Characterization of the response of GFP microbial biosensors sensitive to substrate limitation in scale-down bioreactors

Biochemical Engineering Journal

Volumn 55, Issue 2, 2011, Pages 131-139

  Delvigne, Frank ^a   brognaux, Alison ^a   Gorret, Nathalie ^b   Neubauer, Peter ^c   Delafosse, Angélique ^a,d   Collignon, Marie Laure ^a,d   Toye, Dominique ^a   Crine, Michel ^a   Boxus, Mathieu ^a,d   Thonart, Philippe ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b INSA   (France)

^c TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^d FNRS   (Belgium)

Author keywords

Bioreactor mixing; Microbial stress; Protein leakage

Indexed keywords

CARBON LIMITATION; CULTIVATION CONDITIONS; EXTRACELLULAR MEDIUM; FED BATCHES; FED-BATCH CULTURES; FLUORESCENCE INTENSITIES; FLUORESCENCE LEVEL; GFP EXPRESSION; GREEN FLUORESCENT PROTEIN; MICROBIAL BIOSENSOR; MICROBIAL CELLS; MICROBIAL STRESS; NORMAL MODES; OPERATING CONDITION; PROPIDIUM IODIDE; PROTEIN LEAKAGE; SCALE-DOWN; SINGLE-CELL LEVEL; STRESS RESPONSE; SUBSTRATE LIMITATION; SUBSTRATE PROFILE;

BATCH CELL CULTURE; BIOCONVERSION; BIOREACTORS; BIOSENSORS; CULTIVATION; CURRICULA; DROPS; ENZYME ACTIVITY; FLOW CYTOMETRY; FLUORESCENCE;

SUBSTRATES;

GREEN FLUORESCENT PROTEIN; PROPIDIUM IODIDE;

ARTICLE; BIOREACTOR; CELL CULTURE; FED BATCH CULTURE; FLOW CYTOMETRY; HYDRODYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION;

EID: 79956037572     PISSN: 1369703X     EISSN: 1369703X     Source Type: Journal    
DOI: 10.1016/j.bej.2011.03.016     Document Type: Article

References (36)

1. Neubauer P., Junne S. Scale-down simulators for metabolic analysis of large-scale bioprocesses. Current Opinion in Biotechnology 2010, 21:114-121.
2. Enfors S.O., Jahic M., Rozkov A., Xu B., Hecker M., Jürgen B., Krüger E., Schweder T., Hamer G., O'Beirne D., Noisommit-Rizzi N., Reuss M., Boone L., Hewitt C., McFarlane C., Nienow A., Kovacs T., Trägardh C., Fuchs L., Revstedt J., Friberg P.C., Hjertager B., Blomsten G., Skogman H., Hjort S., Hoeks F., Lin H.Y., Neubauer P., van der Lans R., Luyben K., Vrabel P., Manelius A. Physiological responses to mixing in large scale bioreactors. Journal of Biotechnology 2001, 85:175-185.
3. Lee J., Lee S.Y., Park S., Middelberg A.P.J. Control of fed-batch fermentations. Biotechnology Advances 1999, 17:29-48.
4. Hewitt C.J., Nienow A.W. The scale-up of microbial batch and fed-batch fermentation processes. Advances in Applied Microbiology 2007, 62:105-135.
5. Delvigne F., Boxus M., Ingels S., Thonart P. Bioreactor mixing efficiency modulates the activity of a prpoS::GFP reporter gene in E. coli. Microbial Cell Factories 2009, 8:15.
6. Delvigne F., Ingels S., Thonart P. Evaluation of a set of E. coli reporter strains as physiological tracer for estimating bioreactor hydrodynamic efficiency. Process Biochemistry 2010, 45(11):1769-1778.
7. Mandel M.J., Silhavy T.J. Starvation for different nutrients in Escherichia coli results in differential modulation of RpoS level and stability. Journal of Bacteriology 2005, 187(2):434-442.
8. Notley-McRobb L., King T., Ferenci T. rpoS mutations and loss of general stress resistance in Escherichia coli populations as a consequence of conflict between competing stress responses. Journal of Bacteriology 2002, 184(3):806-811.
9. Hengge-Aronis R. Recent insigths into the general stress response regulatory network in E. coli. Journal of Molecular Biology and Biotechnology 2002, 4(3):341-346.
10. Storz G., Hengge-Aronis E. Bacterial Stress Response 2000, American Society for Microbiology.
11. Ma H.W., Buer J., Zeng A.P. Hierarchical structure and modules in the Escherichia coli transcriptional regulatory network revealed by a new top-down approach. BMC Bioinformatics 2004, 5:199.
12. Marschall C., Hengge-Aronis R. Regulatory characteristics and pormoter analysis of csiE, a stationary phase-inducible gene under the control of sigma S and the cAMP-CRP complex in Escherichia coli. Molecular Microbiology 1995, 18(1):175-184.
13. Zhang Q., Andersen M.E., Conolly R.B. Binary gene induction and protein expression in individual cells. Theoretical Biology and Medical Modelling 2006, 3:18.
14. Zaslaver A., Bren A., Ronen M., Itzkovitz S., Kikoin I., Shavit S., Liebermeister W., Surette M.G., Alon U. A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nature Methods 2006, 3(8):623-628.
15. Lara A.R., Galindo E., Ramirez O.T., Palomares L.A. Living with heterogeneities in bioreactors - understanding the effects of environmental gradients on cells. Molecular Biotechnology 2006, 34:355-381.
16. Delvigne F., Destain J., Thonart P. A methodology for the design of scale-down bioreactors by the use of mixing and circulation stochastic models. Biochemical Engineering Journal 2006, 28(3):256-268.
17. John G.T., Klimant I., Wittmann C., Heinzle E. Integrated optical sensing of dissolved oxygen in microtiter plates: a novel tool for microbial cultivation. Biotechnology and Bioengineering 2003, 81(7):830-836.
18. Cormack B.P., Valdivia R.H., Falkow R. FACS-optimized mutants of the green fluorescent protein (GFP). Gene 1996, 173:33-38.
19. Galbraith D.W., Anderson M.T., Herzenberg L.A. Flow cytometry analysis and FACS sorting of cells based on GFP accumulation. Methods in Cell Biology 1999, 58:315-341.
20. Asraf-Snir M., Gitis V. Tracer studies involving fluorescent-dyed microorganisms - a new method for determination of residence time in chlorination reactors. Chemical Engineering Journal 2011, 166:579-585.
21. Delvigne F., Lejeune A., Destain J., Thonart P. Stochastic models to study the impact of mixing on a fed-batch culture of Saccharomyces cerevisiae. Biotechnology Progress 2006, 22:259-269.
22. Moe D., GArbasch C., Kirkeby S. The protein effect on determination of DNA with Heochst 33258. Journal of Biochemical and Biophysical Methods 1994, 28:263-276.
23. Tsien R.Y. The green fluorescent protein. Annual Review in Biochemistry 1998, 67:509-544.
24. Müller S., Harms H., Bley T. Origin and analysis of microbial population heterogeneity in bioprocesses. Current Opinion in Biotechnology 2010, 21:100-113.
25. Pugsley A.P. The complete general secretory pathway in Gram-negative bacteria. Microbiological Reviews 1993, 57(1):50-108.
26. Liu S., Bugos R.C., Dharmasiri N., Su W.W. Green fluorescent protein as a secretory reporter and a tool for process optimization in transgenic plant cell cultures. Journal of Biotechnology 2001, 87:1-16.
27. Lowder M., Unge A., Maraha N., Jansson J.K., Swiggett J., Oliver J.D. Effect of starvation and the viable but nonculturable state on green fluorescent protein (GFP) fluorescence in GFP-tagged pseudomonas fluorescens A506. Applied and Environmental Microbiology 2000, 66(8):3160-3165.
28. Klotz B., Manas P., Mackey B.M. The relationship between membrane damage, release of protein and loss of viability in Escherichia coli exposed to high hydrostatic pressure. International Journal of Food Microbiology 2010, 137:214-220.
29. Liu X., Ferenci T. Regulation of porin-mediated outer membrane permeability by nutrient limitation in Escherichia coli. Journal of Bacteriology 1998, 180(15):3917-3922.
30. Shokri A., Sanden A.M., Larsson G. Growth rate dependent changes in Escherichia coli membrane structure and protein leakage. Applied Microbiology and Biotechnology 2002, 58:386-392.
31. Fischer A.C., DeLisa M.P. Laboratory evolution of fast-folding green fluorescent protein using secretory pathway quality control. PLoS One 2008, 3(6):e2351.
32. Xia X.X., Han M.J., Lee S.Y., Yoo J.S. Comparison of the extracellular proteomes of Eschercihia coli B and K-12 strains during high cell density cultivation. Proteomics 2008, 8:2089-2103.
33. Castan A., Heidrich J., Enfors S.O. The use of flow cytometry to detect nucleic acids attached to the surface of Escherichia coli in high cell density fed-batch processes. Biotechnology Letters 2002, 24:219-224.
34. Hewitt C.J., Nebe-Von Caron G., Axelsson B., Mc Farlane C.M., Nienow A.W. Studies related to the scale-up of high-cell-density E. coli fed-batch fermentations using multiparameter flow cytometry: effect of a changing microenvironment with respect to glucose and dissolved oxygen concentration. Biotechnology and Bioengineering 2000, 70(4):381-390.
35. Hewitt C.J., Nebe-Von Caron G., Nienow A.W., Mc Farlane C.M. The use of multi-parameter flow cytomerty to compare the physiological response of Escerichia coli W3110 to glucose limitation during batch, fed-batch and continuous culture cultivations. Journal of Biotechnology 1999, 75:251-264.
36. Berney M, Weilenmann H.U., Ihssen J., Bassin C., Egli T. Specific growth rate determines the sensitivity of Escherichia coli to thermal, UV and solar disinfection. Applied and Environmental Microbiology 2006, 72(4):2586-2593.