Intein-mediated cyclization of bacterial acyl carrier protein stabilizes its folded conformation but does not abolish function

Journal of Biological Chemistry

Volumn 285, Issue 12, 2010, Pages 8605-8614

  Volkmann, Gerrit ^a   Murphy, Peter W ^a   Rowland, Elden E ^a   Cronan Jr , John E ^b   Liu, Xiang Qin ^a   Blouin, Christian ^a   Byers, David M ^a  

^a DALHOUSIE UNIVERSITY   (Canada)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ACYL CARRIER PROTEINS; ACYL CHAIN; ACYL DONORS; ACYL GROUP; ACYL-ACP; BIOLOGICAL ACTIVITIES; CIRCULAR DICHROISM; COMPLEMENTATION; FOLDED CONFORMATION; GEL ELECTROPHORESIS; HELICAL CONFORMATION; HELIX BUNDLE; HYDROPHOBIC POCKETS; IN-VIVO; MOLECULAR DYNAMICS SIMULATIONS; NULL MUTANT; POLYPEPTIDE CHAIN; WILD-TYPE PROTEINS;

BACTERIOLOGY; CYCLIZATION; DICHROISM; ELECTROPHORESIS; ESCHERICHIA COLI; FATTY ACIDS; MASS SPECTROMETRY; MOLECULAR DYNAMICS; PHOSPHOLIPIDS;

PROTEINS;

ACYL CARRIER PROTEIN; INTEIN; PHOSPHOLIPID;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; CONTROLLED STUDY; CYCLIZATION; ESCHERICHIA COLI; FLUORESCENCE; GEL ELECTROPHORESIS; IN VIVO STUDY; MASS SPECTROMETRY; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN CONFORMATION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN STRUCTURE; VIBRIO HARVEYI; CHEMICAL STRUCTURE; CHEMISTRY; CONFORMATION; GENETIC COMPLEMENTATION; METABOLISM; METHODOLOGY; MUTATION; PROTEIN DENATURATION; PROTEIN SECONDARY STRUCTURE; TANDEM MASS SPECTROMETRY; VIBRIO;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; VIBRIO HARVEYI;

ACYL CARRIER PROTEIN; CIRCULAR DICHROISM; ESCHERICHIA COLI; GENETIC COMPLEMENTATION TEST; INTEINS; MODELS, MOLECULAR; MOLECULAR CONFORMATION; MUTATION; PHOSPHOLIPIDS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; TANDEM MASS SPECTROMETRY; VIBRIO;

EID: 77950586384     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.060863     Document Type: Article

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