, L.1    Camarero, J.A.2
  • 12
    • 32044468517 scopus 로고    scopus 로고
    • Protein trans-splicing and characterization of a split family B-type DNA polymerase from the hyperthermophilic archaeal parasite Nanoarchaeum equitans
    • Choi JJ, Nam KH, Min B, Kim SJ, Soll D, &, Kwon ST, (2006) Protein trans-splicing and characterization of a split family B-type DNA polymerase from the hyperthermophilic archaeal parasite Nanoarchaeum equitans. J Mol Biol 356, 1093-1106.
    • (2006) J Mol Biol , vol.356 , pp. 1093-1106
    • Choi, J.J.1    Nam, K.H.2    Min, B.3    Kim, S.J.4    Soll, D.5    Kwon, S.T.6
  • 13
    • 0032483013 scopus 로고    scopus 로고
    • Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803
    • Wu H, Hu Z, &, Liu XQ, (1998) Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803. Proc Natl Acad Sci USA 95, 9226-9231.
    • (1998) Proc Natl Acad Sci USA , vol.95 , pp. 9226-9231
    • Wu, H.1    Hu, Z.2    Liu, X.Q.3
  • 15
    • 18144439565 scopus 로고    scopus 로고
    • Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein
    • Wu H, Xu MQ, &, Liu XQ, (1998) Protein trans-splicing and functional mini-inteins of a cyanobacterial dnaB intein. Biochim Biophys Acta 1387, 422-432.
    • (1998) Biochim Biophys Acta , vol.1387 , pp. 422-432
    • Wu, H.1    Xu, M.Q.2    Liu, X.Q.3
  • 16
    • 33847249392 scopus 로고    scopus 로고
    • Trans-splicing of an artificially split fungal mini-intein
    • Elleuche S, &, Poggeler S, (2007) Trans-splicing of an artificially split fungal mini-intein. Biochem Biophys Res Commun 355, 830-834.
    • (2007) Biochem Biophys Res Commun , vol.355 , pp. 830-834
    • Elleuche, S.1    Poggeler, S.2
  • 17
    • 65249091343 scopus 로고    scopus 로고
    • In vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteins
    • Aranko AS, Züger S, Buchinger E, &, Iwai H, (2009) In vivo and in vitro protein ligation by naturally occurring and engineered split DnaE inteins. PLoS ONE 4, e5185.
    • (2009) PLoS ONE , vol.4
    • Aranko, A.S.1    Züger, S.2    Buchinger, E.3    Iwai, H.4
  • 19
    • 4143058067 scopus 로고    scopus 로고
    • Synthetic two-piece and three-piece split inteins for protein trans-splicing
    • Sun W, Yang J, &, Liu XQ, (2004) Synthetic two-piece and three-piece split inteins for protein trans-splicing. J Biol Chem 279, 35281-35286.
    • (2004) J Biol Chem , vol.279 , pp. 35281-35286
    • Sun, W.1    Yang, J.2    Liu, X.Q.3
  • 20
    • 70350533119 scopus 로고    scopus 로고
    • Split inteins as versatile tools for protein semisynthesis
    • Mootz HD, (2009) Split inteins as versatile tools for protein semisynthesis. Chembiochem 10, 2579-2589.
    • (2009) Chembiochem , vol.10 , pp. 2579-2589
    • Mootz, H.D.1
  • 21
    • 0035814803 scopus 로고    scopus 로고
    • Characterization of a naturally occurring trans-splicing intein from Synechocystis sp PCC6803
    • Martin DD, Xu MQ, &, Evans TC Jr, (2001) Characterization of a naturally occurring trans-splicing intein from Synechocystis sp PCC6803. Biochemistry 40, 1393-1402.
    • (2001) Biochemistry , vol.40 , pp. 1393-1402
    • Martin, D.D.1    Xu, M.Q.2    Evans, Jr.T.C.3
  • 22
    • 0037881919 scopus 로고    scopus 로고
    • Zinc ion effects on individual Ssp DnaE intein splicing steps: Regulating pathway progression
    • Nichols NM, Benner JS, Martin DD, &, Evans TC Jr, (2003) Zinc ion effects on individual Ssp DnaE intein splicing steps: regulating pathway progression. Biochemistry 42, 5301-5311.
    • (2003) Biochemistry , vol.42 , pp. 5301-5311
    • Nichols, N.M.1    Benner, J.S.2    Martin, D.D.3    Evans, Jr.T.C.4
  • 23
    • 65249150809 scopus 로고    scopus 로고
    • Novel split intein for trans-splicing synthetic peptide onto C terminus of protein
    • Appleby JH, Zhou K, Volkmann G, &, Liu XQ, (2009) Novel split intein for trans-splicing synthetic peptide onto C terminus of protein. J Biol Chem 284, 6194-6199.
    • (2009) J Biol Chem , vol.284 , pp. 6194-6199
    • Appleby, J.H.1    Zhou, K.2    Volkmann, G.3    Liu, X.Q.4
  • 24
    • 77954058847 scopus 로고    scopus 로고
    • Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein
    • Volkmann G, &, Liu XQ, (2009) Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein. PLoS ONE 4, e8381.
    • (2009) PLoS ONE , vol.4
    • Volkmann, G.1    Liu, X.Q.2
  • 26
    • 0035815658 scopus 로고    scopus 로고
    • Reversible inhibition of protein splicing by zinc ion
    • Mills KV, &, Paulus H, (2001) Reversible inhibition of protein splicing by zinc ion. J Biol Chem 276, 10832-10838.
    • (2001) J Biol Chem , vol.276 , pp. 10832-10838
    • Mills, K.V.1    Paulus, H.2
  • 27
    • 0041854719 scopus 로고    scopus 로고
    • Conditional protein splicing: A new tool to control protein structure and function in vitro and in vivo
    • Mootz HD, Blum ES, Tyszkiewicz AB, &, Muir TW, (2003) Conditional protein splicing: a new tool to control protein structure and function in vitro and in vivo. J Am Chem Soc 125, 10561-10569.
    • (2003) J Am Chem Soc , vol.125 , pp. 10561-10569
    • Mootz, H.D.1    Blum, E.S.2    Tyszkiewicz, A.B.3    Muir, T.W.4
  • 28
    • 0032562685 scopus 로고    scopus 로고
    • Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein
    • Chong S, Williams KS, Wotkowicz C, &, Xu MQ, (1998) Modulation of protein splicing of the Saccharomyces cerevisiae vacuolar membrane ATPase intein. J Biol Chem 273, 10567-10577.
    • (1998) J Biol Chem , vol.273 , pp. 10567-10577
    • Chong, S.1    Williams, K.S.2    Wotkowicz, C.3    Xu, M.Q.4
  • 29
    • 0032988320 scopus 로고    scopus 로고
    • Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein
    • 116, 118-120.
    • Southworth MW, Amaya K, Evans TC, Xu MQ, &, Perler FB, (1999) Purification of proteins fused to either the amino or carboxy terminus of the Mycobacterium xenopi gyrase A intein. BioTechniques 27, 110-114, 116, 118-120.
    • (1999) BioTechniques , vol.27 , pp. 110-114
    • Southworth, M.W.1    Amaya, K.2    Evans, T.C.3    Xu, M.Q.4    Perler, F.B.5
  • 30
    • 0030012109 scopus 로고    scopus 로고
    • Protein splicing: Evidence for an N-O acyl rearrangement as the initial step in the splicing process
    • Shao Y, Xu MQ, &, Paulus H, (1996) Protein splicing: evidence for an N-O acyl rearrangement as the initial step in the splicing process. Biochemistry 35, 3810-3815.
    • (1996) Biochemistry , vol.35 , pp. 3810-3815
    • Shao, Y.1    Xu, M.Q.2    Paulus, H.3
  • 31
    • 0029838842 scopus 로고    scopus 로고
    • The mechanism of protein splicing and its modulation by mutation
    • Xu MQ, &, Perler FB, (1996) The mechanism of protein splicing and its modulation by mutation. EMBO J 15, 5146-5153.
    • (1996) EMBO J , vol.15 , pp. 5146-5153
    • Xu, M.Q.1    Perler, F.B.2
  • 32
    • 0035968177 scopus 로고    scopus 로고
    • Zinc inhibition of protein trans-splicing and identification of regions essential for splicing and association of a split intein
    • Ghosh I, Sun L, &, Xu MQ, (2001) Zinc inhibition of protein trans-splicing and identification of regions essential for splicing and association of a split intein. J Biol Chem 276, 24051-24058.
    • (2001) J Biol Chem , vol.276 , pp. 24051-24058
    • Ghosh, I.1    Sun, L.2    Xu, M.Q.3
  • 33
    • 0033614484 scopus 로고    scopus 로고
    • Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: Facile production of protein building blocks for protein ligation
    • Mathys S, Evans TC, Chute IC, Wu H, Chong S, Benner J, Liu XQ, &, Xu MQ, (1999) Characterization of a self-splicing mini-intein and its conversion into autocatalytic N- and C-terminal cleavage elements: facile production of protein building blocks for protein ligation. Gene 231, 1-13.
    • (1999) Gene , vol.231 , pp. 1-13
    • Mathys, S.1    Evans, T.C.2    Chute, I.C.3    Wu, H.4    Chong, S.5    Benner, J.6    Liu, X.Q.7    Xu, M.Q.8
  • 34
    • 0344351815 scopus 로고    scopus 로고
    • Semisynthesis of cytotoxic proteins using a modified protein splicing element
    • Evans TC Jr, Benner J, &, Xu MQ, (1998) Semisynthesis of cytotoxic proteins using a modified protein splicing element. Protein Sci 7, 2256-2264.
    • (1998) Protein Sci , vol.7 , pp. 2256-2264
    • Evans, Jr.T.C.1    Benner, J.2    Xu, M.Q.3
  • 35
    • 17744415288 scopus 로고    scopus 로고
    • Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
    • et al.
    • Chong S, Mersha FB, Comb DG, Scott ME, Landry D, Vence LM, Perler FB, Benner J, Kucera RB, Hirvonen CA, et al. (1997) Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. Gene 192, 271-281.
    • (1997) Gene , vol.192 , pp. 271-281
    • Chong, S.1    Mersha, F.B.2    Comb, D.G.3    Scott, M.E.4    Landry, D.5    Vence, L.M.6    Perler, F.B.7    Benner, J.8    Kucera, R.B.9    Hirvonen, C.A.10
  • 36
    • 0035913755 scopus 로고    scopus 로고
    • Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins
    • Zhang A, Gonzalez SM, Cantor EJ, &, Chong S, (2001) Construction of a mini-intein fusion system to allow both direct monitoring of soluble protein expression and rapid purification of target proteins. Gene 275, 241-252.
    • (2001) Gene , vol.275 , pp. 241-252
    • Zhang, A.1    Gonzalez, S.M.2    Cantor, E.J.3    Chong, S.4
  • 37
    • 0029834656 scopus 로고    scopus 로고
    • Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system
    • Chong S, Shao Y, Paulus H, Benner J, Perler FB, &, Xu MQ, (1996) Protein splicing involving the Saccharomyces cerevisiae VMA intein. The steps in the splicing pathway, side reactions leading to protein cleavage, and establishment of an in vitro splicing system. J Biol Chem 271, 22159-22168.
    • (1996) J Biol Chem , vol.271 , pp. 22159-22168
    • Chong, S.1    Shao, Y.2    Paulus, H.3    Benner, J.4    Perler, F.B.5    Xu, M.Q.6
  • 38
    • 13244292480 scopus 로고    scopus 로고
    • Kinetic analysis of the individual steps of protein splicing for the Pyrococcus abyssi PolII intein
    • Mills KV, Dorval DM, &, Lewandowski KT, (2005) Kinetic analysis of the individual steps of protein splicing for the Pyrococcus abyssi PolII intein. J Biol Chem 280, 2714-2720.
    • (2005) J Biol Chem , vol.280 , pp. 2714-2720
    • Mills, K.V.1    Dorval, D.M.2    Lewandowski, K.T.3
  • 39
    • 0036295886 scopus 로고    scopus 로고
    • Protein-splicing reaction via a thiazolidine intermediate: Crystal structure of the VMA1-derived endonuclease bearing the N and C-terminal propeptides
    • Mizutani R, Nogami S, Kawasaki M, Ohya Y, Anraku Y, &, Satow Y, (2002) Protein-splicing reaction via a thiazolidine intermediate: crystal structure of the VMA1-derived endonuclease bearing the N and C-terminal propeptides. J Mol Biol 316, 919-929.
    • (2002) J Mol Biol , vol.316 , pp. 919-929
    • Mizutani, R.1    Nogami, S.2    Kawasaki, M.3    Ohya, Y.4    Anraku, Y.5    Satow, Y.6
  • 41
    • 0037106450 scopus 로고    scopus 로고
    • High resolution crystal structure of domain i of the Saccharomyces cerevisiae homing endonuclease PI-SceI
    • Werner E, Wende W, Pingoud A, &, Heinemann U, (2002) High resolution crystal structure of domain I of the Saccharomyces cerevisiae homing endonuclease PI-SceI. Nucleic Acids Res 30, 3962-3971.
    • (2002) Nucleic Acids Res , vol.30 , pp. 3962-3971
    • Werner, E.1    Wende, W.2    Pingoud, A.3    Heinemann, U.4
  • 42
    • 33847071304 scopus 로고    scopus 로고
    • Crystallographic and mutational studies of Mycobacterium tuberculosis recA mini-inteins suggest a pivotal role for a highly conserved aspartate residue
    • Van Roey P, Pereira B, Li Z, Hiraga K, Belfort M, &, Derbyshire V, (2007) Crystallographic and mutational studies of Mycobacterium tuberculosis recA mini-inteins suggest a pivotal role for a highly conserved aspartate residue. J Mol Biol 367, 162-173.
    • (2007) J Mol Biol , vol.367 , pp. 162-173
    • Van Roey, P.1    Pereira, B.2    Li, Z.3    Hiraga, K.4    Belfort, M.5    Derbyshire, V.6
  • 44
    • 2342507637 scopus 로고    scopus 로고
    • Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction
    • Romanelli A, Shekhtman A, Cowburn D, &, Muir TW, (2004) Semisynthesis of a segmental isotopically labeled protein splicing precursor: NMR evidence for an unusual peptide bond at the N-extein-intein junction. Proc Natl Acad Sci USA 101, 6397-6402.
    • (2004) Proc Natl Acad Sci USA , vol.101 , pp. 6397-6402
    • Romanelli, A.1    Shekhtman, A.2    Cowburn, D.3    Muir, T.W.4
  • 45
    • 8844259791 scopus 로고    scopus 로고
    • Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo
    • Tan LP, Lue RY, Chen GY, &, Yao SQ, (2004) Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo. Bioorg Med Chem Lett 14, 6067-6070.
    • (2004) Bioorg Med Chem Lett , vol.14 , pp. 6067-6070
    • Tan, L.P.1    Lue, R.Y.2    Chen, G.Y.3    Yao, S.Q.4
  • 46
    • 77950586384 scopus 로고    scopus 로고
    • Intein-mediated cyclization of bacterial acyl carrier protein stabilizes its folded conformation but does not abolish function
    • Volkmann G, Murphy PW, Rowland EE, Cronan JE Jr, Liu XQ, Blouin C, &, Byers DM, (2010) Intein-mediated cyclization of bacterial acyl carrier protein stabilizes its folded conformation but does not abolish function. J Biol Chem 285, 8605-8614.
    • (2010) J Biol Chem , vol.285 , pp. 8605-8614
    • Volkmann, G.1    Murphy, P.W.2    Rowland, E.E.3    Cronan, Jr.J.E.4    Liu, X.Q.5    Blouin, C.6    Byers, D.M.7
  • 49
    • 0029019782 scopus 로고
    • An arabinose-inducible expression vector, pAR3, compatible with ColE1-derived plasmids
    • Perez-Perez J, &, Gutierrez J, (1995) An arabinose-inducible expression vector, pAR3, compatible with ColE1-derived plasmids. Gene 158, 141-142.
    • (1995) Gene , vol.158 , pp. 141-142
    • Perez-Perez, J.1    Gutierrez, J.2
  • 50
    • 70350502592 scopus 로고    scopus 로고
    • Controllable protein cleavages through intein fragment complementation
    • Volkmann G, Sun W, &, Liu XQ, (2009) Controllable protein cleavages through intein fragment complementation. Protein Sci 18, 2393-2402.
    • (2009) Protein Sci , vol.18 , pp. 2393-2402
    • Volkmann, G.1    Sun, W.2    Liu, X.Q.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.