Construction of siRNA/miRNA expression vectors based on a one-step PCR process

BMC Biotechnology

Volumn 9, Issue , 2009, Pages

  Xu, Jun ^a   Zeng, Jie Qiong ^a   Wan, Gang ^a   Hu, Gui Bin ^a   Yan, Hong ^a   Ma, Li Xin ^a  

^a HUBEI UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURACY RATE; E. COLI; EXPRESSION VECTORS; HIGH-THROUGHPUT; HIGH-THROUGHPUT ASSAYS; HOMOLOGOUS RECOMBINATION; HUMAN DISEASE; IN-VIVO; LINEAR EXPRESSION; LONG OLIGONUCLEOTIDE; MAMMALIAN CELLS; PCR PRODUCTS; RNA INTERFERENCE; TARGET GENE EXPRESSION; UV LIGHT;

ESCHERICHIA COLI; GENE EXPRESSION; LIBRARIES; MAMMALS; NUCLEIC ACIDS; OLIGONUCLEOTIDES; RNA; SYNTHESIS (CHEMICAL);

VECTORS;

MICRORNA; OLIGONUCLEOTIDE; SMALL INTERFERING RNA; PRIMER DNA;

ACCURACY; ARTICLE; BACTERIAL METABOLISM; CELL STRAIN HEK293; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE LIBRARY; GENE MUTATION; GENE SILENCING; GENETIC TRANSFORMATION; GENOME SIZE; HELA CELL; HIGH THROUGHPUT SCREENING; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; IN VIVO STUDY; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE METABOLISM; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; ULTRAVIOLET RADIATION; WESTERN BLOTTING; GENE EXPRESSION; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; METHODOLOGY;

MAMMALIA;

DNA PRIMERS; GENE EXPRESSION; GENE LIBRARY; GENETIC VECTORS; HELA CELLS; HUMANS; MICRORNAS; POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 67649506336     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-53     Document Type: Article

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