Biodosimetry using radiation-induced micronuclei in skin fibroblasts

International Journal of Radiation Biology

Volumn 87, Issue 8, 2011, Pages 824-838

  Kaspler, Pavel ^a   Chen, Rui ^b   Hyrien, Ollivier ^b   Jelveh, Salomeh ^a   Bristow, Robert G ^a,c   Hill, Richard P ^a,c  

^a PRINCESS MARGARET HOSPITAL   (Canada)

^b UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^c UNIVERSITY OF TORONTO   (Canada)

Author keywords

biodosimetry; mice; micronucleus assay; radiation; skin fibroblasts

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL ISOLATION; CONTROLLED STUDY; DOSIMETRY; FEMALE; GAMMA RADIATION; IN VIVO STUDY; MALE; MICRONUCLEUS; MOUSE; NONHUMAN; PRIORITY JOURNAL; RADIATION ACCIDENT; RADIATION DOSE; RADIATION EXPOSURE; RADIATION RESPONSE; SENSITIVITY ANALYSIS; SKIN FIBROBLAST; WHOLE BODY RADIATION;

ANIMALS; BIOLOGICAL ASSAY; FEMALE; FIBROBLASTS; MALE; MICE; MICE, INBRED C3H; MICRONUCLEI, CHROMOSOME-DEFECTIVE; MICRONUCLEUS TESTS; RADIATION DOSAGE; RADIOMETRY; SKIN PHYSIOLOGICAL PHENOMENA; WHOLE-BODY IRRADIATION;

MUS;

EID: 79961048635     PISSN: 09553002     EISSN: 13623095     Source Type: Journal    
DOI: 10.3109/09553002.2011.582927     Document Type: Article

References (75)

1. Abramsson-Zetterberg L, Zetterberg G, Bergqvist M, Grawe J. 2000. Human cytogenetic biomonitoring using flow-cytometric analysis of micronuclei in transferrin-positive immature peripheral blood reticulocytes. Environmental and Molecular Mutagenesis 36:22-31.
2. Angelini S, Kumar R, Carbone F, Maffei F, Forti GC, Violante FS, Lodi V, Curti S, Hemminki K, Hrelia P. 2005. Micronuclei in humans induced by exposure to low level of ionizing radiation: Influence of polymorphisms in DNA repair genes. Mutation Research 570:105-117. (Pubitemid 40179273)
3. Ban S, Cologne JB, Fujita S, Awa AA. 1993. Radiosensitivity of atomic bomb survivors as determined with a micronucleus assay. Radiation Research 134:170-178. (Pubitemid 23157442)
4. Bauchinger M. 1997. Health impacts of large releases of radionuclides. Cytogenetic effects as quantitative indicators of radiation exposure. Ciba Foundation Symposium203:188-199.
5. Bertsche U. 1985. Micronucleus induction in mammalian cell cultures treated with ionizing radiations. Radiation and Environmental Biophysics 24:27-44. (Pubitemid 15195350)
6. Bhogal N, Kaspler P, Jalali F, Hyrien O, Chen R, Hill RP, Bristow RG. 2010. Late residual gamma-H2AX foci in murine skin are dose responsive and predict radiosensitivity in vivo. Radiation Research 173:1-9.
7. Calveley VL, Jelveh S, Langan A,Mahmood J, Yeung IW, Van Dyk J, Hill RP. 2010. Genistein can mitigate the effect of radiation on rat lung tissue. Radiation Research 173:602-611.
8. Chang WP, Hwang BF, Wang D, Wang JD. 1997. Cytogenetic effect of chronic low-dose, low-dose-rate gamma-radiation in residents of irradiated buildings. The Lancet 350:330-333. (Pubitemid 27351116)
9. Chen Y, Tsai Y, Nowak I, Wang N, Hyrien O, Wilkins R, Ferrarotto C, Sun H, Dertinger SD. 2010. Validating highthroughput micronucleus analysis of peripheral reticulocytes for radiation biodosimetry: Benchmark against dicentric and CBMN assays in a mouse model. Health Physics 98:218-227.
10. Cho YH, Kim YJ, An YS, Woo HD, Choi SY, Kang CM, Chung HW. 2009. Micronucleus-centromere assay and DNA repair gene polymorphism in lymphocytes of industrial radiographers. Mutation Research 680:17-24.
11. Clarkson R, Lindsay PE, Ansell S, Wilson G, Jelveh S, Hill RP, Jaffray DA. 2011. Characterization of image quality and imageguidance performance of a preclinical microirradiator. Med Phys 38:845-856.
12. da Cruz AD, McArthur AG, Silva CC, Curado MP, Glickman BW. 1994. Human micronucleus counts are correlated with age, smoking, and cesium-137 dose in the Goiania (Brazil) radiological accident. Mutation Research 313:57-68. (Pubitemid 24247066)
13. Das B, Karuppasamy CV. 2009. Spontaneous frequency of micronuclei among the newborns from high level natural radiation areas of Kerala in the southwest coast of India. International Journal of Radiation Biology 85:272-280.
14. Dertinger SD, Bemis JC, Phonethepswath S, Tsai Y, Nowak I, Hyrien O, Palis J, Chen Y. 2009. Reticulocyte and micronucleated reticulocyte responses to gamma irradiation: Effect of age. Mutation Research 675:77-80.
15. Dileto CL, Travis EL. 1996. Fibroblast radiosensitivity in vitro and lung fibrosis in vivo: Comparison between a fibrosis-prone and fibrosis-resistant mouse strain. Radiation Research 146:61-67. (Pubitemid 26230834)
16. Dolphin GW. 1969. Biological dosimetry with particular reference to chromosome aberration analysis: A review of methods. In: Handling of Radiation Accidents (IAEA Proceedings Series STI/PUB/229). Vienna: International Atomic Energy Agency. pp 215-224.
17. Fenech M. 1993. The cytokinesis-block micronucleus technique: A detailed description of the method and its application to genotoxicity studies in human populations. Mutation Research 285:35-44. (Pubitemid 23029552)
18. Fenech M. 2000. The in vitro micronucleus technique. Mutation Research 455:81-95.
19. Fenech M, Morley AA. 1985. The effect of donor age on spontaneous and induced micronuclei. Mutation Research 148:99-105. (Pubitemid 15151667)
20. Fenech M, Morley AA. 1986. Cytokinesis-block micronucleus method in human lymphocytes: Effect of in vivo ageing and low dose X-irradiation. Mutation Research 161:193-198. (Pubitemid 16099496)
21. Flegal FN, Devantier Y, McNamee JP, Wilkins RC. 2010. Quickscan dicentric chromosome analysis for radiation biodosimetry. Health Physics 98:276-281.
22. Hadjidekova VB, Bulanova M, Bonassi S, Neri M. 2003. Micronucleus frequency is increased in peripheral blood lymphocytes of nuclear power plant workers. Radiation Research 160:684-690. (Pubitemid 37521712)
23. Hill RP, Kaspler P, Griffin AM, O'Sullivan B, Catton C, Alasti H, Abbas A, Heydarian M, Ferguson P, Wunder JS, Bell RS. 2007. Studies of the in vivo radiosensitivity of human skin fibroblasts. Radiotherapy and Oncology 84:75-83. (Pubitemid 47039180)
24. International Atomic Energy Agency (IAEA). 2001. Cytogenetic analysis for radiation dose assessment, a manual. Technical Report Series No. 405. Vienna, Austria: IAEA.
25. Ibrulj S, Krunic-Haveric A, Haveric S, Pojskic N, Hadziselimovic R. 2004. Micronuclei occurrence in population exposed to depleted uranium and control human group in correlation with sex, age and smoking habit. Medicinski Arkhiv 58:335-338.
26. Ilyinskikh NN, Eremich AV, Ivanchuk, II, Ilyinskikh EN. 1997. Micronucleus test of erythrocytes and lymphocytes in the blood of the Altai region residents living near the Semipalatinsk atomic proving ground. Mutation Research 392:223-228. (Pubitemid 27355199)
27. Ilyinskikh NN, Ilyinskikh EN, Ilyinskikh IN. 1998. Micronucleated erythrocytes frequency and radiocesium bioconcentration in pikes (Esox lucius) caught in the Tom River near the nuclear facilities of the Siberian Chemical Complex (Tomsk- 7). Mutation Research 421:197-203. (Pubitemid 28511317)
28. Ilyinskikh NN, Ilyinskikh IN, Porovskiy VA, Natarajan AT, Suskov, II, Smirenniy LN, Ilyinskikh EN. 1999. Biodosimetry results obtained by various cytogenetic methods and electron spin resonance spectrometry among inhabitants of a radionuclide contaminated area around the Siberian chemical plant (Tomsk-7). Mutagenesis 14:473-478. (Pubitemid 29433489)
29. Jen MH, Hwang JJ, Yang JY, Nabyvanets YB, Hsieh WA, Tsai MH, Guo SD, Chang WP. 2002. Micronuclei and nuclear anomalies in urinary exfoliated cells of subjects in radionuclide- contaminated regions. Mutation Research 520:39-46. (Pubitemid 35247571)
30. Joksic G, Spasojevic-Tisma V. 1998. Chromosome analysis of lymphocytes from radiation workers in tritium-applying industry. International Archives of Occupational and Environmental Health 71:213-220. (Pubitemid 28188090)
31. Joseph LJ, Bhartiya US, Raut YS, Kand P, Hawaldar RW, Nair N. 2009. Micronuclei frequency in peripheral blood lymphocytes of thyroid cancer patients after radioiodine therapy and its relationship with metastasis. Mutation Research 675:35-40.
32. Joseph LJ, Patwardhan UN, Samuel AM. 2004. Frequency of micronuclei in peripheral blood lymphocytes from subjects occupationally exposed to low levels of ionizing radiation. Mutation Research 564:83-88. (Pubitemid 39345829)
33. Kanda R, Eguchi-Kasai K, Itsukaichi H, Mori M, Hayata I. 1999. Chemically induced premature chromosome condensation in human fibroblast cell lines: Fundamental study for applications to the biodosimetry of local exposure. Somatic Cell and Molecular Genetics 25:317-325.
34. Kaspler P, Hyrien O, Hill RP. 2010. Dynamics of micronuclei in mouse skin fibroblasts after gamma irradiation. Health Physics 98:228-233.
35. Kaspler P, Pintilie M, Hill RP. 2009. Dynamics of micronuclei in rat skin fibroblasts after X irradiation. Radiation Research 172:106-113.
36. Kerekes J, Novak J, Koteles GJ. 1988. Micronucleus frequency in peripheral lymphocytes for the differential diagnosis of radiation injuries combined with thermal burns. Journal of Burn Care and Rehabilitation 9:275-278.
37. Langan AR, Khan MA, Yeung IW, Van Dyk J, Hill RP. 2006. Partial volume rat lung irradiation: The protective/mitigating effects of Eukarion-189, a superoxide dismutase-catalase mimetic. Radiotherapy and Oncology 79:231-238. (Pubitemid 43884942)
38. Laurent C, Pouget JP, Voisin P. 2005. Modulation of DNA damage by pentoxifylline and alpha-tocopherol in skin fibroblasts exposed to Gamma rays. Radiation Research 164:63-72. (Pubitemid 40885231)
39. Lee TK, O'Brien KF, Naves JL, Christie KI, Arastu HH, Eaves GS, Wiley AL Jr, Karlsson UL, Salehpour MR. 2000. Micronuclei in lymphocytes of prostate cancer patients undergoing radiation therapy. Mutation Research 469:63-70. (Pubitemid 30628675)
40. Lisochkin BG, Kravtsov V, Rybachenko VV. 2004. [Retrospective diagnosis of radiation inhalation lesions of the bronchial mucosa in clean-up personnel after the nuclear accident at the Chernobyl nuclear reactor using the micronucleus test]. Arkhiv Patologii 66:17-19. (Pubitemid 38880747)
41. Loomis DP, Shy CM, Allen JW, Saccomanno G. 1990. Micronuclei in epithelial cells from sputum of uranium workers. Scandinavian Journal of Work, Environment and Health 16:355-362. (Pubitemid 20319963)
42. Maffei F, Angelini S, Forti GC, Lodi V, Violante FS, Mattioli S, Hrelia P. 2002. Micronuclei frequencies in hospital workers occupationally exposed to low levels of ionizing radiation: Influence of smoking status and other factors. Mutagenesis 17:405-409.
43. Mahmood J, Jelveh S, Calveley V, Zaidi A, Doctrow SR, Hill RP. 2011. Mitigation of radiation-induced lung injury by genistein and EUK-207. International Journal of Radiation Biology 87:889-901.
44. Maluf SW, Passos DF, Bacelar A, Speit G, Erdtmann B. 2001. Assessment of DNA damage in lymphocytes of workers exposed to X-radiation using the micronucleus test and the comet assay. Environmental and Molecular Mutagenesis 38:311-315. (Pubitemid 34052166)
45. Martins EP, Fuzzi HT, Kayser C, Alarcon RT, Rocha MG, Chauffaille ML, Andrade LE. 2010. Increased chromosome damage in systemic sclerosis skin fibroblasts. Scandinavian Journal of Rheumatology 39:398-401.
46. Mikhalevich LS, De Zwart FA, Perepetskaya GA, Chebotareva NV, Mikhalevich EA, Tates AD. 2000. Radiation effects in lymphocytes of children living in a Chernobyl contaminated region of Belarus. International Journal of Radiation Biology 76:1377-1385.
47. Muller WU, Dietl S, Wuttke K, Reiners C, Biko J, Demidchik E, Streffer C. 2004. Micronucleus formation in lymphocytes of children from the vicinity of Chernobyl after (131)I therapy. Radiation and Environmental Biophysics 43:7-13. (Pubitemid 38720886)
48. Nachtrab U, Oppitz U, Flentje M, Stopper H. 1998. Radiationinduced micronucleus formation in human skin fibroblasts of patients showing severe and normal tissue damage after radiotherapy. International Journal of Radiation Biology 73:279-287. (Pubitemid 28160914)
49. Neri M, Fucic A, Knudsen LE, Lando C, Merlo F, Bonassi S. 2003. Micronuclei frequency in children exposed to environmental mutagens: A review. Mutation Research 544:243-254. (Pubitemid 37487666)
50. Norman A, Cochran S, Bass D, Roe D. 1984. Effects of age, sex and diagnostic X-rays on chromosome damage. International Journal of Radiation Biology 46:317-321. (Pubitemid 14008920)
51. Paillole N, Voisin P. 1998. Is micronuclei yield variability a problem for overexposure dose assessment to ionizing radiation? Mutation Research 413:47-56. (Pubitemid 28218246)
52. Popova L, Kishkilova D, Hadjidekova VB, Hristova RP, Atanasova P, Hadjidekova VV, Ziya D, Hadjidekov VG. 2007. Micronucleus test in buccal epithelium cells from patients subjected to panoramic radiography. Dentomaxillofacial Radiology 36:168-171. (Pubitemid 46999160)
53. Potten CS. 1986. Biological dosimetry of local radiation accidents of skin: Possible cytological and biochemical methods. British Journal of Radiology Supplement 19:82-85.
54. Pouget JP, Laurent C, Delbos M, Benderitter M, Clairand I, Trompier F, Stephanazzi J, Carsin H, Lambert F, Voisin P, Gourmelon P. 2004. PCC-FISHin skin fibroblasts for local dose assessment: Biodosimetric analysis of a victim of the Georgian radiological accident. Radiation Research 162:365-376. (Pubitemid 39388158)
55. Reus AA, Usta M, van Meeuwen RN, Maas WJ, Robinson SA, Kenny JD, Pruimboom-Brees I, Clements PJ, Lynch AM, Krul CA. 2010. Development and characterization of an in vivo skin photomicronucleus assay in rats. Mutagenesis 25:407-416.
56. Rummelein B, Drieschner O, Ehlert U, Weichenthal M, Breitbart EW, Rudiger HW. 1992. Increased mutagen sensitivity in human cultured fibroblasts with constitutively high micronucleus levels. Cancer Genetics and Cytogenetics 58:186-190.
57. Sari-Minodier I, Orsiere T, Auquier P, Martin F, Botta A. 2007. Cytogenetic monitoring by use of the micronucleus assay among hospital workers exposed to low doses of ionizing radiation. Mutation Research 629:111-121. (Pubitemid 46603084)
58. Senthamizhchelvan S, Pant GS, Rath GK, Julka PK, Nair O. 2009. Biodosimetry using micronucleus assay in acute partial body therapeutic irradiation. Physica Medica 25:82-87.
59. Senthamizhchelvan S, Pant GS, Rath GK, Julka PK, Nair O, Prabhakar R, Malhotra A. 2008. Biological estimation of dose in hemi-body irradiation of cancer patients by cytogenetic analysis. Health Physics 94:112-117.
60. Slonina D, Biesaga B, Urbanski K, Kojs Z. 2007. Low-dose radiation response of primary keratinocytes and fibroblasts from patients with cervix cancer. Radiation Research 167:251-259. (Pubitemid 351292536)
61. Stierum RH, Hageman GJ, van Herwijnen MH, van der Veer MS, Kleinjans JC. 1997. Age-related negative associations between parameters of cytogenetic damage and ex vivo (p/7)-antibenzo( a)pyrene diolepoxide-induced unscheduled DNA synthesis in smoking humans. Cancer Epidemiology, Biomarkers and Prevention 6:943-948. (Pubitemid 27481643)
62. Straume T, Amundson SA, Blakely WF, Burns FJ, Chen A, Dainiak N, Franklin S, Leary JA, Loftus DJ, Morgan WF, Pellmar TC, Stolc V, Turteltaub KW, Vaughan AT, Vijayakumar S, Wyrobek AJ. 2008. NASA Radiation Biomarker Workshop, September 27-28, 2007. Radiation Research 170:393-405.
63. Thierens H, De Ruyck K, Vral A, de Gelder V, Whitehouse CA, Tawn EJ, Boesman I. 2005. Cytogenetic biodosimetry of an accidental exposure of a radiological worker using multiple assays. Radiation Protection Dosimetry 113:408-414. (Pubitemid 40873452)
64. Thierens H, Vral A. 2009. The micronucleus assay in radiation accidents. Annali dell'Istituto Superiore di Sanita 45:260-264.
65. Thierens H, Vral A, De Ridder L. 1996. A cytogenetic study of radiological workers: Effect of age, smoking and radiation burden on the micronucleus frequency. Mutation Research 360:75-82. (Pubitemid 26199673)
66. Thierens H, Vral A, Morthier R, Aousalah B, De Ridder L. 2000. Cytogenetic monitoring of hospital workers occupationally exposed to ionizing radiation using the micronucleus centromere assay. Mutagenesis 15:245-249. (Pubitemid 30312715)
67. Timmermans R, Leonard A. 1984. [Effect of age on the chromosomal radiosensitivity of bone marrow erythrocytes in the mouse]. Compes Rendus des Seances de la Societe de Biologie et de ses Filiales 178:557-561. (Pubitemid 15166355)
68. Touil N, Aka PV, Buchet JP, Thierens H, Kirsch-Volders M. 2002. Assessment of genotoxic effects related to chronic low level exposure to ionizing radiation using biomarkers for DNA damage and repair. Mutagenesis 17:223-232. (Pubitemid 34537752)
69. Vorobtsova IE, Semenov AV. 2006. [Complex cytogenetic characteristic of people suffered from Chernobyl accident]. Radiatsionnaia Biologiia, Radioecologiia 46:140-151.
70. Wones R, Radack K, Martin V, Mandell K, Pinney S, Buncher R. 1995. Do persons living near a uranium processing site have evidence of increased somatic cell gene mutations? A first study. Mutation Research 335:171-184.
71. Yamada Y, Oghiso Y, Enomoto H, Ishigure N. 2002. Induction of micronuclei in a rat alveolar epithelial cell line by alpha particle irradiation. Radiation Protection Dosimetry 99:219-222.
72. Yuan R, Tsaih SW, Petkova SB, Marin de Evsikova C, Xing S, Marion MA, Bogue MA, Mills KD, Peters LL, Bult CJ, Rosen CJ, Sundberg JP, Harrison DE, Churchill GA, Paigen B. 2009. Aging in inbred strains of mice: Study design and interim report on median lifespans and circulating IGF1 levels. Aging Cell 8:277-287.
73. Zakeri F, Assaei RG. 2004. Cytogenetic monitoring of personnel working in angiocardiography laboratories in Iran hospitals. Mutation Research 562:1-9. (Pubitemid 38981776)
74. Zakeri F, Hirobe T. 2010. A cytogenetic approach to the effects of low levels of ionizing radiations on occupationally exposed individuals. European Journal of Radiology 73:191-195.
75. Zoetelief J, Broerse JJ. 1990. Dosimetry for radiation accidents: Present status and prospects for biological dosimeters. International Journal of Radiation Biology 57:737-750. (Pubitemid 20115271)