Comparison of approaches for efficient gene silencing induced by microRNA-based short hairpin RNA and indicator gene expression

Molecular Biology Reports

Volumn 37, Issue 4, 2010, Pages 1831-1839

  Shan, Z X ^a,b,c   Lin, Q X ^a,b   Deng, C Y ^a,b   Zhou, Z L ^a,b   Tan, H H ^a,b   Fu, Y H ^a,b   Li, X H ^a,b   Zhu, J N ^a,b   Mai, L P ^a,b   Kuang, S J ^a,b   Lin, S G ^a,b   Yu, X Y ^a,b  

^a GUANGDONG PROVINCIAL PEOPLE'S HOSPITAL   (China)

^b Guangdong Provincial Cardiovascular Institute   (China)

^c SHANTOU UNIVERSITY MEDICAL COLLEGE   (China)

Author keywords

Dual luciferase reporter assay system; Green fluorescence protein; MicroRNAs; Real time quantitative PCR; Red fluorescence protein; RNA interference

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GREEN FLUORESCENT PROTEIN; MICRORNA; RED FLUORESCENT PROTEIN; SHORT HAIRPIN RNA; LUCIFERASE; MESSENGER RNA; MIRN155 MICRORNA, HUMAN; PHOTOPROTEIN; SMALL INTERFERING RNA;

ARTICLE; CONTROLLED STUDY; EXPRESSION VECTOR; GENE CASSETTE; GENE EXPRESSION; GENE SILENCING; HUMAN; HUMAN CELL; IN VITRO STUDY; INTRON; NUCLEOTIDE SEQUENCE; PLASMID; RNA INTERFERENCE; CELL LINE; CHEMISTRY; COMPARATIVE STUDY; CONFORMATION; GENE EXPRESSION REGULATION; GENETIC PROCEDURES; GENETIC TRANSFECTION; GENETICS; METABOLISM; MOLECULAR GENETICS;

BASE SEQUENCE; CELL LINE; GENE EXPRESSION REGULATION; GENE SILENCING; GENETIC TECHNIQUES; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; GREEN FLUORESCENT PROTEINS; HUMANS; LUCIFERASES; LUMINESCENT PROTEINS; MICRORNAS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; PLASMIDS; RNA, MESSENGER; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 77953173672     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-009-9618-z     Document Type: Article

References (21)

1. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391:806-811
2. Meister G, Tuschl T (2004) Mechanisms of gene silencing by double-stranded RNA. Nature 431:343-349
3. Hutvagner G, Zamore PD (2002) A microRNA in a multipleturnover RNAi enzyme complex. Science 297:2056-2060
4. Cullen BR (2004) Transcription and processing of human microRNA precursors. Mol Cell 16:861-865
5. Kim VN (2005) MicroRNA biogenesis: Coordinated cropping and dicing. Nat Rev Mol Cell Biol 6:376-385
6. Cullen BR (2005) RNAi the natural way. Nat Genet 37: 1163-1165
7. Silva JM, Li MZ, Chang K, Ge W, Golding MC, Rickles RJ, Siolas D, Hu G, Paddison PJ, Schlabach MR, Sheth N, Bradshaw J, Burchard J, Kulkarni A, Cavet G, Sachidanandam R, McCombie WR, Cleary MA, Elledge SJ, Hannon GJ (2005) Second-generation shRNA libraries covering the mouse and human genomes. Nat Genet 37:1281-1288
8. Stegmeier F, Hu G, Rickles RJ, Hannon GJ, Elledge SJ (2005) A lentiviral microRNA-based system for single-copy polymerase IIregulated RNA interference in mammalian cells. Proc Natl Acad Sci USA 102:13212-13217
9. Dickins RA, Hemann MT, Zilfou JT, Simpson DR, Ibarra I, Hannon GJ, Lowe SW (2005) Probing tumor phenotypes using stable and regulated synthetic microRNA precursors. Nat Genet 37:1289-1295
10. Chung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. Nucleic Acids Res 34:e53
11. Shan Z, Lin Q, Deng C, Li X, Huang W, Tan H, Fu Y, Yang M, Yu XY (2008) An efficient method to enhance gene silencing by using precursor microRNA designed small hairpin RNAs. Mol Biol Rep. doi: 10.1007/s11033-008-9339-8
12. Shan ZX, Lin QX, Yang M, Deng CY, Kuang SJ, Zhou ZL, Xiao DZ, Liu XY, Lin SG, Yu XY (2009) A quick and efficient approach for gene silencing by using triple putative microRNA-based short hairpin RNAs. Mol Cell Biochem 323: 81-89
13. Du G, Yonekubo J, Zeng Y, Osisami M, Frohman MA (2006) Design of expression vectors for RNA interference based on miRNAs and RNA splicing. FEBS J 273:5421-5427
14. Qiu L, Wang H, Xia X, Zhou H, Xu Z (2008) A construct with fluorescent indicators for conditional expression of miRNA. BMC Biotechnol 8:77
15. Hammond SM, Caudy AA, Hannon GJ (2001) Posttranscriptional gene silencing by double-stranded RNA. Nat Rev Genet 2: 110-119
16. Pfaffl MW (2001) A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: e45
17. Tsuchiya S, Okuno Y, Tsujimoto G (2006) MicroRNA: biogenetic and functional mechanisms and involvements in cell differentiation and cancer. J Pharmacol Sci 101:267-270
18. Buchman AR, Berg P (1988) Comparison of intron-dependent and intron independent gene expression. Mol Cell Biol 8:4395-4405
19. Huang MTF, Gorman CM (1990) Intervening sequences increase efficiency of RNA 30 processing and accumulation of cytoplasmic RNA. Nucl Acids Res 18:937-947
20. Liu YP, Haasnoot J, ter Brake O, Berkhout B, Konstantinova P (2008) Inhibition of HIV-1 by multiple siRNAs expressed from a single microRNA polycistron. Nucleic Acids Res 36:2811-2824
21. Grimm D, Streetz KL, Jopling CL, Storm TA, Pandey K, Davis CR, Marion P, Salazar F, Kay MA (2006) Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Nature 441:537-541