Design and expression of human α7 nicotinic acetylcholine receptor extracellular domain mutants with enhanced solubility and ligand-binding properties

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1794, Issue 2, 2009, Pages 355-366

  Zouridakis, Marios ^a,b   Zisimopoulou, Paraskevi ^a   Eliopoulos, Elias ^c   Poulas, Konstantinos ^b   Tzartos, Socrates J ^a,b  

^a HELLENIC PASTEUR INSTITUTE   (Greece)

^b UNIVERSITY OF PATRAS   (Greece)

^c AGRICULTURAL UNIVERSITY OF ATHENS   (Greece)

Author keywords

3D model; Circular dichroism spectroscopy; Dynamic light scattering; Electron microscopy; Human 7 nicotinic acetylcholine receptor extracellular domain; Ligand binding

Indexed keywords

ACETYLCHOLINE BINDING PROTEIN; ALPHA BUNGAROTOXIN; BINDING PROTEIN; NICOTINE; NICOTINIC ACETYLCHOLINE RECEPTOR ALPHA 7; NICOTINIC RECEPTOR; TUBOCURARINE CHLORIDE; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CIRCULAR DICHROISM; CONTROLLED STUDY; CRYSTAL STRUCTURE; DEGLYCOSYLATION; ELECTRON MICROSCOPY; GEL FILTRATION; GLYCOSYLATION; HUMAN; HYDROPHILICITY; LIGAND BINDING; LIGHT SCATTERING; PICHIA PASTORIS; POINT MUTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN STRUCTURE; SEQUENCE ALIGNMENT;

AMINO ACID SEQUENCE; ANIMALS; BUNGAROTOXINS; GLYCOSYLATION; HUMANS; LIGANDS; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTATION; NICOTINE; NICOTINIC AGONISTS; NICOTINIC ANTAGONISTS; PICHIA; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RADIOLIGAND ASSAY; RECEPTORS, NICOTINIC; SOLUBILITY; TORPEDO; TUBOCURARINE;

PICHIA PASTORIS;

EID: 58149193160     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2008.11.002     Document Type: Article

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