Construction and detection of expression vectors of microRNA-9a in BmN cells

Journal of Zhejiang University: Science B

Volumn 12, Issue 7, 2011, Pages 527-533

  Huang, Yong ^a,c,d   Zou, Quan ^b   Wang, Sheng Peng ^a,c   Tang, Shun Ming ^a,c   Zhang, Guo Zheng ^a,c   Shen, Xing Jia ^a,c  

^a JIANGSU UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b XIAMEN UNIVERSITY   (China)

^c INSTITUTE OF PLANT PROTECTION   (China)

^d HENAN UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

Bombyx mori N (BmN) Cells; EGFP gene; Expression vector; miRNA 9a (miR 9a)

Indexed keywords

ACTIN; ACTIN 3; ENHANCED GREEN FLUORESCENT PROTEIN; GENOMIC DNA; MICRORNA; MICRORNA 9A; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; EXPRESSION VECTOR; GENE EXPRESSION; GENETIC TRANSFECTION; INSECT CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SILKWORM;

ANIMALS; BLOTTING, NORTHERN; BOMBYX; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; MICRORNAS; PLASMIDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

BOMBYX MORI; EUKARYOTA;

EID: 79960455477     PISSN: 16731581     EISSN: 18621783     Source Type: Journal    
DOI: 10.1631/jzus.B1000296     Document Type: Article

References (58)

1. V. Ambros 2003 MicroRNA pathways in flies and worms: growth, death, fat, stress, and timing Cell 113 6 673 676 12809598 10.1016/S0092-8674(03)00428-8 1:CAS:528:DC%2BD3sXkvVekt7w%3D (Pubitemid 36724931)
2. D.P. Bartel 2004 MicroRNAs: genomics, biogenesis, mechanism, and function Cell 116 2 281 297 14744438 10.1016/S0092-8674(04)00045-5 1:CAS:528: DC%2BD2cXhtVals7o%3D (Pubitemid 38167318)
3. D.P. Bartel 2009 MicroRNAs: target recognition and regulatory functions Cell 136 2 215 233 19167326 10.1016/j.cell.2009.01.002 1:CAS:528: DC%2BD1MXhs1Kiuro%3D
4. F. Bejarano P. Smibert E.C. Lai 2009 miR-9a prevents apoptosis during wing development by repressing Drosophila LIM-only Dev. Biol. 338 1 63 73 19944676 10.1016/j.ydbio.2009.11.025
5. I. Biryukova J. Asmar H. Abdesselem P. Heitzler 2009 Drosophila mir-9a regulates wing development via fine-tuning expression of the LIM only factor, dLMO Dev. Biol. 327 2 487 496 19162004 10.1016/j.ydbio.2008.12.036 1:CAS:528:DC%2BD1MXis1aqt7o%3D
6. N. Bushati S.M. Cohen 2007 microRNA functions Annu. Rev. Cell Dev. Biol. 23 1 175 205 17506695 10.1146/annurev.cellbio.23.090506.123406 1:CAS:528:DC%2BD2sXhtlartr3M
7. X. Cai C.H. Hagedorn B.R. Cullen 2004 Human microRNAs are processed from capped, polyadenylated transcripts that can also function as mRNAs RNA 10 12 1957 1966 15525708 10.1261/rna.7135204 1:CAS:528:DC%2BD2cXhtVGisLjM (Pubitemid 39557833)
8. Y. Cai X. Yu Q. Zhou C. Yu H. Hu J. Liu H. Lin J. Yang B. Zhang P. Cui, et al. 2010 Novel microRNAs in silkworm (Bombyx mori) Funct. Integr. Genomics 10 3 405 415 20229201 10.1007/s10142-010-0162-7 1:CAS:528:DC%2BC3cXpt1Srsr0%3D
9. J. Cao C. Tong X. Wu J. Lv Z. Yang Y. Jin 2008 Identification of conserved microRNAs in Bombyx mori (silkworm) and regulation of fibroin L chain production by microRNAs in heterologous system Insect Biochem. Mol. Biol. 38 12 1066 1071 18977439 10.1016/j.ibmb.2008.09.008 1:CAS:528:DC%2BD1MXitVOltrY%3D
10. K. Chang S.J. Elledge G.J. Hannon 2006 Lessons from Nature: microRNA-based shRNA libraries Nat. Methods 3 9 707 714 16929316 10.1038/nmeth923 1:CAS:528:DC%2BD28XosVOnsLs%3D (Pubitemid 44275738)
11. C. Chen D.A. Ridzon A.J. Broomer Z. Zhou D.H. Lee J.T. Nguyen M. Barbisin N.L. Xu V.R. Mahuvakar M.R. Andersen, et al. 2005 Real-time quantification of microRNAs by stem-loop RT-PCR Nucleic. Acids Res. 33 20 e179 16314309 10.1093/nar/gni178 (Pubitemid 41806407)
12. C.Z. Chen L. Li H.F. Lodish D.P. Bartel 2004 MicroRNAs modulate hematopoietic lineage differentiation Science 303 5654 83 86 14657504 10.1126/science.1091903 1:CAS:528:DC%2BD3sXhtVWhs7zE (Pubitemid 38055775)
13. G. Du J. Yonekubo Y. Zeng M. Osisami M.A. Frohman 2006 Design of expression vectors for RNA interference based on miRNAs and RNA splicing FEBS J. 273 23 5421 5427 17076699 10.1111/j.1742-4658.2006.05534.x 1:CAS:528: DC%2BD2sXjvVCj (Pubitemid 44772398)
14. J. Feng K. Wang X. Liu S. Chen J. Chen 2009 The quantification of tomato microRNAs response to viral infection by stem-loop real-time RT-PCR Gene 437 1-2 14 21 19374024 10.1016/j.gene.2009.01.017 1:CAS:528:DC%2BD1MXktlGjsbc%3D
15. W. Filipowicz S.N. Bhattacharyya N. Sonenberg 2008 Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nat. Rev. Genet. 9 102 114 18197166 10.1038/nrg2290 1:CAS:528:DC%2BD1cXnt1ensA%3D%3D
16. R.C. Friedman K.K. Farh C.B. Burge D.P. Bartel 2009 Most mammalian mRNAs are conserved targets of microRNAs Genome Res. 19 1 92 105 18955434 10.1101/gr.082701.108 1:CAS:528:DC%2BD1MXptFWjuw%3D%3D
17. Y. Fukuda H. Kawasaki K. Taira 2006 Construction of microRNA-containing vectors for expression in mammalian cells Methods Mol. Biol. 338 167 173 16888358 1:CAS:528:DC%2BD28XksFOqu7w%3D
18. D. Gou H. Zhang P.S. Baviskar L. Liu 2007 Primer extension-based method for the generation of a siRNA/miRNA expression vector Physiol. Genomics 31 3 554 562 17804605 10.1152/physiolgenomics.00005.2007 1:CAS:528:DC%2BD2sXhtl2rt73M (Pubitemid 350127794)
19. J. Han Y. Lee K.H. Yeom J.W. Nam I. Heo J.K. Rhee S.Y. Sohn Y. Cho B.T. Zhang V.N. Kim 2006 Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex Cell 125 5 887 901 16751099 10.1016/j.cell.2006.03.043 1:CAS:528:DC%2BD28Xls1Shtr4%3D (Pubitemid 43810154)
20. P.A. He Z. Nie J. Chen Z. Lv Q. Sheng S. Zhou X. Gao L. Kong X. Wu Y. Jin, et al. 2008 Identification and characteristics of microRNAs from Bombyx mori BMC Genomics 9 1 248 18507836 10.1186/1471-2164-9-248 (Pubitemid 351895454)
21. X. Hong M. Hammell V. Ambros S.M. Cohen 2009 Immunopurification of Ago1 miRNPs selects for a distinct class of microRNA targets PNAS 106 35 15085 15090 19706460 10.1073/pnas.0908149106 1:CAS:528:DC%2BD1MXhtFGmsr3M
22. T. Hu Q. Fu P. Chen L. Ma O. Sin D. Guo 2009 Construction of an artificial microRNA expression vector for simultaneous inhibition of multiple genes in mammalian cells Int. J. Mol. Sci. 10 5 2158 2168 19564946 10.3390/ijms10052158 1:CAS:528:DC%2BD1MXmtlCrurk%3D
23. T. Hu P. Chen Q. Fu Y. Liu M. Ishaq J. Li L. Ma D. Guo 2010 Comparative studies of various artificial microRNA expression vectors for RNAi in mammalian cells Mol. Biotechnol. 46 1 34 40 20300885 10.1007/s12033-010-9264-7 1:CAS:528:DC%2BC3cXps1Crt74%3D
24. Y. Huang Q. Zou S.M. Tang L.G. Wang X.J. Shen 2009 Computational identification and characteristics of novel microRNAs from the silkworm (Bombyx mori L.) Mol. Biol. Rep. 37 7 3171 3176 19823945 10.1007/s11033-009-9897-4
25. G. Jagadeeswaran Y. Zheng N. Sumathipala H. Jiang E.L. Arrese J.L. Soulages W. Zhang R. Sunkar 2010 Deep sequencing of small RNA libraries reveals dynamic regulation of conserved and novel microRNAs and microRNA-stars during silkworm development BMC Genomics 11 1 52 20089182 10.1186/1471-2164-11-52
26. Y. Li F. Wang J.A. Lee F.B. Gao 2006 MicroRNA-9a ensures the precise specification of sensory organ precursors in Drosophila Genes. Dev. 20 20 2793 2805 17015424 10.1101/gad.1466306 1:CAS:528:DC%2BD28XhtFWqsbbJ (Pubitemid 44771742)
27. J. Liu 2008 Control of protein synthesis and mRNA degradation by microRNAs Curr. Opin. Cell Biol. 20 2 214 221 18329869 10.1016/j.ceb.2008.01.006 1:CAS:528:DC%2BD1cXks1Gnu7o%3D
28. S. Liu L. Zhang Q. Li P. Zhao J. Duan D. Cheng Z. Xiang Q. Xia 2009 MicroRNA expression profiling during the life cycle of the silkworm (Bombyx mori) BMC Genomics 10 1 455 19785751 10.1186/1471-2164-10-455
29. S. Liu D. Li Q. Li P. Zhao Z. Xiang Q. Xia 2010 MicroRNAs of Bombyx mori identified by Solexa sequencing BMC Genomics 11 1 148 20199675 10.1186/1471-2164-11-148
30. A. Molnar A. Bassett E. Thuenemann F. Schwach S. Karkare S. Ossowski D. Weigel D. Baulcombe 2009 Highly specific gene silencing by artificial microRNAs in the unicellular alga Chlamydomonas reinhardtii Plant J. 58 1 165 174 10.1111/j.1365-313X.2008.03767.x 1:CAS:528:DC%2BD1MXks1Cns7s%3D
31. Q.W. Niu S.S. Lin J.L. Reyes K.C. Chen H.W. Wu S.D. Yeh N.H. Chua 2006 Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance Nat. Biotechnol. 24 11 1420 1428 17057702 10.1038/nbt1255 1:CAS:528:DC%2BD28XhtFyqtLzM (Pubitemid 44722106)
32. E.A. Parizotto P. Dunoyer N. Rahm C. Himber O. Voinnet 2004 In vivo investigation of the transcription, processing, endonucleolytic activity, and functional relevance of the spatial distribution of a plant miRNA Genes Dev. 18 18 2237 2242 15371337 10.1101/gad.307804 1:CAS:528:DC%2BD2cXnslCnur8%3D (Pubitemid 39209571)
33. W. Park J. Zhai J.Y. Lee 2009 Highly efficient gene silencing using perfect complementary artificial miRNA targeting AP1 or heteromeric artificial miRNA targeting AP1 and CAL genes Plant Cell Rep. 28 3 469 480 19066901 10.1007/s00299-008-0651-5 1:CAS:528:DC%2BD1MXitlSqtLg%3D
34. R.S. Pillai S.N. Bhattacharyya W. Filipowicz 2007 Repression of protein synthesis by miRNAs: how many mechanisms? Trends Cell Biol. 17 3 118 126 17197185 10.1016/j.tcb.2006.12.007 1:CAS:528:DC%2BD2sXisleitbw%3D (Pubitemid 46357525)
35. L. Qiu H. Wang X. Xia H. Zhou Z. Xu 2008 A construct with fluorescent indicators for conditional expression of miRNA BMC Biotechnol. 8 1 77 18840295 10.1186/1472-6750-8-77
36. W. Ritchie M. Legendre D. Gautheret 2007 RNA stemloops: to be or not to be cleaved by RNAse III RNA 13 4 457 462 17299129 10.1261/rna.366507 1:CAS:528:DC%2BD2sXkt1Chtrw%3D (Pubitemid 46506928)
37. M. Rumi S. Ishihara M. Aziz H. Kazumori N. Ishimura T. Yuki C. Kadota Y. Kadowaki Y. Kinoshita 2006 RNA polymerase II mediated transcription from the polymerase III promoters in short hairpin RNA expression vector Biochem. Biophys. Res. Commun. 339 2 540 547 16300730 10.1016/j.bbrc.2005.11.037 1:CAS:528:DC%2BD2MXhtlSiur7P (Pubitemid 41739701)
38. H. Sakurai S. Izumi S. Tomino 1990 In vitro transcription of the plasma protein genes of Bombyx mori Biochim. Biophys. Acta 1087 1 18 24 2400786 1:CAS:528:DyaK3cXlsVKmurY%3D (Pubitemid 20298392)
39. M. Scherr M. Eder 2007 Gene silencing by small regulatory RNAs in mammalian cells Cell Cycle 6 4 444 449 17312397 10.4161/cc.6.4.3807 1:CAS:528:DC%2BD2sXnvFCltrk%3D (Pubitemid 46393448)
40. S. Schmollinger D. Strenkert M. Schroda 2010 An inducible artificial microRNA system for Chlamydomonas reinhardtii confirms a key role for heat shock factor 1 in regulating thermotolerance Curr. Genet. 56 4 383 389 20449593 10.1007/s00294-010-0304-4 1:CAS:528:DC%2BC3cXptVynu78%3D
41. Z. Shan Q. Lin C. Deng X. Li W. Huang H. Tan Y. Fu M. Yang X. Yu 2009 An efficient method to enhance gene silencing by using precursor microRNA designed small hairpin RNAs Mol. Biol. Rep. 36 6 1483 1489 18758992 10.1007/s11033-008- 9339-8 1:CAS:528:DC%2BD1MXmtlOgu7Y%3D
42. Z. Shan Q. Lin C. Deng Z. Zhou H. Tan Y. Fu X. Li J. Zhu L. Mai S. Kuang, et al. 2010 Comparison of approaches for efficient gene silencing induced by microRNA-based short hairpin RNA and indicator gene expression Mol. Biol. Rep. 37 4 1831 1839 19603286 10.1007/s11033-009-9618-z 1:CAS:528:DC%2BC3cXis1Wmurs%3D
43. A. Shibata A. Iwaki Y. Fukumaki 2007 A novel expression system for artificial miRNAs containing no endogenous miRNA precursor sequences J. RNAi Gene Silencing 3 1 237 247 19771221 1:CAS:528:DC%2BD2sXms1KrsLs%3D
44. J.M. Silva M.Z. Li K. Chang W. Ge M.C. Golding R.J. Rickles D. Siolas G. Hu P.J. Paddison M.R. Schlabach, et al. 2005 Second-generation shRNA libraries covering the mouse and human genomes Nat. Genet. 37 1281 1288 16200065 1:CAS:528:DC%2BD2MXhtFGhsL3N (Pubitemid 41568714)
45. G. Stefani F.J. Slack 2008 Small non-coding RNAs in animal development Nat. Rev. Mol. Cell Biol. 9 3 219 230 18270516 10.1038/nrm2347 1:CAS:528:DC%2BD1cXitlGnurY%3D (Pubitemid 351301826)
46. D. Sun M. Melegari S. Sridhar C.E. Rogler L. Zhu 2006 Multi-miRNA hairpin method that improves gene knockdown efficiency and provides linked multi-gene knockdown Biotechniques 41 1 59 63 16869514 10.2144/000112203 1:CAS:528:DC%2BD28XmvVGqurg%3D (Pubitemid 46534903)
47. H. Sun Q.W. Li X.Y. Lv J.Z. Ai Q.T. Yang J.J. Duan G.H. Bian Y. Xiao Y.D. Wang Z. Zhang, et al. 2010 MicroRNA-17 post-transcriptionally regulates polycystic kidney disease-2 gene and promotes cell proliferation Mol. Biol. Rep. 37 6 2951 2958 19821056 10.1007/s11033-009-9861-3 1:CAS:528: DC%2BC3cXosFCks7g%3D
48. G. Tang X. Tang V. Mendu X. Jia Q.J. Chen L. He 2008 The art of microRNA: various strategies leading to gene silencing via an ancient pathway Biochim. Biophys. Acta 1779 11 655 662 18620087 1:CAS:528:DC%2BD1cXhtlGgsrrM
49. C.Z. Tong Y.F. Jin Y.Z. Zhang 2006 Computational prediction of microRNA genes in silkworm genome J. Zhejiang Univ.-Sci. B 7 10 806 816 16972323 10.1631/jzus.2006.B0806 1:CAS:528:DC%2BD28Xht1WmsrzM
50. R.D. Trujillo S.B. Yue Y. Tang W.E. O'Gorman C.Z. Chen 2010 The potential functions of primary microRNAs in target recognition and repression EMBO J. 29 19 3272 3285 20808284 10.1038/emboj.2010.208 1:CAS:528:DC%2BC3cXhtV2hsbjI
51. H. Vaucheret F. Vazquez P. Crete D.P. Bartel 2004 The action of ARGONAUTE1 in the miRNA pathway and its regulation by the miRNA pathway are crucial for plant development Genes Dev. 18 10 1187 1197 15131082 10.1101/gad.1201404 1:CAS:528:DC%2BD2cXktlCqs7s%3D (Pubitemid 38669039)
52. J. Xu J.Q. Zeng G. Wan G.B. Hu H. Yan L.X. Ma 2009 Construction of siRNA/miRNA expression vectors based on a one-step PCR process BMC Biotechnol. 9 1 53 19490634 10.1186/1472-6750-9-53
53. X. Yu Q. Zhou S.C. Li Q. Luo Y. Cai W.C. Lin H. Chen Y. Yang S. Hu J. Yu 2008 The silkworm (Bombyx mori) microRNAs and their expressions in multiple developmental stages PLoS One 3 8 e2997 18714353 10.1371/journal.pone.0002997
54. X. Yu Q. Zhou Y. Cai Q. Luo H. Lin S. Hu J. Yu 2009 A discovery of novel microRNAs in the silkworm (Bombyx mori) genome Genomics 94 6 438 444 19699294 10.1016/j.ygeno.2009.08.007 1:CAS:528:DC%2BD1MXhsVWnsbzK
55. Y. Zeng B.R. Cullen 2005 Efficient processing of primary microRNA hairpins by Drosha requires flanking nonstructured RNA sequences J. Biol. Chem. 280 30 27595 27603 15932881 10.1074/jbc.M504714200 1:CAS:528: DC%2BD2MXmsVyqt74%3D (Pubitemid 41076871)
56. Y. Zeng E.J. Wagner B.R. Cullen 2002 Both natural and designed microRNAs can inhibit the expression of cognate mRNAs when expressed in human cells Mol. Cell 9 6 1327 1333 12086629 10.1016/S1097-2765(02)00541-5 1:CAS:528: DC%2BD38Xlt1Sktrk%3D (Pubitemid 34722311)
57. Y. Zhang X. Zhou X. Ge J. Jiang M. Li S. Jia X. Yang Y. Kan X. Miao G. Zhao, et al. 2009 Insect-specific microRNA involved in the development of the silkworm Bombyx mori PLoS One 4 3 e4677 19262741 10.1371/journal.pone.0004677
58. H. Zhou X.G. Xia Z. Xu 2005 An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi Nucleic Acids Res. 33 6 e62 15805121 10.1093/nar/gni061 (Pubitemid 41748359)