Protein secretion in Escherichia coli K-12: Dead or alive?

Cellular and Molecular Life Sciences

Volumn 54, Issue 4, 1998, Pages 347-352

  Pugsley, A P ^a   Francetic O ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

General secretory pathway; Outer membrane; Pili; Protein secretion; Secreton

Indexed keywords

BACTERIAL PROTEIN; POLYMER;

BIOSENSOR; CONFERENCE PAPER; CULTURE MEDIUM; ESCHERICHIA COLI K 12; GENETIC COMPLEMENTATION; GRAM NEGATIVE BACTERIUM; NONHUMAN; PATHOGENICITY; PROTEIN SECRETION;

BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL PROTEINS; CHROMOSOMES, BACTERIAL; ESCHERICHIA COLI; FIMBRIAE, BACTERIAL; GENES, BACTERIAL; GRAM-NEGATIVE BACTERIA;

BACTERIA (MICROORGANISMS); ESCHERICHIA; ESCHERICHIA COLI K12; NEGIBACTERIA;

EID: 0031922530     PISSN: 1420682X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s000180050162     Document Type: Conference Paper

References (51)

1. Wandersman C. (1996) Secretion across the bacterial outer membrane. In: Escherichia coli and Salmonella. Cellular and Molecular Biology, pp. 955-966, Neidhardt F. C., Curtiss III R., Ingraham J. L., Lin E. C. C., Low K. B., Magasanik B. et al. (eds), ASM Press, Washington DC
2. Pugsley A. P. (1993) The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57: 50-108
3. Sauvonnet N. and Pugsley A. P. (1996) Identification of two regions of Klebsiella oxytoca pullulanase that together are capable of promoting β-lactamase secretion by the general secretory pathway. Mol. Microbiol. 22: 1-7
4. Hardie K. R., Lory S. and Pugsley A. P. (1996) Insertion of an outer membrane protein in Escherichia coli requires a chaperone-like protein. EMBO J. 15: 978-988
5. Hardie K. R., Seydel A., Guilvout I. and Pugsley A. P. (1996) The secretin-specific, chaperone-like protein of the general secretory pathway: separation of proteolytic protection and piloting functions. Mol. Microbiol. 22: 967-976
6. Pugsley A. P. (1993) Processing and methylation of PulG, a pilin-like component of the general secretory pathway of Klebsiella oxytoca. Mol. Microbol. 9: 295-308
7. Pugsley A. P. and Dupuy B. (1992) An enzyme with type IV prepilin peptidase activity is required to process a component of the general extracellular protein secretion pathway of Klebsiella oxytoca. Mol. Microbiol. 6: 751-760
8. Possot O. and Pugsley A. P. (1994) Molecular characterization of PulE, a protein required for pullulanase secretion. Mol. Microbiol. 12: 287-299
9. Possot O., Letellier L. and Pugsley A. P. (1997) Energy requirement for pullulanase secretion by the main terminal branch of the general secretory pathway. Mol. Microbiol. 24: 457-464
10. Possot O. and Pugsley A. (1997) The conserved tetracysteine motif in the general secretory pathway component PulE is required for efficient pullulanase secretion. Gene 192: 45-50
11. Lindeberg M., Salmond G. P. C. and Collmer A. (1996) Complementation of deletion mutations in a cloned functional cluster of Erwinia chrysanthemi out genes with Erwinia carotovora out homologs reveals OutC and OutD as candidate gatekeepers of species-specific secretion of proteins via the Type II pathway. Mol. Microbiol. 20: 175-190
12. Pugsley A. P. (1996) Multimers of the precursor of a type IV pilin-like component of the general secretory pathway are unrelated to pili. Mol. Microbiol. 20: 1235-1245
13. d'Enfert C., Reyss I., Wandersman C. and Pugsley A. P. (1989) Protein secretion by Gram-negative bacteria: characterization of two membrane proteins required for pullulanase secretion by Escherichia coli K-12. J. Biol. Chem. 264: 17462-17468
14. Pugsley A. P. and Reyss I. (1990) Five genes at the 3′ end of the Klebsiella pneumonia? pulC operon are required for pullulanase secretion. Mol. Microbiol. 4: 365-379
15. Reyss I. and Pugsley A. P. (1990) Five additional genes in the pulC-O operon of the Gram-negative bacterium Klebsiella oxytoca UNF5023 that are required for pullulanase secretion. Mol. Gen. Genet. 222: 176-184
16. Plunkett G. (1995) Escherichia coli K-12 chromosomal region from 67.4 to 76.0 minutes. GenBank accession number U18997
17. Francetic O. and Pugsley A. P. (1996) The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins. J. Bacteriol. 178: 3544-3549
18. Fujita N., Mori H., Yura T. and Ishihama A. (1994) Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917 - 193,643 bp) region. Nucleic Acids Res. 22: 1637-1639
19. Howard S. P., Meiklejohn H. C., Shivak D. and Jahagirdar R. (1996) A TonB-like protein and a novel membrane protein containing an ATP-binding cassette function together in exotoxin secretion. Mol. Microbiol. 22: 595-604
20. Francetič O., Lory S. and Pugsley A. P. (1998) A second prepilin peptidase gene in Escherichia coli K-12. Mol. Microbiol. 27: 763-775
21. Nunn D. and Lory S. (1992) Components of the protein excretion apparatus of Pseudomonas aeruginosa are processed by the type IV prepilin peptidase. Proc. Natl Acad. Sci. USA 89: 47-51
22. Nunn D. N. and Lory S. (1993) Cleavage, methylation and localization of the Pseudomonas aeruginosa export proteins XcpT, -U, -V and -W. J. Bacteriol. 175: 4375-4382
23. Bally M., Filloux A., Akrim M., Ball G., Lazdunski A. and Tommassen J. (1992) Protein secretion in Pseudomonas aeruginosa: characterization of seven xcp genes and processing of secretory apparatus components by prepilin peptidase. Mol. Microbiol. 6: 1121-1131
24. Nunn D. and Lory S. (1991) Product of the Pseudomonas aeruginosa gene pilD is a prepilin leader peptidase. Proc. Natl. Acad. Sci. USA 88: 3281-3285
25. Pugsley A. P. and Possot O. (1993) The general secretory pathway of Klebsiella oxytoca: no evidence for relocalization or assembly of pilin-like PulG protein into a multiprotein complex. Mol. Microbiol. 10: 665-674
26. Whitchurch C. B. and Mattick J. S. (1994) Escherichia coli contains a set of genes homologous to those involved in protein secretion. DNA uptake and the assembly of type-4 fimbriae in other bacteria. Gene 150: 9-15
27. Nunn D., Bergman S. and Lory S. (1990) Products of three accessory genes, pilB, pilC and pilD, are required for biogenesis of Pseudomonas aeruginosa pili. J. Bacteriol. 172: 2911-2919
28. Tonjum R., Freitag N. E., Namork E. and Koomey M. (1995) Identification and characterization of pilG, a highly conserved pilus-assembly gene in pathogenic Neisseria. Mol. Microbiol. 16: 451-464
29. Lauer P., Albertson N. H. and Koomey M. (1993) Conservation of genes encoding components of a type IV pilus assembly two-step protein export pathway in Neisseria gonorrhoeae. Mol. Microbiol. 8: 357-368
30. Freitag N. E., Seifert H. S. and Koomey M. (1995) Characterization of the pilF-pilD pilus-assembly locus of Neisseria gonorrhoeae. Mol. Microbiol. 16: 575-586
31. Giron J. A., Levine M. M. and Kaper J. B. (1994) Longus: a long pilus ultrastructure produced by human enteropathogenic Escherichia coli. Mol. Microbiol. 12: 71-82
32. Giron J. A., Suk Hue Ho A. and Schoolnik G. K. (1991) An inducible bundle-forming pilus of enteropathogenic Escherichia coli. Science 254: 710-713
33. Donnenberg M. S., Giron J. A., Nataro J. P. and Kaper J. B. (1992) A plasmid-encoded type IV fimbrial gene of enteropathogenic Escherichia coli associated with localized adherence. Mol. Microbiol. 6: 6427-3437
34. Kim S.-R. and Komano T. (1997) The plasmid R64 thin pilus identified as type IV pilus. J. Bacteriol. 179: 3594-3603
35. del Castillo F., Leal S. C., Moreno F. and del Castillo I. (1997) The Escherichia coli K-12 sheA gene encodes a 34-kDa secreted haemolysin. Mol. Microbiol. 25: 107-115
36. Lee K. K., Sheth H. B., Wong W. Y., Sherburne, R., Paranchych W., Hodges R. S. et al. (1994) The binding of pseudomonas aeruginosa pili to glycosphingolipids is a tip-associated event involving the C-terminal region of the structural pilin subunit. Mol. Microbiol. 11: 705-713
37. Virji M., Makepeace K., Peak I. R., Ferguson D. J., Jennings M. P. and Moxon E. R. (1995) Ope-and pilus-dependent interactions of meningococci with human endothelial cells: molecular mechanisms and modulation by surface polysaccharides. Mol. Microbiol. 18: 741-754
38. d'Enfert C. and Pugsley A. P. (1989) Klebsiella pneumoniae pulS gene encodes an outer membrane lipoprotein required for pullulanase secretion. J. Bacteriol. 171: 3673-3679
39. d'Enfert C., Ryter A. and Pugsley A. P. (1987) Cloning and expression in Escherichia coli of the klebsiella pneumoniae genes for production, surface localization and secretion of the lipoprotein pullulanase. EMBO J. 6: 3531-3538
40. Condemine G., Dorel C., Hugouvieux-Cotlte-Pattat N. and Robert-Baudouy J. (1992) Some of the out genes involved in the secrelion of pectate lyases in Erwinia chrysanthemi are regulated by kdgR. Mol. Microbiol. 6: 3199-3211
41. Jahagirdar R. and Howard S. P. (1994) Isolation and characterization of a second exe operon required for extracellular protein secretion in Acromonas hydrophila. J. Bacteriol. 176: 6819-6826
42. Jiang B. and Howard S. P. (1992) The Aeromonas hydrophila exeE gene, required both for protein secretion and normal outer membrane biogenesis, is a member of a general secretion pathway. Mol. Microbiol. 6: 1351-1361
43. Akrim M., Bally M., Ball G., Tommassen J., Teerink H., Filloux A. et al. (1993) Xep-niediated protein secretion in Pseudomonas aeruginosa: identification of two additional genes and evidence for regulation of xcp gene expression. Mol. Microbiol. 10: 431-443
44. Andrews S. C., Harrison P. M. and Guest J. R. (1989) Cloning, sequencing and mapping of the bacterioferritin gene (bfr) of Escherichia coli K-12. J. Bacteriol. 171: 3940-3947
45. Bally M., Ball G., Badère A. and Lazdunski A. (1991) Protein secretion in Pseudomonas aeruginosa: the xcpA gene encodes an integral membrane protein homologous to Klebsiella pseudomoniae secretion function PulO. J. Bacteriol. 173: 479-486
46. Dums F., Dow J. M. and Daniels M. J. (1991) Structural characterization of protein secretion genes of the bacterial phytopathogen Xanthomonas campestris pathovar campestris: relatedness to secretion systems of other Gram-negative bacteria. Mol. Gen. Genet. 229: 357-364
47. Howard S. P., Critch J. and Bedi A. (1993) Isolation and analysis of eight exe genes and their involvement in extracellular protein secretion and outer membrane assembly in Aeromonas hydrophila. J. Bacteriol. 175: 6695-6703
48. Hu N.-T., Hung M.-I., Chiou S.-J., Tang F., Chiang D.-C., Huang H.-Y. et al. (1992) Cloning and characterization of a gene required for the secretion of extracellular enzymes across the outer membrane by Xanthomonas campestris. J. Bacteriol. 174: 2679-2687
49. Schmidt H., Henkel B. and Karch H. (1997) A gene cluster related to type II secretion pathway operons of Gram-negative bacteria is located on the large plasmid of enterohemorraghic Escherichia coli O157 strains. FEMS Microbiol. Lett. 148: 265-272
50. Karlyshev A. V. and MacIntyre S. (1995) Cloning and study of the genetic organization of the exe gene cluster of Aeromonas salmonicida. Gene 158: 77-82
51. Tabor S. and Richardson C. C. (1985) A bacteriophage T7 RNA polymerase promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA 82: 1074-1078