An efficient method to enhance gene silencing by using precursor microRNA designed small hairpin RNAs

Molecular Biology Reports

Volumn 36, Issue 6, 2009, Pages 1483-1489

  Shan, Zhixin ^a,b   Lin, Qiuxiong ^a,b   Deng, Chunyu ^a,b   Li, Xiaohong ^a,b   Huang, Wei ^a,b   Tan, Honghong ^a,b   Fu, Yongheng ^a,b   Yang, Min ^a,b   Yu, Xi Yong ^a  

^a GUANGDONG PROVINCIAL PEOPLE'S HOSPITAL   (China)

^b Guangdong Provincial Cardiovascular Institute   (China)

Author keywords

MicroRNAs; RNA interference; RNA Pol II promoter; Small hairpin RNA

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GREEN FLUORESCENT PROTEIN; MESSENGER RNA PRECURSOR; MICRORNA; RNA POLYMERASE II; SHORT HAIRPIN RNA;

ARTICLE; CELL STRAIN HEK293; COMPARATIVE STUDY; CONTROLLED STUDY; EMBRYO; FLUORESCENCE MICROSCOPY; GENE SILENCING; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PROMOTER REGION; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA VECTOR; WESTERN BLOTTING;

CELL LINE; GENE SILENCING; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; GREEN FLUORESCENT PROTEINS; HUMANS; METHODS; MICRORNAS; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 67651102784     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-008-9339-8     Document Type: Article

References (15)

1. A Fire S Xu MK Montgomery SA Kostas SE Driver CC Mello 1998 Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans Nature 391 806 811 10.1038/35888 (Pubitemid 28099681)
2. G Meister T Tuschl 2004 Mechanisms of gene silencing by double-stranded RNA Nature 431 343 349 10.1038/nature02873 (Pubitemid 39265674)
3. PD Zamore T Tuschl PA Sharp DP Bartel 2000 RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21 to 23 nucleotide intervals Cell 101 25 33 10.1016/S0092-8674(00)80620-0
4. SM Hammond AA Caudy GJ Hannon 2001 Posttranscriptional gene silencing by double-stranded RNA Nat Rev Genet 2 110 119 10.1038/35052556 (Pubitemid 33674002)
5. SM Hammond E Bernstein D Beach GJ Hannon 2000 An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells Nature 404 293 296 10.1038/35005107 (Pubitemid 30163547)
6. G Hutvagner PD Zamore 2002 A microRNA in a multiple-turnover RNAi enzyme complex Science 297 2056 2060 10.1126/science.1073827 (Pubitemid 35052535)
7. BR Cullen 2004 Transcription and processing of human microRNA precursors Mol Cell 16 861 865 10.1016/j.molcel.2004.12.002 (Pubitemid 40018397)
8. VN Kim 2005 MicroRNA biogenesis: coordinated cropping and dicing Nat Rev Mol Cell Biol 6 376 385 10.1038/nrm1644 (Pubitemid 40637855)
9. S Tsuchiya Y Okuno G Tsujimoto 2006 MicroRNA: biogenetic and functional mechanisms and involvements in cell differentiation and cancer J Pharmacol Sci 101 267 270 10.1254/jphs.CPJ06013X (Pubitemid 44285172)
10. D Boden O Pusch R Silbermann F Lee L Tucker B Ramratnam 2004 Enhanced gene silencing of HIV-1 specific siRNA using microRNA designed hairpins Nucleic Acids Res 32 1154 1158 10.1093/nar/gkh278 (Pubitemid 38854717)
11. F Stegmeier G Hu RJ Rickles GJ Hannon SJ Elledge 2005 A lentiviral microRNA-based system for single-copy polymerase II-regulated RNA interference in mammalian cells Proc Natl Acad Sci USA 102 13212 13217 10.1073/pnas. 0506306102 (Pubitemid 41325003)
12. Y Zeng EJ Wagner BR Cullen 2002 Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells Mol Cell 9 1327 1333 10.1016/S1097-2765(02)00541-5 (Pubitemid 34722311)
13. Y Zeng BR Cullen 2004 Structural requirements for pre-microRNA binding and nuclear export by Exportin 5 Nucleic Acids Res 32 4776 4785 10.1093/nar/gkh824 (Pubitemid 39429682)
14. JM Silva MZ Li K Chang W Ge MC Golding RJ Rickles 2005 Second-generation shRNA libraries covering the mouse and human genomes Nat Genet 37 1281 1288
15. J Yuan X Wang Y Zhang X Hu X Deng J Fei 2006 shRNA transcribed by RNA Pol II promoter induce RNA interference in mammalian cell Mol Biol Rep 33 43 49 10.1007/s11033-005-3965-1