Affinity purification of a chimeric nicotinic acetylcholine receptor in the agonist and antagonist bound states

Protein Expression and Purification

Volumn 79, Issue 1, 2011, Pages 102-110

  Liu, Shenping ^a   Babcock, Merrill S ^a   Bode, Jacob ^a   Chang, Jeanne S ^a   Fischer, H David ^a   Garlick, Robert L ^a   Gill, Gurnam S ^a   Lund, Eric T ^a   Margolis, Brandon J ^a   Mathews, W Rodney ^a   Rogers, Bruce N ^a   Wolfe, Mark ^a   Groppi, Vince ^a   Baldwin, Eric T ^a,b  

^a PFIZER GLOBAL RESEARCH AND DEVELOPMENT   (United States)

^b BRISTOL MYERS SQUIBB   (United States)

Author keywords

Affinity purification; Nicotinic acetylcholine receptor; Protein expression

Indexed keywords

BUNGAROTOXIN RECEPTOR; HYBRID PROTEIN; NICOTINIC AGENT; NICOTINIC RECEPTOR; NICOTINIC RECEPTOR BLOCKING AGENT;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CELL STRAIN HEK293; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; MOUSE; PROTEIN BINDING; ULTRACENTRIFUGATION;

AMINO ACID SEQUENCE; ANIMALS; CHROMATOGRAPHY, AFFINITY; HEK293 CELLS; HUMANS; MICE; MOLECULAR SEQUENCE DATA; NICOTINIC AGONISTS; NICOTINIC ANTAGONISTS; PROTEIN BINDING; RECEPTORS, NICOTINIC; RECOMBINANT FUSION PROTEINS; ULTRACENTRIFUGATION;

EID: 79960127642     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2011.05.019     Document Type: Article

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