Green fluorescent protein (GFP) leakage from microbial biosensors provides useful information for the evaluation of the scale-down effect

Biotechnology Journal

Volumn 6, Issue 8, 2011, Pages 968-978

  Delvigne, Frank ^a   Brognaux, Alison ^a   Francis, Frédéric ^a   Twizere, Jean Claude ^a   Gorret, Nathalie ^b   Sorensen, Soren J ^c   Thonart, Philippe ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b INSA   (France)

^c UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Bioreactor mixing; Microbial stress; Protein leakage; Scale down reactor; Secretome

Indexed keywords

CELL VIABILITY; DILUTION RATE; EXTRACELLULAR MEDIUM; FED BATCHES; GREEN FLUORESCENT PROTEIN; HYDRODYNAMIC MODEL; IN-PROCESS; MEMBRANE PERMEABILITY; MICROBIAL BIOSENSOR; MICROBIAL STRESS; MICROBIAL VIABILITY; PROTEIN LEAKAGE; SCALE-DOWN; SECRETOME; SINGLE-CELL LEVEL; WHOLE CELL;

CELL CULTURE; COMPUTER SIMULATION; FLUORESCENCE; GLUCOSE; PROTEINS; STOCHASTIC MODELS;

BIOSENSORS;

GLUCOSE; GREEN FLUORESCENT PROTEIN;

ARTICLE; BIOREACTOR; BIOSENSOR; CELL VIABILITY; ENZYME SUBSTRATE; FED BATCH CULTURE; HYDRODYNAMICS; MEMBRANE PERMEABILITY; NONHUMAN; PRIORITY JOURNAL; PROCESS DEVELOPMENT; STOCHASTIC MODEL;

BIOREACTORS; BIOSENSING TECHNIQUES; CELL MEMBRANE PERMEABILITY; CULTURE MEDIA; ESCHERICHIA COLI; FERMENTATION; GLUCOSE; GREEN FLUORESCENT PROTEINS; HYDRODYNAMICS; INDUSTRIAL MICROBIOLOGY; MODELS, CHEMICAL; SINGLE-CELL ANALYSIS; STOCHASTIC PROCESSES;

EID: 79961175327     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201000410     Document Type: Article

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