Conditional protein splicing: A new tool to control protein structure and function in vitro and in vivo

Journal of the American Chemical Society

Volumn 125, Issue 35, 2003, Pages 10561-10569

  Mootz, Henning D ^a   Blum, Elyse S ^a   Tyszkiewicz, Amy B ^a   Muir, Tom W ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN SPLICING;

CELLS; POLYPEPTIDES;

PROTEINS;

ASCOMYCIN; MALTOSE BINDING PROTEIN; RAPAMYCIN;

ARTICLE; CATALYSIS; COMPETITIVE INHIBITION; DNA FLANKING REGION; GENE SEQUENCE; IMMUNOPRECIPITATION; PROTEIN DOMAIN; PROTEIN MODIFICATION; PROTEIN PROCESSING; PROTEIN STRUCTURE; PROTEIN TARGETING; SIGNAL TRANSDUCTION; STRUCTURE ACTIVITY RELATION;

ANIMALS; BLOTTING, WESTERN; CARRIER PROTEINS; COS CELLS; DIMERIZATION; HELA CELLS; HISTIDINE; HUMANS; IMMUNOPHILINS; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PROMOTER REGIONS (GENETICS); PROTEIN SPLICING; PROTEIN STRUCTURE, TERTIARY; SIROLIMUS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STRUCTURE-ACTIVITY RELATIONSHIP; TACROLIMUS; TACROLIMUS BINDING PROTEINS; TRANSFECTION; ZINC;

EID: 0041854719     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja0362813     Document Type: Article

References (42)

1. Shogren-Knaak, M. A.; Alaimo, P. J.; Shokat, K. M. Annu Rev. Cell Dev. Biol. 2001, 17, 405-433.
2. Schreiber, S. L. Bioorg. Med. Chem. 1998, 6, 1127-1152.
3. Mayer, T. U.; Kapoor, T. M.; Haggarty, S. J.; King, R. W.; Schreiber, S. L.; Mitchison, T. J. Science 1999, 286, 971-974.
4. Kuruvilla, F. G.; Shamji, A. F.; Sternson, S. M.; Hergenrother, P. J.; Schreiber, S. L. Nature 2002, 416, 653-657.
5. Liberles, S. D.; Diver, S. T.; Austin, D. J.; Schreiber, S. L. Proc. Natl. Acad. Sci. U.S.A. 1997, 94, 7825-7830.
6. Clemons, P. A.; Gladstone, B. G.; Seth, A.; Chao, E. D.; Foley, M. A.; Schreiber, S. L. Chem. Biol. 2002, 9, 49-61.
7. Guo, Z.; Zhou, D.; Schultz, P. G. Science 2000, 288, 2042-2045.
8. Bishop, A. C.; Ubersax, J. A.; Petsch, D. T.; Matheos, D. P.; Gray, N. S.; Blethrow, J.; Shimizu, E.; Tsien, J. Z.; Schultz, P. G.; Rose, M. D.; Wood, J. L.; Morgan, D. O.; Shokat. K. M. Nature 2000, 407, 395-401.
9. Lin, Q.; Jiang, F.; Schultz, P. G.; Gray, N. S. J. Am. Chem. Soc. 2001, 123, 11608-11613.
10. Lin, Q.; Barbas, C. F., III; Schultz, P. G. J. Am. Chem. Soc. 2003, 125, 612-613.
11. Kapoor, T. M.; Mitchison, T. J. Proc. Natl. Acad. Sci. U.S.A. 1999, 96, 9106-9111.
12. The abbreviations used in this paper are: CPS, conditional protein splicing; FKBP, FK506 binding protein; FRB, FKBP-rapamycin-binding; MALDI, matrix-assisted laser desorption ionization; MBP, maltose-binding protein; PAGE, polyacrylamide gel electrophoresis: PBS, phosphate-buffered saline; SDS, sodium dodecyl sulfate; VMA, vacuolar ATPase subunit, Standard single and triple letter amino acid codes were used.
13. Mootz, H. D.; Muir, T. W. J. Am. Chem. Soc. 2002, 124, 9044-9045.
14. Giriat, I.; Muir, T. W.; Perler, F. B. Genet. Eng. (NY) 2001, 23, 171-199.
15. Wu, H.; Hu, Z.; Liu, X. Q. Proc. Natl. Acad. Sci. U.S.A. 1998, 95, 9226-9231.
16. Choi, J.; Chen, J.; Schreiber, S. L.; Clardy, J. Science 1996, 273, 239-242.
17. Muir, T. W. Annu. Rev. Biochem. 2003, 42, 249-289.
18. Ho, S. N.; Biggar, S. R.; Spencer, D. M.; Schreiber, S. L.; Crabtree, G. R. Nature 1996, 382, 822-826.
19. Rivera, V. M.; Clackson, T.; Natesan, S.; Pollock, R.; Amara, J. F.; Keenan, T.; Magari, S. R.; Phillips, T.; Courage, N. L.; Cerasoli, F., Jr.; Holt, D. A.; Gilman, M. Nat. Med. 1996, 2, 1028-1032.
20. Sun, L.; Ghosh, I.; Paulus, H.; Xu, M. Q. Appl. Environ. Microbial. 2001, 67, 1025-1029.
21. Ozawa, T.; Nogami, S.; Sato, M.; Ohya, Y.; Umezawa, Y. Anal. Chem. 2000, 72, 5151-5157.
22. Ozawa, T.; Takeuchi, T. M.; Kaihara, A.; Sato, M.; Umezawa, Y. Anal. Chem. 2001, 73, 5866-5874.
23. Ozawa, T.; Kaihara, A.; Sato, M.; Tachihara, K.; Umezawa, Y. Anal. Chem. 2001, 73, 2516-2521.
24. Ozawa, T.; Sako, Y.; Sato, M.; Kitamura, T.; Umezawa, Y. Nat. Biotechnol. 2003, 21, 287-293.
25. Yang, J.; Fox, G. C., Jr.; Henry-Smith, T. V. Proc. Natl. Acad. Sci. U.S.A. 2003, 100, 3513-3518.
26. Chin, H. G.; Kim, G. D.; Marin, I.; Mersha, F.; Evans, T. C., Jr.; Chen, L.; Xu, M. Q.; Pradhan, S. Proc. Natl. Acad. Sci. U.S.A. 2003, 100, 4510-4515.
27. Giriat, I.; Muir, T. W. J. Am. Chem. Soc. 2003, 125, 7180-7181.
28. Wu, H.; Xu, M. Q.; Liu, X. Q. Biochim. Biophys. Acta. 1998, 1387, 422-432.
29. Shingledecker, K.; Jiang, S. Q.; Paulus, H. Gene 1998, 207, 187-195.
30. Pollock, R.; Giel, M.; Linher, K.; Clackson, T. Nat. Biotechnol. 2002, 20, 729-733.
31. Gossen, M.; Bujard, H. Proc. Natl. Acad. Sci. U.S.A. 1992, 89, 5547-5551.
32. Kawai, M.; Lane, B. C.; Hsieh, G. C.; Mollison, K. W.; Carter, G. W.; Luly, J. R. FEBS Lett. 1993, 316, 107-113.
33. The endonuclease domain comprises amino acids 183-410 of the VMA intein, whereas the intein domain comprises amino acids 1-182 and 411-454 (see refs 34 and 35). The modularity of the VMA intein was previously demonstrated by deletion (ref 36) of the endonuclease domain and by its replacement with calmodulin and its M13 recognition peptide (ref 21).
34. Duan, X.; Gimble, F. S.; Quiocho, F. A. Cell 1997, 89, 555-564.
35. Grindl, W.; Wende, W.; Pingoud, V.; Pingoud, A. Nucleic Acids Res. 1998, 26, 1857-1862.
36. Chong, S.; Xu, M. Q. J. Biol. Chem. 1997, 272, 15587-15590.
37. Poland, B. W.; Xu, M. Q.; Quiocho, F. A. J. Biol. Chem. 2000, 275, 16408-16413.
38. Mills, K. V.; Paulus, H. J. Biol. Chem. 2001, 276, 10832-10838.
39. Ghosh, I.; Sun, L.; Xu, M. Q. J. Biol. Chem. 2001, 276, 24051-24058.
40. Nichols, N. M.; Benner, J. S.; Martin. D. D.; Evans, T. C., Jr. Biochemistry 2003, 42, 5301-5311.
41. To calculate protein concentrations of construct 2b in vivo, extracts of HEK293 cells expressing 2b were immunoblotted against the anti-MBP antibody along with the purified protein 2b of known concentration. Quantification of the protein bands was performed by densitometric analysis, which allowed an estimate of the protein concentration in the extract to be determined, assuming that each cell has as volume of 1 pL.
42. Chong, S.; Williams, K. S.; Wotkowicz, C.; Xu, M. Q. J. Biol. Chem. 1998, 273, 10567-10577.